Spore cleanup
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- Опубликовано: 5 окт 2024
- Mushroom Cult demonstrates rescuing germinated chestnut spores from an agar plate that has sporulating mold contamination. Why? What if this was your best plate from a rare spore print you only had access to one time. you better be ready. Practice when it doesn't matter so you are ready when it does.
Experimental Comentarty Contest!
Send your audio commentaries to mushroomcultpro@gmail.com
The best commentary will be added to the demonstration section of the video. Must be in mp3 format and no longer than 4 minutes and 20 seconds.
Submissions are due by January 25th
Get critical Myco-Ninjas!
Move the contaminated lid once, slowly. Taking it on and off is fanning the dish.
'fanning the dish' is this CIA cryptic messaging or smt
That spray bottle would fit in the SAB much better than the bottle that came with the yellow spray top! Thanks for the tip!
glad to help
Very nice video, hope to see you post more soon. I would follow your process throughout the growth of your plates, grain, substrate, etc!
I am working on following the process of collecting a wild mushroom, cloning, cleaning and so on. It will take some time but it's on the way.
This is the perfect video I was looking for thank you so much!
Glad it was helpful! let me know if you have questions
Use an SAB, don't say you don't need one. You need one if you don't have a flowhood, your bathroom is not sufficient. Make it super clear. There is enough misinformation out there without new peeps thinking a bathroom is the way to go. This is my biggest gripe heh.
thanks for your comment. I agree. Im working on a series for best practices. I am planing to be mush more clear on the cointent. I want to be a trustworthy resource. I am also wanting to push boundaries. many people in this community are stuck in a ritualistic and dogmatic devotion to a specific TEK without thinking about why.
A cheap 3x3 grow tent is exponentially better than a bathroom. I use a tent, SAB and a UV sterilizer for agar work.
Bathrooms usually the best place to grow mold to , wet damp conditions , not a place to do this work at all.
Well that was easy. Thanks for the info.
Glad it helped
The description is my nightmare scenario. This is why I keep two slants of all my best cultures, in a fridge.
exactly! I recommend throwing this away and not following my example. i just wanted to demonstrate the process.
I like your style.. you a good person
I appreciate that!
Thanks for the video, great info and straight to the point 👍. I'm subbed. For the first time did 12 no pour agar 3 weeks ago, in one jar there is a tiny miniscule black speck. To me it doesn't look like any of the contamination I've seen in videos. I'll keep an 👁 on it. Do you have any idea what it could be? It wasn't there in beginning, and I don't c it growing.
has the speck grown? it could just be a piece of black material
@@mushroomcult hi thx for your response. No the speck has not grown for a week sinds post. U think it's benign? Have no idea where it came from, I did a spore rig to no pour agar. Could it be a small cluster of spore?🍄🕉
I also noticed you’re not taking from the leading edge
great observation. thanks for your comment!
A tip for the next video, ur voice is a little bit too lound, lower it next time :)
I just noticed you don’t really do air filter or SAB do you normally do it like this or just for the video?
Looking to start agar plates but if I have them have some much equipment may just stick to injections even if slower lol
Interesting stuff.
I’ve tried save a few agar plates it never worked for me….probably beyond saving and contam had already spread extensively
Yah. This example is past when I would normally bother but it is good practice for when you have an emergency
@@mushroomcult One time I got in some agar dishes and 1 of the lids was cracked, I decided just to try it anyway for the hell of it I taped the crack with some package tape, and put some mycelium on it. Next day I noticed a bit of mold had showed up right under where the crack had been, so I opened it back up in still air box and cut out the mold spot then carefully wiped the rest of the agar with a paper towel I sprayed with some alcohol. It worked and no more mold showed up.
Maybe write your sharpie notes on the plate NOT the cover. Small detail, but sometimes stuff happens. Don't like the bathroom non still box, non flow hood, etc...MT, ASCP
what do you think of this lab setup?
ruclips.net/video/KmPHX1ZH-L0/видео.html
There are spores on the floor in the flat i rent.
Two days after cleaning they come back all the time.
Look like dust but it 'isn't.
exactly. a still air box is very helpful to keep your work clean
I just chuck mine at the local trash pandas
I have to say it is quite delicious
@@mushroomcult they can have a lil agar as a treat lol
Why don’t you flip the fish you’re xferring from? Also you shouldn’t have to open the dishes youre inoculating As far as you are, I like to only open one side of the dish. Just enough to get the wedge in. You should wipe your dish with alcohol after you pull your parafilm. I get what you’re saying about being gentle with the dish but it’s honestly not that big of a deal, I’ve dropped dishes with trich in them and still was able to clean them up no problem.
Are you saying you would do the transfer from an upside down dish? I'll need to try this and see how it feels. I like your comments about the fine details. ill watch the video again and see if i see whet you are seeing. Thanks a lot!
