how to transform E coli by heat shock

Поделиться
HTML-код
  • Опубликовано: 7 сен 2024
  • A synthetic biology mooc sponsored by Mairie de Paris, Fondation Liliane Bettencourt Schueller, Citizen Cyberlab FP7 produced by mooc factory CRI Paris.

Комментарии • 19

  • @anglman3234
    @anglman3234 5 лет назад +5

    Very well explained. I like your presentation, very nice and simple without the burden of heavy jargons. You deserve an award for the best teacher

    • @raslalique
      @raslalique 3 года назад

      However, if you keep skipping the jargon you'll learn a lot slower in the long run.

  • @KJKP
    @KJKP 3 года назад +1

    I really enjoyed the instructional style of the presenter.

  • @santelmo5222
    @santelmo5222 7 лет назад +4

    Lights, cells, action!

  • @spectator59
    @spectator59 2 года назад +1

    Good content and style. However, is the sterile technique used here really good enough?

  • @duyduong8519
    @duyduong8519 4 года назад +1

    Thank you! I learned interesting knowledge from you.

    • @daxtonjabari3088
      @daxtonjabari3088 3 года назад

      You probably dont give a damn but does any of you know of a method to get back into an instagram account..?
      I was stupid forgot the account password. I would love any help you can give me.

    • @camiloramon3477
      @camiloramon3477 3 года назад

      @Daxton Jabari instablaster :)

    • @daxtonjabari3088
      @daxtonjabari3088 3 года назад

      @Camilo Ramon thanks so much for your reply. I found the site thru google and I'm trying it out now.
      I see it takes a while so I will reply here later with my results.

    • @daxtonjabari3088
      @daxtonjabari3088 3 года назад

      @Camilo Ramon it worked and I actually got access to my account again. I'm so happy!
      Thanks so much, you really help me out!

    • @camiloramon3477
      @camiloramon3477 3 года назад

      @Daxton Jabari you are welcome :D

  • @kazmkucuk1663
    @kazmkucuk1663 2 года назад

    Cold environment during transformation maintain cell membrane movement stability. Thus increases chance of plasmid to migrate into cell.

  • @acrobacia
    @acrobacia 3 года назад

    MADRE MIA!!

  • @LeonardoGomez-wb6jr
    @LeonardoGomez-wb6jr Год назад

    Is the LB media added with the antibiotic?

  • @rajghosh4485
    @rajghosh4485 2 года назад

    How do we calculate the transformation efficiency by following this protocol?

  • @PRITAM0000
    @PRITAM0000 4 года назад

    খুব ভালো

  • @anuradhapandit9824
    @anuradhapandit9824 7 лет назад

    After aliquoting from the 200 ul competent cells, can you refreeze it? Does it effect the cells in anyway? Thanks....

  • @alukooluseun8661
    @alukooluseun8661 4 года назад

    please what are the beads for?

    • @KJKP
      @KJKP 3 года назад +2

      The beads behave as vectors to distribute the bacteria.