Agarose Gel Electrophoresis!

Поделиться
HTML-код
  • Опубликовано: 7 сен 2024
  • Hello friends. This is Ashutosh K. Yadav, welcome to my you tube channel AkyBiotech. This video is about Agarose Gel Electrophoresis: Casting of gel, Sample loading, Running and Gel documentation. We amplified DNA fragment by SSR marker using Polymerase chain reaction (PCR) and separate PCR product using agarose gel electroscopes.
    Full Video of Agarose Gel Electrophoresis links:--
    Part 1: • Agarose Gel Electropho...
    Part 2: • Agarose Gel Electropho...
    Kindly follow on: Instagram: / akybiotech
    Like our Facebook Page: / akybiotech
    You can join our Telegram channel: t.me/akybiotech
    -----------------------------------------------------------------------------------------------------------------------------------------
    #AgaroseGelElectrophoresis
    #AgaroseGel
    #akybiotech
    #dnaelectrophoresis
    #Gelelectrophoresis
    Please like, share and subscribe.
    Thank u so much for watching have a great day.
    Background music
    www.bensound.com

Комментарии • 66

  • @bananamilk1980
    @bananamilk1980 9 месяцев назад +132

    After reading the chapter i watched this , it made thibgs even more clear and interesting ❤

  • @k..5413
    @k..5413 6 месяцев назад +62

    1)denaturation
    2)primer anealing
    3)primer extension

    • @LinkedBio45
      @LinkedBio45 4 месяца назад +5

      Ye pcr hai bhai
      This method is use before the electrophoresis

  • @sunildhinwa6584
    @sunildhinwa6584 Месяц назад +46

    Seep mam plus student attendance mark ❣️❣️

  • @TanishSharma-tg8qw
    @TanishSharma-tg8qw Месяц назад +34

    Who came here after seep maam shared the link????

    • @Futuredoctor-id4xi
      @Futuredoctor-id4xi Месяц назад +2

      Mee

    • @KASHYAPGUNJAN-gq5de
      @KASHYAPGUNJAN-gq5de Месяц назад

      but ye bright orange kyun nai hai

    • @chanchalsoni2752
      @chanchalsoni2752 Месяц назад +1

      ​@@KASHYAPGUNJAN-gq5deQki ye ethidium bromide or UV rays wala method nhi h.....ek or method hota h....probe method [ autoradiography ]......usme DNA ko probe(radioactive DNA or RNA) se treat krte h or X-rays expose krte h , fir grey coloured bands dikhte h..........whi use hua h yha shayad

    • @sunildhinwa6584
      @sunildhinwa6584 Месяц назад

      @@TanishSharma-tg8qw me also

    • @snehashree972
      @snehashree972 Месяц назад

      Me

  • @anjali-chauhan395
    @anjali-chauhan395 Месяц назад +8

    I am come here after seep mam shared link ❤😊

  • @sanjeevkumar-tf1ty
    @sanjeevkumar-tf1ty Год назад +48

    Really useful and nice video, pdf is also very good

  • @AaravChaugule-kw6gz
    @AaravChaugule-kw6gz Месяц назад +5

    Seep mam plus student❤

  • @chintanjain4638
    @chintanjain4638 Месяц назад +3

    SEEPIANS MARK UR ATTENDANCE :)

  • @mmgaming07860
    @mmgaming07860 29 дней назад +2

    Seep ma'am students here after sharing link by her😊😊😊

  • @vishnuvishwakarma_waytosucces
    @vishnuvishwakarma_waytosucces 10 месяцев назад +16

    Really it is interesting video... We can relate the given topics

  • @diyathakkar2152
    @diyathakkar2152 Год назад +11

    Really very useful ...
    Thank you ma'am 🤗

  • @proficientuikey859
    @proficientuikey859 2 года назад +6

    ayesi hi or videos chahiye mam

  • @RahulKumar-st9ys
    @RahulKumar-st9ys Год назад +10

    How will you get ur desired part of DNA for further processing

    • @AkyBiotech
      @AkyBiotech  Год назад +6

      Based on allele/product size, u will get the desired DNA..

  • @pikscooking1798
    @pikscooking1798 Год назад +16

    BIOTECHNOLOGY OF CLASS 12 😌

    • @incredulous-
      @incredulous- 10 месяцев назад +6

      Not just in, It will help you later too
      Even I am watching this, being a mbbs student

    • @pikscooking1798
      @pikscooking1798 10 месяцев назад +3

      @@incredulous- Yeahh !! One Of The Perfect Chaper Of Mine ! 💗🔥

    • @Biologyyt
      @Biologyyt 5 месяцев назад +1

      Y

  • @geetarthabharali2200
    @geetarthabharali2200 Год назад +4

    Very useful

  • @vedansh_anil
    @vedansh_anil 11 месяцев назад

    Mam please you can explain whole process and material required for this project

  • @nisha_1134
    @nisha_1134 Год назад +14

    But I have a question how will you find the desired gene for the elution process in the gel ?

