🔬 001 - How do you use a microscope?
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- Опубликовано: 10 янв 2018
- This is a complete tutorial on how to use a microscope.
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I receive many questions on which microscope one should buy. I recommend the following models for amateur microscopy (Affiliate Links to Amazon):
Children’s/Introductory - Swift or National Optical SS110: amzn.to/2WdS4PX
For Amateurs - Swift SW380T (with phototube) or Swift SW380B: amzn.to/3fu7aIY
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Fantastic ... I purchased two microscopes about three years ago ... one compound, and one dissecting microscope.
Sad to say that I have never used either of the :-( not because I don’t want to, but because I haven’t had tuition on them and I didn’t want to break or damage them.
I purchased them for my own pleasure/hobby as I have always been fascinated by the natural world and looking at parts of plants, insects (not that I would kill an insect to look at, but plenty of dead insects about).
A friend did say he would come round and show me how to use both of the microscopes, as he works for the local museum, but he never came round to help me.
I purchased all the equipment to go with the microscopes, slides, covers, dissecting kit, dishes, trays, pipits, adhesive, all the slide making equipment to.
However, after seeing this RUclips channel, I am more inspired now, and more confident to go forward by myself, and look into this fascinating world.
Many thanks for these videos, absolutely fascinating and amazing, thank you :-)
6yrs later.. I hope you unpacked at least one to see how it looks.. :D
I'm so glad that I found this channel. So excited to learn and try more! Just starting! Thank you!
True
This is the best video clip about learning how to use microscope. I watched many clips, but still could not figure out completely how to use it, cause i study online. Many thanks for your best effort to upload this beautiful and useful video clip. xoxoxo
I just bought my third microscope! It is the first one that is something of a modern design, and though it is used, it is hopefully good enough to get some decent photos! Thank you for the great videos! This one is WAY better than the one I saw from a Texas community college! You tell how to USE the scope, they were telling how to pick it up, cover and uncover, and "clean" it.
Thank you for this great tutorial! It really made me want to buy a microscope.
Thank you help me understand the condenser and iris operation
Great tutorial!
Thank you very much for the video. I was clueless.
I´m so glad to found your video tutorials over here!It was a big help!Thanks for this!:)
I just got a Olympus CH40,like yours,so please one question master!:)
In the Olympus CH40 menue they say it´s the PM-10M3 for photography you need.
In the moment I just found an Olympus PM-10AK Shutter Block with Camera Mount + Controller ,
I´m asking myself,if this one could fit to the CH40 too?Already got the OM-mount photomicro adapter L and the adapter to
my Canon 5d Mark2. Or do I really need a triocular head for this?
Thanks!
Love & Peace
Nice video. Thanks!
good video bro thx ..
Thanks
Great! I'm a relative beginner and would like to pick up a set of top-quality prepared slides of various objects. I don't have any preference necessarily for exact type. I'd just like a good cross-section of interesting objects. I would appreciate any recommendations you might have. Perhaps you already have a vid on this that I haven't seen yet. Many thanks.
I do not have specific recommendations, but in the amazon shop I included links to a range of different permanent slides:
www.amazon.com/shop/microbehunter
Do you keep your glasses on or take them off when viewing?
I'm a student medical technologist but I'm finding it hard to position my eyes to see one specimen in the binocular eyepiece.... Can you teach me with the necessary ways?
Can I ask why does the condenser move up and down? Do you always use it in the highest position? Should the iris always be fully open when focusing?
Condenser should be up always. Can be moved down to reach screws to detach it. When focusing condenser should be first closed so that it is easier to find the focus (depth of field and contrast is highest when closed). later it can be opened and then you can continue to focus with the fine focus knob
@@Microbehunter Thank You! I love your videos!
I am having a really hard time getting an image with the 40 x
One point Oliver. I was told always to revolve the objective turret in one direction to avoid wear. I noticed you turned the turret back from the 40x to the 4x. I would have continued in the same direction to the 4x. Do you think that it matters which method you use?
I have never heard this. The revolving nosepiece has a ball-bearing, so this should not play a role. Rotating back from 100x to 40x is dangerous, if there was immersion oil on the slide, and this can cause contamination of the 40x objective. But this has nothing to do with wear.