Dish, not fish lol. But yes, your multi spore dish should be upside down imo, otherwise it’s ripe for more contams on your clean culture you’re about to xfer.
@@zebraney5247 i was told that the reason we flip lids dishes is to avoid condensation
After pouring and sealing agar plates, how long should I wait before starting work with them? Would it be sensible to wait 3-4 days to see if any contamination from the agar technique is present?
that would be a good practice. as you get more secure in your technique you can use them as soon as they cool but set a couple to the side as blank controls.
I know nothing about mycology so don’t get frustrated at my ignorance but could you drop a small amount of bleach or alcohol just on the bacteria to kill it or would that kill the mycelia too?
It will probably kill both. This wouldn't be a good practice even if it works. The idea is to move the cleanest mycelium away from contamination. Antibiotics can be added to the Agar plates that can accomplish what you are thinking but it needs to be added before the plates are inoculated. Does that answer your question?
@@mushroomcult one more question, I lost one of my weights on my pressure canner and I can only get 10 pounds or pressure for my grain spawn jars, can I still get proper sterilization?
@@jamegumb6625 you need to run it maybe 25 - 30 % longer. You could also put a wet towel over the weight to hold more pressure if you are able to monitor it.
@@jamegumb6625 or tape a few coins on it till it wiggkes at the right pressure :)
I hate aspergillius ... It grows so fast!
it has its uses. great for soil health.
your videos audio is always so quiet...
I only want to speak to people who listen carefully.
@@mushroomcult 🤣💯
So you used the plate you were cooling the blade down in for the final inoculation and you didn't flame sterilize the blade use for the final inoculation?
i'll need to watch that again but i sterilize the blade, cool it in a clean plate before making a transfer. is that what you saw?
@@mushroomcult no, watch it from 7:00. You pull a new blade from it's packet but never flame sterilize it before using it. And it appears you use it on the same plate you were using to cool down previous blades.
@@Trenscendent why would you flame sterilize a brand new blade
@@ClaydogJr because brand new blades aren't sterile, duh
@@Trenscendent that’s a surgical blade they come sterile
do it over the oven then
What do you mean by that?
hi, beginner here. very similar situation. could u explain why it's not just easier to go with a Qtip and some H202 and clean up the mold ? I really like to save my clean plates as they are a pain to make. I have a cat (litter box), mold on some walls and an indoor grow op (mildew, botrytis - these things happen) so if I do what u did I'm risking the same thing happening. like I said tho, I'm only a beginner trying to see what works best for my situation. thanx
trying to remove mold will cause spores to spread all over the plate and in the air. you always want to move the clean mycelium away from the contamination on to an new plate. check out my video on the no pour agar technique. it will help you make agar plates without the hassle. does this help?
@@mushroomcult I've actually seen the video you recommended. instead of using tiny jars (which I don't have) can I pour the agar on contaminated petri dishes instead, put the lids on the dishes and PC the heck out of them ? I think theres no problem, my fear is the water/humidity go inside the plates. you know yourself these lids aren't very snug
sooooo... did you save the chestnut culture? Give us an update.
Yes the final video shows the whole process. ruclips.net/video/ULtGT04Xv_Q/видео.html The goal of this video was to create a demonstration for our students to reference after class so they could get their minds around what they need for cleaning up any cultures that they made in class with us. Once we had clean cultures the goal for this video was met so we didn't continue on to fruiting it.
I have a sab with air filter attached to it tht blows clean air continuously...cn I use this setup to clean my plates.
If I do so...won't the bacterial spores of contaminated plates revolve inside my sab.
Ur solution n knowledge is highly appreciated.
Great question. A still air box should not have moving air. The point is for the air to be still. A laminar flow hood is designed with a special filter that produces a laminar air flow. Most filters produce turbulent flow and are not useful for this. Also if you have mold you do not want to have moving air when the plate is open because it will blow spores. Does that answer your question?
@@mushroomcult ok...but my air purifier also has special carbon urethane filter n hepa filter which cleans 99.99 % contam.so I use it for agar n lc inoculation.i hv recently used this setup for plates n lc.will update in a week.
Ur opinion on this.
As far as contaminated plate is concerned...u r right sab without purifier sounds best to clean it.Running the purifier 15 min before cleaning n then turning it off during plate cleaning should give better outcomes I guess.
Pls enlighten me with ur knowledge on above 2 ques.
@@GodisGreat...89 yes. Turning off the fan if working with mold is a great idea. The HEPA filtration is good but not all filters are designed to have laminar flow. If you put a laminar flow filter in a box it can become turbulent when it hits the walls. A proper filter has a pressure chamber before the filter called a plenum that pushes air evenly through all parts of the filter.