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I like this tutorial, just wish an alternate angle was filmed for gently rinsing the dye off the slide.

Great resource. Clear and simple demonstration! Good job on the videography and lighting; made everything easy to see. Thanks!

Can we take a colonie of bacteria from a gel plate and mix it with a few drops of methyl red in a well plate? Or does it have to be broth?

Steps: 1) Label glass smear in corner with culture name 2) Transfer small drop of water aseptically on center of slip using wire loop 3) Aseptically take a small quantity of a bacteria colony from agar plate 4) Transfer bacteria culture to water drop on slide, and spread around till the water doesn't look clear 5) Allow smear to air dry 6) Once completely dry, heat fix smear by passing slide (facing upward) over the Bunsen flame 3 times.

should be done in a ISO class 5 HOOD?

Good 😃😃😃😃😊😊😊😊

Why are the agar plates labelled?

Marvelous

Can we use tubes instead of microtitre plate fr performing MICtesting

Thank you! Im doing a senior project and this video is very helpful.

Thanks a Lot <3

why is it called japanese gram test?

Wow you can literally see the DNA

can u plz tell me why have you taken 2ml of TSB and 2 ml of nalidixic acid solution. if the stock solution of nalidixic acid is 2048ug/ml. then how can we calculate how much ul of the antibiotic needs to be taken so that the final conc of nalidixic acid becomes 1ug/ml??

very informative, but I guess u can make the handling easier and less complicated by using serial dilution tubes

Amazing explanation

Very nice and straightforward. Do you have a written protocol of this procedure, I would like to try and replicate it in my lab.

Why are the plates stacked?

Thankk you very much...very helpful

on 5:16 what test is it? I couldn't get the words

Very understandable, thank you sir

great

Are these traditional methods for bacterial identification capable of identifying the exact species?

Amazing

I wished to see the result

these are good but the music in the background is distracting and unnecessary.

Informative video

Informative video

Media or which nurite agar in plates..??

Very informative and to the point.

Which glass marker you used

But why do we label around the rim?

is there no need for the plates to be sterilized after streaking? just like what that person did to the mouth of the tubes after dipping the inoculation loop

I would pour 25-35 mL in a 9cm plate. It depends on incubation times and the humidity. I find that plates tend to dry out and crack if poured too thin.

Thank u 😊

Thanks a lot, it is very clear!

Nice video. thanks. will it still be alright if i will no longer use a vacuum? and to just wait a little longer to allow the water sample to drain completely?

Where in the video did you put the different concentrations?

Wow simplest explanation ❤️

Why kelbsiella on CLED may appear whitish blue while it is LF all the time? Thanx

Please make a video on In-vitro DNA Ligation

Very nice clean and sterilized explanation sir. Thank you for the video...i was expecting grown plate too.

Video is clear 😊 ⭐⭐⭐⭐⭐