@Microbehunter Thanks Oliver. I think it was probably on the Yahoo Microscope Group more than 10 years back. At that time it had many members who were very besotted with old Zeiss and Leitz stuff to the exclusion of almost everything else. Things often became so argumentative that a few members broke away and formed another group, Amateur Microscopy.
I like it
Hi, I wanted to know what do you think about the microscope Optika B192. Felicitations for the beautiful channel!
My recommendation is always the same: if you are already willing to spend a bit more on a microscope, get one with a phototube. Even if you might not want to do photography now, you might want to do it in the future, and a trinocular microscope is more convenient to use for photography. Example: Optika B-193PL
@@Microbehunter thanks
Hello! There is a problem regarding my microscope... Basically earlier the coarse adjustment was working, and the microscope was going up and down accordingly... But now it's not working ( I think after I took the objective lens away from the slide by using the coarse adjustment, after sometime it got out of control) plz help in resolving the issue, it's a sincere request 🙏🙏🙏
If the stage always goes down automatically, then you need to adjust the tension. More information needed on what is not working.
what accent do you have? I was thinking German or French
german
Austrian
what is the cost of this microscopic
This microscope is not made anymore. Search for Olympus CH40 to find out second hand prices on Ebay. Several hundred EUR.
Wow, he’s made over 200 videos now
You mentioned and also did one thing that made me jump in my seat -here:
ruclips.net/video/vlwtTLKWYSY/видео.html
That is a big NO!
Never ever turn the revolverhead while looking through the ocular of your microscope!
Always! Always look at the revolverhead as you ever so slowly turn the next objective into locked position!!
ALWAYS!
This is especially important, when you look at slides you have prepared on your own, ao when you look at permanent-slides for the first time!
If the speciment is more that a few cells thick, then you could scrape the objective against the slide, as you turn the revolverhead!!!
For that reason -Always observe the space between the 'incoming' objective and the coverglass on the slide, and not just trust 'that it fits' and glare through the ocular, while turning the revolverhead!
Obs! This is ABSOLUTELY *V I T A L* if you use a microscope older than 60', because even though some of the finest optical microscopes are from that time, the geometry between board and revolverhead is NOT synchronised!
Here you MUST turn the coarse focus to increase the distance between board and objectives BEFORE you turn the revolverhead!
Also you never focus by raising the table!
Always lower the table when you focus!
Hello, thank you for your comment. A few clarifications here.
>>Never ever turn the revolverhead while looking through the ocular of your microscope! Always! Always look at the revolverhead as you ever so slowly turn the next objective into locked position!!
The video shows what the camera has seen, and not what I have seen. While changing the objective I was looking both at the LCD of the camera as well as the revolver. For objectives that are parfocal it is possible to change the magnification without looking. If you do contact the cover glass, then you will immediately see this (in wet mounts, not permanent mounts) as the specimen moves. The distance is so small, that it is difficult to see anyway. I have to be able to trust the setup of the microscope that things like this are not going to happen. I am also less worried about making contact while rotating the next higher objective into position. The objective swings in from the side and if the distance is small then the metal of the objective will make contact first. I think that it is much more problematic if the permanent specimen is thick, and when one focuses on a lower part and then contacts the cover glass and then additionally moves the slide after contact has been made. This might indeed introduce scratches.
I disagree that one should increase distance when switching to the next higher magnification with parfocal objectives. You will lose the focus and then one hast to move the stage again up, exactly that what one wants to prevent. I understand that one might have to increase distance, especially if one mixes short barrel and long barrel objectives, but I think that this is something that one should avoid anyway and people who are experienced will be able to handle this as well.
About focusing by raising the stage: one should not use the coarse focus for raising the stage, but using the fine focus, is normal practice. One should not spin the fine focus many times, if one has lost the focus, bur restart with the 4x objective.
From my experience, the mistakes that many beginners make are of a different nature anyway: they use immersion oil with non-oil objectives or rotate the objective into mounting medium (used to much of it and did not wait for it to dry) or they confuse mounting medium and immersion oil (despite proper instruction). For this reason, I suggest that for educational scopes one should use the cheapest available objectives. They are almost disposables. Hate to say it.
first vidio .... starting from here