In this demonstration cuvette is filled with chemical (for measurements 3/4 cuvette is enough). Some times when using with full cuvette, the chemical gets spilled into the cuvette holder. If the chemical is highly corrosive, it attacks the inside electronics and optics of the spectrophotometer. As a precaution, it is better not to use full cuvette chemicals and also better to use a cap for the cuvette (it is available). I have seen this problem before and sharing this with the students and the instructors.
impressive presentation ... for one & all new to spec's . now i want to see more vid's you've posted. by showing the lo - A end of the spec's ability to measure dilute solutions an answer to my question is had. i wanted to know this in order to test a tlc strip's ability to detect ug amounts of a cd. also found it interesting your method has you re-use the same cuvette. thanks too for the rundown of the prep'n of each flask with those iron reagents. this will call for some homework study to understand why this is done. and to learn what a test of the un-altered iron would have told you.
That Spectrovis thing is cool. Its alot better than the spectrophoto meter machine its biger and havier this one is simple and easy to use. It must be expansive. Thank you for the video:)
good afternoon, good video. I can determine any colored salt with this technique? I want to analyze Au, Cu, among others. It is possible with this technique thank you
When it was said "Wipe the Glassware container down if you touch it with your hand," may I assume the comment was made to refer to an "ungloved' hand but touching with a "Clean Gloved Hand" is OK? I am new to this.
hi there, i want to ask you is it possible to measure the number of ferrous iron? I work with the bacteria which able to reduce ferric iron into ferrous iron in the anaerobic condition. And i have a difficulties to estimate the number of ferrous that produced. Because i thought that ferrous iron inside the medium which put in the anaerobic condition will oxidized directly when it's contact with oxygen in the aerobic condition. Is it wrong? thank you
+Kristin Widyasari Hi Kristin. You can measure ferrous ions by difference. You take an amount of your solution, you oxidize the iron ions you have in solution to Fe3+ and measure how total iron ions. After that you realize the purge procedure in another amount of your solution (the same, but another aliquot) and measure the total ferric ions without preoxidation of the iron ions (u can use a N2 or argon atmosphere). The difference is the amount of ferrous ions. Bye (PS.: Sorry my english. This language is not my native one)
Kristin Widyasari You're welcome, Kristin. You can use some electroanalytical method, too. Amperometry is a good choice, so you can measure the concentration of Fe2+ and Fe3+ ions in real time while your biological experiment is proceeding. Wish you to have a great day, too.
@@syedmbilal7741 There are several reasons your spectrophotometer could be larger. It likely depends on the type of source and the type of detector your particular instrument has. Diode array instruments are typically preferred for teaching instruments, as they have few moving parts and are quick and relatively cheap. Instruments containing monochromators are preferred for higher quality measurements (like those in a research lab).
Yes, but you'll need to extract the iron from the food. Typically high iron foods like spinach work fairly well. The Harris Quantitative Analysis book has a procedure for such an analysis.
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When it is covid then u have to do the virtual lab...
Thank you. Clear and nice video. It was very useful in my learning. Really cool!
In this demonstration cuvette is filled with chemical (for measurements 3/4 cuvette is enough). Some times when using with full cuvette, the chemical gets spilled into the cuvette holder. If the chemical is highly corrosive, it attacks the inside electronics and optics of the spectrophotometer. As a precaution, it is better not to use full cuvette chemicals and also better to use a cap for the cuvette (it is available). I have seen this problem before and sharing this with the students and the instructors.
thanks! was watching this and wondered about that
Why buffer is not added?
Is orthophenanthroline a chromophore in the detection of iron in the sample
Hi, I want to ask you about Why do you don't use hidroksilamin, fenantrolin and buffer in determination of iron ?
Cool video! How is the accuracy of the usb spectrometer?
Hi, how much the reagents needed, what are the ratios?
Nice vid anw
Can u show how u made those orange solutions ?😢
What is TPTZ mathod for iron testing
Thank you for this presentation
upto 3/4 right.
is this so you don’t drink unfiltered water? water with iron?
impressive presentation ... for one & all new to spec's . now i want to see more vid's you've posted.
by showing the lo - A end of the spec's ability to measure dilute solutions an answer to my question is had.
i wanted to know this in order to test a tlc strip's ability to detect ug amounts of a cd.
also found it interesting your method has you re-use the same cuvette.
thanks too for the rundown of the prep'n of each flask with those iron reagents.
this will call for some homework study to understand why this is done. and to learn
what a test of the un-altered iron would have told you.
