Agarose Gel Electrophoresis
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- Опубликовано: 10 окт 2012
- For more information, visit www.bio-rad.com/yt/idea.
This video demonstrates how to load and run DNA samples on an agarose gel. Basic information about the charge of DNA and how it will run in an horizontal electrophoresis cell is explained.
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Hop Barış hocamdan geldik buradayız
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@DR.BİYOLOJİ HOCAMDAN SELAMLAR
Barış Hoca'dan gelenler
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Dr. Biyoloji 💐
Barış hocadan gelenlere sa
DR Biyoloji den gelenler
How many are there after studing biotechnology 12 class
@DoktorOnia nice
MSC microbio
Me too
Me
Nah , I'm here for college lab exam
Barış hocam bir tane 😊😊
Dr. Biyoloji Dı dım tı tıs 🥁
I just realized why I was doing this in lab because the instructor did not explain a thing! Thanks soo much! God bless 🙏🏻
Thankfully my instructor is a boss and covered all of this and used this to show the process, hope you get a better teacher next semester.
I'm interestingly interested in this
Barış hocam 🤟🤟
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Excellent description on GEL ELECTROPHORESIS...MANY THANKS FOR UPLOADING THIS LECTURE ❤️
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Came from "DR. BİYOLOJİ" chanel 🤙
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Dr Biyoloji🫡
1. siapkan sub sel mini
2. sejajarkan gel sehingga sumur paling dekat dengan elektrode negatif
3. tempatkan egl agarosa ke ruang gel
4. tambahkan buffer elektroforesis ke reservoir sampai reservoir tertutup buffer elektroforesis sedalam 2mm
5. tempatkan sampel sesuai urutan yang benar
6. tempatkan sampel ke dalam sumur dengan menggunakan mikropipet. jagalah pipet tetep tegak lurus terhadap lubang
7. pasang tutup ruang gel sesuai dengan elektrodanya (hitam ke hitam, merah ke merah)
8. sambungkan elektrode ke catu daya
9. nyalakan catu daya
10. atur tegangan konstan sebesar 100V
11. atur timer menjadi 60s
12. amati perubahan yang terjadi
Amazing...to see the DNA fragments...1. Smaller the fragment size ,the farther it moves. 2.Agarose is natural polymer extracted from Sea weeds. 3.Separated DNA fragments can be visualised after staining with ethidium bromide.
NCERT! 😂👌
Who the hell still uses ethidium bromide!??!? SMH...SYBR Safe has been around for like a couple of decades now...there's absolutely no need to keep using the HIGHLY carcinogenic EtBr...wow...
Lovely, I'm interestingly interested in this.
I just finished running the three practicals now, on center for molecular bioscience and biotechnology lab in SCH
Hi can i ask you some questions maybe in the future for academic purposes only. thank you. is it okay if i can contact you through email?
Watching it during quarantine 😭😭😝
haha
Omg i passed that semester and still corona didn’t ended
@@sondos8928 hahahaha I’m doing it for mine right now
@@sondos8928 "ended"
im sorry quarantine was a year ago-? holy shit-
How nostalgic. I did this as a practical exam when I was in college and completely ruined the agarose gel 😂😭😭. My prof got really mad.
Damn can't wait till I go to college...
What happened exactly? How does one ruin the agarose?
for the blanck I use the leader + load dye. for samples of dna you need load dye + dna + enzime + enzime buffer, I am missing something?
Barış Hoca sayesinde geldik
So exciting, this!
Thank you so much. I learned lessons but i never applied and i am still scared 😅
What should we do if there more bubble in red electrode?
Please make a video on sample preparation also
Thank you for this
very informative . thanks a lot.
Thanks
For the bands to be visible…. No need for ethidium bromide and uv light exposure?
instead of pushing the pipette all the way through when loading the wells, you could release pressure to suck the bubble back in. that way you avoid marked sample spilling out of the wells - not that it makes a huge difference, i just like to keep the buffer clean.
Or you could not push to the second stop
@@Ronnicus lol
I love it =) It's so interesting and beautiful at the same time =)
So are you :p
Very cool video, well done!
1:56 oh, that is so damn satisfying.
