Membrane filtration method to count bacteria in water

Welcome to our suite of NUI Galway Microbiology teaching videos. We hope that they will guide you in performing your experiments in a scientific and safe manner.
In this video we demonstrate membrane filtration, a method used in testing the quality of water samples.
A river water sample is filtered through a membrane with a 0.45 µm pore size (smaller than typical bacteria) and bacteria are collected on the surface. The membrane filter is incubated on selective and differential media to grow and count the bacteria in the sample.

[@k4tchy]

Thank you! Im doing a senior project and this video is very helpful.

[@BlackZero880]

Very nice clean and sterilized explanation sir. Thank you for the video...i was expecting grown plate too.

[@chinyereglory367]

Very understandable, thank you sir

[@shaioomsyr5922]

I wished to see the result

[@sandyseven6028]

Thank u 😊

[@sajulgasheryl343]

Nice video. thanks. will it still be alright if i will no longer use a vacuum? and to just wait a little longer to allow the water sample to drain completely?

[@microbiologyteachingvideos6490]

Thanks. No, this is a standard method and a gravity approach would be affected by settling time or suspended solids that could impact bacterial counts so it's better to use a vacuum pump if possible.

[@rvanisree6971]

Video is clear 😊
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[@microbiologyteachingvideos6490]

😊

[@nahidborhani4431]

should be done in a ISO class 5 HOOD?

[@jmlqauqau1154]

Media or which nurite agar in plates..??

[@microbiologyteachingvideos6490]

Membrane lauryl sulfate agar - used to culture coliforms