That Spectrovis thing is cool. Its alot better than the spectrophoto meter machine its biger and havier this one is simple and easy to use. It must be expansive. Thank you for the video:)
I would say its way cheaper
can u pls upload a video for the estimation of phosphate
Can u make a PDF of the Procedure please.
wonderful 💗
They actually do. I always forget to watch the videos, and I'm constantly reminded by Mastaneh or one of the TAs to view them.
Can i know where to download the logger pro software for free?
thepiratebay.org
how make seriers of standard
how to determine zinc on spectrophotometer
it is possible by titration
good ..
good afternoon, good video.
I can determine any colored salt with this technique?
I want to analyze Au, Cu, among others. It is possible with this technique
thank you
+Alex Pires o-Phenatroline forms a complex specifically with Fe2+ only
i want to know standard operating procedure to test iron for raw water as per pharmaguideline
@@MITHILESHKUMAR-zf9mhsame here
When it was said "Wipe the Glassware container down if you touch it with your hand," may I assume the comment was made to refer to an "ungloved' hand but touching with a "Clean Gloved Hand" is OK? I am new to this.
hi there, i want to ask you is it possible to measure the number of ferrous iron? I work with the bacteria which able to reduce ferric iron into ferrous iron in the anaerobic condition. And i have a difficulties to estimate the number of ferrous that produced. Because i thought that ferrous iron inside the medium which put in the anaerobic condition will oxidized directly when it's contact with oxygen in the aerobic condition. Is it wrong? thank you
+Kristin Widyasari Hi Kristin. You can measure ferrous ions by difference. You take an amount of your solution, you oxidize the iron ions you have in solution to Fe3+ and measure how total iron ions. After that you realize the purge procedure in another amount of your solution (the same, but another aliquot) and measure the total ferric ions without preoxidation of the iron ions (u can use a N2 or argon atmosphere). The difference is the amount of ferrous ions.
Bye
(PS.: Sorry my english. This language is not my native one)
thank you nailson..:D, wish you to have a great day.
Kristin Widyasari You're welcome, Kristin. You can use some electroanalytical method, too. Amperometry is a good choice, so you can measure the concentration of Fe2+ and Fe3+ ions in real time while your biological experiment is proceeding.
Wish you to have a great day, too.
Hello can I ask the materials and procedure used in your experiment. Thank you.
Do you have any reference papers for this iron determination analysis?
did you find any?
Why is our spectrometer so big ?
I wonder too
@@syedmbilal7741 There are several reasons your spectrophotometer could be larger. It likely depends on the type of source and the type of detector your particular instrument has. Diode array instruments are typically preferred for teaching instruments, as they have few moving parts and are quick and relatively cheap. Instruments containing monochromators are preferred for higher quality measurements (like those in a research lab).
Tq
Very helpful thank you so much 😊
Someone in my lab mixed ammonium thiocyante solution with iron solution and we had to repeat for three times.
how unknown solution is prepared
Information ❤
when u school cant afford the equipment smh
Equipment are very expensive specially the vatimin analysis
We got a huge spectrophometre
hi guys. any one has procedure of Iron analysis in serum send it to me.
Thank you.
Is it possible that we use this method for solid food products to measure the amount of Fe?
no.
Yes. You would need to dissolve the solid food in HCL and add complexing agents
Yes, but you'll need to extract the iron from the food. Typically high iron foods like spinach work fairly well. The Harris Quantitative Analysis book has a procedure for such an analysis.
Hit students hit like
Hi madam, am so interesting. how can i get the instrument
Hayelom Kassa shut up
@@dwtrowe how dare you, its cause of people like you the world is a horrible place
Red lamp in the bedroom... OVAL LIGHT IN THE OFFICE.... AND SEEING WHICH BURNS OUT AFTER A LONG TIME.
"abuwt"
She's canadian isn;t she
I immediately googled the university name as soon as I heard the abuwt, and yes, Canadian indeed
Carleton University lol
Ahhhh never handle cuvettes with your bare hands. The video is great otherwise.
There are sides of a cuvette for bare fingers.
I don't know; why don't you ask it? AHAHAHAHAHAHAHA!!!! :)
who here in 2017 LOL
who here in 2017 6AM LOL
im in 2019 lmao
Gloves
Even though it may seem insignificant. Your hands are adding some heat to the flask. Maybe they don't teach tat in University anymore.
Hi, For your next video, please use gloves. thanks.
she is one hot scientist.
not as hot as u
oh thank you ! im blushing under my face mask.
That's one sexy face mask
thanks, you must be very kinky
stupid
1,10 phenonthroline mathod is not accurate
she so bad