Thanks. Nice video. Very helpful. We saw the platelets at 100x.. How do we determine the platelet count? What is the multiplication factor? Is it 15,000 or 20,000?
The multiplication factor or 'Fudge Factor' has to be determined by the manufacturer of the lens you are using because the field of view can be smaller or larger depending on the brand of ocular lens. In my example I used a factor of 20,000 to calculate the plot count. See my process explained here: drive.google.com/open?id=1ryd...
For WBC grading you can use a 50x oil objective if it’s available. Some places only have a 40x dry or 100x so depending on your facility or SOP you will use what is validated. Here I used a 50x oil for WBC. For platelet estimate and WBC estimate I used 100x objective.
I went back and looked at the cell at 9:59 and it looks to me to be a monocyte. The cytoplasm does look a bit dark (should look more like ground glass) but if I recall, this was an older slide we had in the lab and the video has discolored the cells a bit. It’s size and cytoplasm are more indicative of a monocyte than a lymph. Also the cytoplasm has less integrity when interacting with RBCs than a lymph does. Great question!
This Wright Stain. It was a 3 step process: 1. Ethanol about 30 sec 2. Eosin about 6 sec 3. Wright stain about 20 sec Rinse in deionized water and let dry.
This was helpful, thank you (:
really cool to look at the cell in this video. It would be helpful to mark the cells that you mention when you scroll left/right and up/down.
Left / right is a correct way but the learning purpose use up/down
thank you, this helps me with my study. again, thank you!
I’m glad it was helpful.
I miss teaching ....Use to teach a MLT program back then
Thank you. This was very helpful.
Great work bro. Very helpful.
Thank you very much .. I’m new Lab Tech & this is huge help 👍👍🙂
It was so helpful, thank you
Thanks. Nice video. Very helpful. We saw the platelets at 100x.. How do we determine the platelet count? What is the multiplication factor? Is it 15,000 or 20,000?
The multiplication factor or 'Fudge Factor' has to be determined by the manufacturer of the lens you are using because the field of view can be smaller or larger depending on the brand of ocular lens. In my example I used a factor of 20,000 to calculate the plot count. See my process explained here: drive.google.com/open?id=1ryd...
Hi thanks for this video. what objective do you use for the wbc estimate, platelet and morphology grading?
For WBC grading you can use a 50x oil objective if it’s available. Some places only have a 40x dry or 100x so depending on your facility or SOP you will use what is validated. Here I used a 50x oil for WBC.
For platelet estimate and WBC estimate I used 100x objective.
thank you vary mach these is vary nice vida
Thanks sir very helpful video
Really helpful video👍
Could you tell me what the WBC at 9:59 is? The cytoplasm makes me want to say lymphocyte but the nucleus does not
I went back and looked at the cell at 9:59 and it looks to me to be a monocyte. The cytoplasm does look a bit dark (should look more like ground glass) but if I recall, this was an older slide we had in the lab and the video has discolored the cells a bit. It’s size and cytoplasm are more indicative of a monocyte than a lymph. Also the cytoplasm has less integrity when interacting with RBCs than a lymph does. Great question!
Nice.I am from Chittagong, Bangladesh.
Great Sir
Thanks ✍️
thank you!!!!
❤❤❤❤
Which stain is this ?
This Wright Stain. It was a 3 step process:
1. Ethanol about 30 sec
2. Eosin about 6 sec
3. Wright stain about 20 sec
Rinse in deionized water and let dry.
@@fenen23 thanks sir.. i do leishman but not looks like this, so i asked.. thank u so much
very helpful
Hi maam abi
It's too fast 👎👎👎👎