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here is a question! if the methylation is saving the prokaryotic DNA to be cut be by the restriction endonuclease, how can we cut some DNA of interest of a prokaryote by a restriction enyzme? Will the enzyme be able to cut the DNA of interest in the presence of methylation?
1) Mam can alkaline phoshphate enzyme remove OH also from the genetic material or does it remove poshphate only? 2) does methyl group gets attached to the N2 bases of host's genetic material only?
Mam I saw many online lecture But your teaching is so beautiful You lecture take less time and covered all concept with better understanding Thanks ma'am your teaching help so... Thank you thank you
Because MS can recognize its cells DNA strind( bachterial ).it works for endonuclease by diffrentiat its cells dna by methylates to it. Thats how endonuclease cuts foreign DNA .
*Hello Ma'am, Name the Four Nuclease Enzymes Each For Cut the Sticky End At 5prime And 3prime Ends And Four Enzymes That Cut At The Blunt End With Their Recognition Site*
Sry for interruptions 😊... As I know... Vectors have multiple restriction sites but an endonuclease is confined to cut the chromosomal DNA only at a specific site...( like either blunt end or a staggered cut) ... Coz for this unifying feature , a particular endonuclease is allowed to cut both the gene of interest and vector...
Visit www.neelabakoretutorials.in for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
Mam ek chota sa correction hoga shayad..wahan the roman no.indicates the order in which the enzyme was isolated frm the strain of bacteria..nd HindII first discoverd not first isolated hoga
Mam but why can't the methylated group be attached to the A and T of foreign organism while the endonuclease can't differentiate between self and non-self
I think you switched between first isolated and first discovered...because it’s clearly written in ncert that Roman numbers are based on order of isolation
@@MuhammadYaseen-dd1uw NO... as hydrogen bonds are present between two strands of dna ..they are not broken...because..here we are seperating the G and C ...which are present complementarily in the opposite strands...as the dna is seperating at the same place in both the strands...hydrogen bonds need not to be broken...
@@KurapatiKvsnRaju as hydrogen bonds are present between two strands of dna ..they are not broken...because..here we are seperating the G and C ...which are present complementarily in the opposite strands...as the dna is seperating at the same place in both the strands...hydrogen bonds need not to be broken...
even though u r speaking english im feeling that as u r speaking my mother tongue.u r telling in that easy wayy.... hatsoff 2 u madam jeeeee.....
Visit www.neelabakoretutorials.in
for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
Mam your teaching style is awesome.
Thank you very much Madam you make our basic ,stay bless
The real teacher on you tube for biology
Your effort is amazing
For all of us premedicos.
Mame way of teaching is really overwhelming and elegant .you clear the concepts like a crystal
Thanks for teaching the most difficult chapter...in such a simple way....
Mam ji its supr sai bhi upr really too much easy to undrstand 😇😇😇
U r explaination is really awesome Ma'am..... Thanks a lot.... It's really helpful...
Thanks from side of ma'am
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THANKS MAM
THIS VIDEO MADE MY CONCEPTS STRONG
Mam u r very good teacher I have ever seen
the way u draw diagramss do beautifully and in a very understandable way. YOU ARE LITERALLY AN ANGEL SENT FROM ABOVE FOR NEET ASPIRANTS😭😭
I am very grateful to you ma'am ❤🙏...excellent teaching ..everything is clear now
thank u so much mam....ur teaching is awesome ...hoping more n more videos from u....loved ur teaching way 😍😍😍😍☺😊😊
here is a question! if the methylation is saving the prokaryotic DNA to be cut be by the restriction endonuclease, how can we cut some DNA of interest of a prokaryote by a restriction enyzme? Will the enzyme be able to cut the DNA of interest in the presence of methylation?
You are Excellent mam..Thank you so much for enhancing our knowledge by making such online platform.
Amazing mam. I hope you would be awarded for best teaching. Really very well understood. Thank you.
ur lectures are awsome mam... thanks a lot mam..
THINK WITHOUT InK
really mam i wish .. u were my teacher 🤗🤗🤗
Hello ma’am!
Why is it easier to add insert on sticky end than on blunt end?
@@kalendersheriff8744 oh ok thanks for the info
@@kalendersheriff8744 thanks a lot
A clear explanation comes only when person himself has clear concepts... u r da best exmpl mam...
The revolutionary teacher🤗...neela mam🙏
1) Mam can alkaline phoshphate enzyme remove OH also from the genetic material or does it remove poshphate only?
2) does methyl group gets attached to the N2 bases of host's genetic material only?
Thanku
Thank you so much mam ❤️❤️
Thankew mam fr helping mee sty always blessed 🤗😊
I am also a biology teacher yet you are my mentor ma'am...i heartily salute you 🙏..hope one day i would also be helpful as you are for the kiddoss
Visit www.neelabakoretutorials.in
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mam bacterial DNA have Uracil in place of Thymine mam
Thank you very much mam
😊😊😊awesome teaching ma'am 👌👌👌👏👏
Thank you Neela mam
Mam if that lysozyme enzyme kills the bacteria then could we isolate the DNA???
The best teacher on you tube for bio.
13 Dec 2020
Maam u r an angel in this lockdown period to all kids of class 12
Mam I saw many online lecture
But your teaching is so beautiful
You lecture take less time and covered all concept with better understanding
Thanks ma'am your teaching help so...
Thank you thank you
nice teaching....
Thank you mam for making the concepts more clear.
I have a doubt about, in sticky cut two types of bond are broken and in blunt cut only one type of bond phosphodiester bond broken how?
