I have two flow cytometry equipment in my possession. the first is the Calibur and the second the Accure. When I run the cells through the two cytometers, I get different graphs for the same cells. In calibur, the 1000 by 1000 dimension graph presents a more vertical behavior, while the accure, with the graphics in the same dimensions as your video, presents a plot similar to the one you presented. Why does this occur?
Thank you for your kind instruction !
I have two flow cytometry equipment in my possession.
the first is the Calibur and the second the Accure.
When I run the cells through the two cytometers, I get different graphs for the same cells. In calibur, the 1000 by 1000 dimension graph presents a more vertical behavior, while the accure, with the graphics in the same dimensions as your video, presents a plot similar to the one you presented. Why does this occur?
How to export to powerpoint...i didn't find any option?
Thanks for the video. Really good Do you have any for Annexin V apoptosis analysis?
Yes, I have, I will upload it as soon as possible.
Bigggg thanks to you.
Thanks
links are not working
Not too sure about using FL2-A vs FL2-H for doublet discrimination... Shame we cant really use FL2-A vs FL2-W on this machine
But, we can use FL2-A vs FL2-H for cell cycle, right?!
Can you please share cell cycle protocol ? I tried with some other protocols but my results are not like yours .
www.biotechandmed.info/2019/01/Cell-cycle-flow-cytometry.html
And did you use the cold 70% ethanol or normal temperature ethanol?? and how was the cell cycle profile?