    • @AkyBiotech
      @AkyBiotech  Год назад +10

      Based on product size u can elute desired gene...

    • @saturogojo5648
      @saturogojo5648 10 месяцев назад

      based on the density smaller fragments will move farther away and heavier will remain behind so you can differentiate easily

  • @ronakmaurya5227
    @ronakmaurya5227 Год назад +2

    Plz do the elution also

  • @alom___0.1
    @alom___0.1 9 месяцев назад +1

    How to confirm that which fragment of dna contains the desired gene segment

  • @ananonymousarmy
    @ananonymousarmy Месяц назад

    Wow even though ethidium bromide is a carcinogen it still has roles.

  • @720AIR
    @720AIR Месяц назад +1

    2024 😊👍

  • @Blood008
    @Blood008 10 месяцев назад +1

    I❤it mam I just wanted a detail video to learn

  • @bushraanjum5802
    @bushraanjum5802 3 года назад +1

    thank you mam

  • @natrajdancehub8885
    @natrajdancehub8885 7 месяцев назад

    Thank you

  • @mpsmss67
    @mpsmss67 Год назад

    Why gel background become bright white sometimes? what is the problem behind it?

  • @vishnukawade5021
    @vishnukawade5021 11 месяцев назад

    what is use of blotting using nitrocellulose or nylon

  • @lurjo3109
    @lurjo3109 Год назад +2

    Why is the place dirty

  • @MrHafizsajid786
    @MrHafizsajid786 Год назад

    Thankss

  • @anukriti_1983
    @anukriti_1983 8 месяцев назад

    Beautiful

  • @bhavya540
    @bhavya540 8 месяцев назад

    Amazing

  • @Unique_boy_abhi_9651
    @Unique_boy_abhi_9651 8 месяцев назад

    Ethidium bromide

  • @user-pi8ew3wk4k
    @user-pi8ew3wk4k Год назад

    You have not used Uv light

  • @neelkrishnadas4108
    @neelkrishnadas4108 8 месяцев назад +1

    Neet aspirants ❤ attendance please
    Concept chamkis ❤

  • @Ankit-cf5mn
    @Ankit-cf5mn 18 дней назад

    Nice. Humare college mai toh iska bhi jugaad tha. Jab aukat nahi hai toh pata nahi admissions kyu dete hai in Pune colleges. Band hone chaiye aise colleges which destroy students future.

  • @AdityaSharma-ei3ur
    @AdityaSharma-ei3ur Год назад +1

    Nice 🙂👍🙂👍🙂👍

  • @yekulanagakarthik1715
    @yekulanagakarthik1715 6 месяцев назад

    😍😍WOW

  • @starGirl-dl1rx
    @starGirl-dl1rx 3 месяца назад

    Is this PCR?

    • @AkyBiotech
      @AkyBiotech  3 месяца назад +1

      Post PCR...

    • @starGirl-dl1rx
      @starGirl-dl1rx 3 месяца назад

      @@AkyBiotech thank you for the swift reply 🙏

  • @soorajsooraj528
    @soorajsooraj528 Месяц назад

    Smitha pooru

  • @AlchemisacademybyUsazad
    @AlchemisacademybyUsazad 2 года назад

    Grand 👍

  • @sananawaz5862
    @sananawaz5862 2 года назад +2

    Its so fast...need detail video

    • @AkyBiotech
      @AkyBiotech  2 года назад +3

      U can find the full details video link in discription box below

  • @bharti0908
    @bharti0908 9 месяцев назад

    ❤❤❤

  • @shivamnaveen9948
    @shivamnaveen9948 Год назад

    Maam in which Institute you arr studying

  • @SHREYU_MEDICO.
    @SHREYU_MEDICO. 6 месяцев назад

    😊😊😊😊

  • @MayaGupta-ju1co
    @MayaGupta-ju1co 9 месяцев назад

    Hindi bolte toh aur v accha hota mam

  • @AnujKumar-qh7tx
    @AnujKumar-qh7tx 3 года назад

    🙏🙏🌼🌼💐🌼

  • @user-vn3ov2vl4h
    @user-vn3ov2vl4h 10 месяцев назад

    Thanks 😊 mam