Best teacher 😘😘😘
Mam, Why methylating system do. not add methyl group in the virus genetic material? Is it differentiated by MS?
Same doubt
Because MS can recognize its cells DNA strind( bachterial ).it works for endonuclease by diffrentiat its cells dna by methylates to it. Thats how endonuclease cuts foreign DNA .
In short ,MS have ability to recognize its cell's DNA.
@ANUSHKA MISHRA thank you so much.... Anushka mishra
@@surendrasharma5677 and u too thanks
mam use orange marker instrede of red marker orange colour looks clear then red colour please nice teaching u are having mam
tnq maam...u r vdo hlps me a lot
thanx mam MaY ALLAH give u long life full of hapiness....Ameen
*Hello Ma'am, Name the Four Nuclease Enzymes Each For Cut the Sticky End At 5prime And 3prime Ends And Four Enzymes That Cut At The Blunt End With Their Recognition Site*
Does each endonuclease have a unique property of cutting at different sites and how does this done?
Kindly clarify my doubt, ma'am.
Sry for interruptions 😊... As I know... Vectors have multiple restriction sites but an endonuclease is confined to cut the chromosomal DNA only at a specific site...( like either blunt end or a staggered cut) ... Coz for this unifying feature , a particular endonuclease is allowed to cut both the gene of interest and vector...
Just watching 👀 lectures...feels good 👍
Thanks ma'am ❣
Thank you Mam for teaching things in an easy way
Your way of teaching is simply fantastic
Amazing. .. lecture maam...soo helpfull. ... ! 😊
Apkai padha bilkul apki andaz se match karta hai ❤
ma'am ur teaching is excellent....thank you for providing such amazing videos!!
Best way of teaching in the most simple way
THANK YOU MAM ❤️
PERFECT EXPLANATION !!
THANKS MAM
Mam so much thanks ...bhot ache se aap ne mere concpet clear kar diye ...so much thanks mam..........
Best biotechnology teacher you clarify all my doubts of this topic 🥺 we love you Ma'am ❤️
Visit www.neelabakoretutorials.in
for a well planned road map to help students score 360 marks in NEET Biology. Please share with those appearing for NEET.
Thanku very much mam ur teaching skills are ossum I was very much confused with this topic but after watching ur session I got a clear picture of it
Same here
Mam I have a doubt
How do you giving the code name for E. coli and haemophilous influenzae?
Mam ek chota sa correction hoga shayad..wahan the roman no.indicates the order in which the enzyme was isolated frm the strain of bacteria..nd HindII first discoverd not first isolated hoga
In NCERT, it is written that the Roman numbers indicate the order in which the enzymes were "Isolated". Please check.
Amazing lecture mam.... Thank u
all lectures are very good
Thank you ! :)
excellent mam.......
Mam, how would we get to know about the discovery of bacteria???? 🤔🤔🤔🤔🤔🤔
Plzz tell............if some other one know the answer guyz!!!!
mam i have a doubt on pallin drop u gave a example on gaattc by using any word we have to use the same method?????
Mam how will endonuclease correctly adds the methylating group to its own dna it can even add it to phage dna right ?
endonuclease does not add methyl grp, methylating agent adds methyl group
Mam but why can't the methylated group be attached to the A and T of foreign organism while the endonuclease can't differentiate between self and non-self
GREAT JOB mAM THANK U SOOO MUCH
your class is soo super and soo understanding one ...thank you soo much mam
Thank you mam .
Good teaching
ur teaching is awsm......,mam
Thank you maam
I think you switched between first isolated and first discovered...because it’s clearly written in ncert that Roman numbers are based on order of isolation
Too gr8 teaching madam jiii🙏🙏🙏🙏👍
In second case of palidrome sequence, why hydrogen bonds are not broken. Pls reply
Mam is gonna wrong in that case there is hydrogen bond between c and g which is cutter while the line shows phosphodiester bond
Can you plz explain it mam...??Even btwn G nd C there exists hydrogen bonds right??
@@MuhammadYaseen-dd1uw
NO... as hydrogen bonds are present between two strands of dna ..they are not broken...because..here we are seperating the G and C ...which are present complementarily in the opposite strands...as the dna is seperating at the same place in both the strands...hydrogen bonds need not to be broken...
@@KurapatiKvsnRaju as hydrogen bonds are present between two strands of dna ..they are not broken...because..here we are seperating the G and C ...which are present complementarily in the opposite strands...as the dna is seperating at the same place in both the strands...hydrogen bonds need not to be broken...
Thank you mam for this amazing session
Mam I have a question - is there a difference in endonuclease and restriction nucleases
Mam you're too good
Thanks mam
Thank you so much for these awesome videos!
I have a question, at 8:00 is there a mechanism that allows the cell recognize which A-T to methylate?
Restriction endonuclease have Restriction-Modification system.
thank-you mam your lectures are osm
mam your teaching is just awesome..can understand concepts very easily...btw mam can you tell me how we dissolve the nuclear membrane
THANK YOU MAM
Thnku mam ur teaching style is very nyc it helped me very much to clear the concepts thnks a lot mam☺
Thanks mam for concept
What does strain mean?..mam u said RY 13 is "strain" of Eschericha coli.
Great lesson ❤️
Inspired from you mam....
mam you teaches very well
Very good videos
your classes r indeed helpful
Superb explanation👌👌mam
Great teacher . Thumbs up !
Mam how is pdc enzyme is different from other enzyme
Tq so much mam😃
Are these lectures for Neet aspirants mam?
Thanks madam 👍
Excellent work
Mem what will happen if the methyl group is added to any of the two bases ?
Mem I can't understand this point plzzz tell me.....