Sound cuts out at 420 exactly for about a minute, which pertains to my question of do you have any advice you could give for cannabis or hemp tissue culture
You mentioned running the pumps for 5 minutes every 6 hours…. Do you synchronize the start of the light cycle with a nutrient feed? Or is it not a critical aspect of these systems?
No, plants will be submerged in liquid media for some time and then media will again be transferred to another container. This is the principle of temporary immersion bioreactor.
Sound cuts out at 420 exactly for about a minute, which pertains to my question of do you have any advice you could give for cannabis or hemp tissue culture
How’d the ping do? That’s what I was to try. Are you filtering the air coming in from the diaphragm pump?
You mentioned running the pumps for 5 minutes every 6 hours…. Do you synchronize the start of the light cycle with a nutrient feed? Or is it not a critical aspect of these systems?
I like it. Please explain about maintaining constant humidity in the bioreactors. Some ideas.Thanks
The bioreactors are sealed, close to 100% humidity
What îs the size of filters ? 1 micron ? 0,22 microns ?
0.22 micros
@@PlantCellTechnology thank you.
Couldn't you use a check valve for the green hose?
Is you use humidifier in your growing room
Do you need anything to anchor the plant? Or do you just let them float ?
No, plants will be submerged in liquid media for some time and then media will again be transferred to another container. This is the principle of temporary immersion bioreactor.
@@PlantCellTechnology is there any reason you couldn’t initiate a tib system with a node as an explant?
@@jasonwalters7896 we need to confirm this Francisco as he was the one working on the system.
Also, sorry to be a bother, but does the liquid media in a TIB system contain the same quantity / ratio of PGRs that would be in a gelled media?
@@jasonwalters7896 Yes, same ratio and quantity of PGRs.
Just For 2 bioreactors : what electric Power the pomps must have ? And the other components ?
In general, you need 0.5-1.0 watts per bioreactor of this size. I use two - 4 watts aquarium pumps.
@@PlantCellTechnology thank you
good that we heard the explanation.... xD
Very interesting. I would be very interested in getting one. Could you tell me if you sell one of these TIBs?
@@PlantCellTechnology Thanks you! We will keep it in mind
These bioreactors are from a French company. www.plantform.se/pub/