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Cuvette need not be full. It should be at that level where light will be able to pass completely. So even if the cuvette is only filled half or more than half and still it will give results.
NAD+ doesn't have any epsilon value so overall 0x 0.2x 0.5 cm would have been zero. But let us say NAD+ have some amount of epsilon value and also concentration is different that is 0.2 mM. Then first take absorbance of NADH separately and then absorbance of NAD+ and simply add them as absorbance is additive in nature.
Enroll to attend CSIR NET Life Science Classroom Coaching: ifasonline.com/life-science-classroom-coaching.jsp
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To get CSIR NET Life Science Online Test Series: ifasonline.com/life-science-online-test-series-plan.jsp
Free Practice Questions: ifasonline.com/free-online-test-series-for-life-science.jsp
☎️ For more details call us on: 8600734656 / 8956123798
/ 91722-66888
Thank you sir very helpful video
Other types pe bhi video banaiye spectroscopy ke
Thank you sir... Thank you.. Very nice informative lecture sir 🙂🙂👍
Thanks & Welcome
Good morning sir
So cuvette need to be fill full or any amount will give the desired results?
𝐓𝐨 𝐠𝐞𝐭 𝐝𝐞𝐭𝐚𝐢𝐥𝐞𝐝 𝐢𝐧𝐟𝐨 𝐚𝐛𝐨𝐮𝐭 𝐂𝐨𝐮𝐫𝐬𝐞𝐬 𝐟𝐫𝐨𝐦 𝐨𝐮𝐫 𝐀𝐜𝐚𝐝𝐞𝐦𝐢𝐜 𝐄𝐱𝐩𝐞𝐫𝐭𝐬, 𝐩𝐥𝐞𝐚𝐬𝐞 𝐟𝐢𝐥𝐥 𝐨𝐮𝐭 𝐭𝐡𝐞 𝐟𝐨𝐫𝐦: bit.ly/CSIRNETLS
Cuvette need not be full. It should be at that level where light will be able to pass completely. So even if the cuvette is only filled half or more than half and still it will give results.
Sir suppose NADH was at 0.1 but NAD+ was at 0.2 then what should we do ?
NAD+ doesn't have any epsilon value so overall 0x 0.2x 0.5 cm would have been zero. But let us say NAD+ have some amount of epsilon value and also concentration is different that is 0.2 mM. Then first take absorbance of NADH separately and then absorbance of NAD+ and simply add them as absorbance is additive in nature.