When he pippet sucked the entire sample I said fukkkk so perfect
Thank you.. Well explained
osmmm video! plzz upload more more dis kind of video so that it is to understand
thanks my dude
This is very nailed and good knowledge thanks 🙂
Very informative.
very well done video
how I can label the product of gel electrophoresis by which program if my gel image was taken
by JPA please kindly for you recommend me thanks
excellent videos
This was awesome
very Informative
Hello, any distributor of this instrumentation and consumable in Indonesia? Thank you
What do we need the buffer for ?
Is fast blast DNA stain as good as ethidium bromide,i mean can it practically replace ethidium bromide,are there any hazzards concerned with this stain as it is with ethidium bromide
+Neelam Singh,
In our University Biochemistry lab today, we used SYBR safe DNA Gel Stain. It is less mutagenic than ethidium bromide, and can be used under blue light just like ethidium bromide.
I would feel much more comfortable buying this solution from Thermofisher Scientific. You can buy the solution here, as well as see the tests used to validate its efficiency on this page, as well. (Notice how the page is padlocked when you follow the link.)
www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html
Very good information tq it is useful of my lesson .
When we did it we couldn't really find the wells and struggled for almost an hour and a half to find the wells.
really good
Nomesh sir PW show this in class 😎😎 rewatching this video after class ❤❤
Very understanding , thank you
Thank you so much!
I don’t need sybr dye for this step right?
That's my favourite experiment
Thanks. It’s very clear
Very Interesting 👍🙏
Why do we see more bubbles at the black electrode ?
that was great thank you
very very good
thanks .. its good to learn the exact technique.
Nomesh sir jindabad
Thank you!
Barış hocam yollarsahoop buradayız
Excellent thank you too much
fantastic
Good effort
Please how to prepare agarose gel and buffer to post the vedio
Which dye do you use? And do you add the dye into the centrifuge?
Ethirium Bromide as the movement indicator and Bromopropanal as the blue dye.
Thank you! This helped a lot! But hey, where's ethidium bromide and UV rays? And bright orange bands of DNA?
Yes.. Atlast we visualize clear bands by uv rays .. They didn't mentioned it
Harika bi sey
Please DO NOT stick your pipette tip in the well while loading,you might puncture it, not to mention the sample might hit the well and spurt outside the well.pour enough buffer so that your tip is immersed but is still right above the well and release sample slowly. The loading buffer in your sample, in this case the blue stuff, contains glycerol which is heavy and 'leads' your DNA safely in the well.
I do what I want
@@whatevervlogs9663 you can choose to learn or not. your call mate 🤷
Actually I'm curious to know how to load the sample.if I simply put the sample in the well doesn't it get mixed with buffer.or u directly put the sample inside the agarose??
@@srividhyanarayanan7337 your sample should already have buffer in it because you use the Loading Buffer LB I use a 1 microliter of 6x loading buffer (trisborate a couple of dyes and glycerol) and 5 microliters sample therefore your LB is diluted to 1x. If you are using 1x TBE or TAE the liquids in theory are close isotonically even though the actual samples vary. do not worry. the glycerol in your LB is heavy enough it will pull the sample into your well because gravity. Just practise a bit - put the tip above the well IN THE BUFFER and slowly release sample (no bubbles) you will see your sample-dye sink to the bottom of well. Maniatis protocols explain this nicely. Let me know if you need a recipe for LB .
@@papoutsothiki I don't think that's a thing, the pipette is supposed to puncture through the gel to allow the plasmid dna/ genomic dna to enter the electrophoresis casette
Thank sir, I got it❤❤❤
Its helpful ,thanks but please explain material reagents and their usage..
what can be used as buffer solution except for tbe and tae?
Water
Thanks
A Clear video..
i messed this up in lab. wasted 3 weeks preparing my cheek cells to see if i was a taster or not. smh.
It's very important.thanks.
What happens if I place samples in the wells first -- that is ahead of the buffer?
Sorry to reply a year later, but in case you or others are wondering, I do NOT recommend this. If the samples are already in the wells when you add the buffer, the movement of the fluid could cause the samples to spill out of the wells, leading to contamination
thanks helpful
De