RESTRICTION ENZYMES

For more information:
www.7activestud...
[e-mail]
www.7activemedi...
[e-mail]
www.sciencetuts...
[e-mail]
Contact: [phone],
040-64501777 / 65864777
7 Active Technology Solutions Pvt.Ltd. is an educational 3D digital content provider for K-12. We also customise the content as per your requirement for companies platform providers colleges etc . 7 Active driving force "The Joy of Happy Learning" -- is what makes difference from other digital content providers. We consider Student needs, Lecturer needs and College needs in designing the 3D & 2D Animated Video Lectures. We are carrying a huge 3D Digital Library ready to use.
RESTRICTION ENZYMES:In the year 1963, the two enzymes responsible for restricting the growth of bacteriophage in E.coli were isolated. One of these added methyl groups to DNA while the othercut DNA .The later was called restriction endonuclese.Restriction enzymes belong to a larger class of enzymes called nucleases. These are of 2 kinds'- exonucleases and endonucleases. Exonucleases remove nucleotides from the ends of the DNAwhereas endonucleases make cut at specific positions within the DNA.Restriction endonuclease always cut DNA molecules at aparticular point by recognizing a specific sequence of six base pairs.This specific base sequence is known as recognition sequences. More than 900 restriction enzymes have been isolated from 230 strains of bacteria each of which recognize different recognition sequences.
E.g.Restriction enzymes ECoRI.
E represents, the genus name Escherichia.
Co represents species name Escherichia coli.
These 3 letters are written italics.
R-Name of the strain.
Roman numbers following the names indicate the order in which the enzymes were isolated from that strain of bacteria.
Each restriction endonuclease functions by inspecting the length of DNA sequence &recognizes a specific palindromic nucleotidesequences in DNA Palindrome means A group of letter that from same words when read both backward & forward
E.g. 51 → 31direction
31→51 direction
51- GAATTC -- 31
31 -- CTTAAG -- 51

[@Astroplhile]

Perfect NCERT lines.....Good video and very informative....

[@gabriellobo3456]

Thank-you 7activestudio. This video was very helpful. Just a tip for next time; speak at a slightly faster, more uniform pace. Other than that, the content was top notch.

[@blockhet]

1.5 Speed and this sounds like a actual human.

[@jonathanaugustin647]

It actually doesn't because he keeps changing his pace.

[@miriri5329]

lol i tried and it's true

[@almaamaro5896]

blockhet g

[@greenheadmetalchips6669]

What a sad world we live in

[@godhelpme8977]

Condom

[@caleigh7575]

*hears voice* “oh hell no”

[@texemplarstudios5481]

Why can't the recombinant vector molecule be created unless the vector and source DNA is cut with the same restriction enzyme?

[@chungus816]

Because restriction enzymes are specific to a certain recognition sequence, meaning if you were to use a different restriction enzyme the resulting molecule would be different.

[@ashleshapoo_347]

Thank you bro very nice teaching love you 😘

[@northvanman1283]

He sounds like the guy from that SouthPark episode where they all go to the planetarium.....but the the astronomer calls it the 'Plane____arium' because he can't say T's

[@pramodarora1979]

very well explained as according to NCERT ..

[@minyongminyong6054]

Very few explanation... He is just reading as written in NCERT .

[@rakeshsreeramkr1675]

Yesss

[@curvinmathebula8232]

I couldn't hear anything because of that very loud beautiful music in the background but then then the parts that I could hear were fine

[@Career_Boost_Hub]

Thank you sir

[@7activestudio]

Welcome

[@shireentabassum6271]

Just awesome!!!!

[@sehrishkhalid126]

Watch this at 1.5x

[@saminaibrahim6837]

Very well explained

[@iboodzZ]

good video but the speaker is too slow!

[@7activestudio]

Now You can Access 120+ hours of digital content for free in APP
play.google.com/store/search?q=sciencetuts

[@MrAndres9545]

once again, too slow

[@wazidali2475]

عبدالله المزيد yes u say write

[@gamearena4259]

Thanks

[@MUHAMMADISMAIL-f7d]

perfect

[@nazmulsscience599]

so very helpful

[@7activestudio]

Now You can Access 120+ hours of digital content for free in APP
play.google.com/store/search?q=sciencetuts

[@maxmilian8433]

70% percent of humans hear this voice before death

[@jsannie56]

lmfaoooooo

[@kotireddyannapareddy7998]

Very good presentation

[@JayaLakshmi-ph9lm]

Superb vedio

[@ameersaifi8097]

perfect explanation and animation loved it !

[@pateldevang7486]

Its not dash its call 5 prime or 3 prime

[@manavichopra9085]

Very helpful! :)

[@7activestudio]

Now You can Access 120+ hours of digital content for free in APP
play.google.com/store/search?q=sciencetuts

[@vaishnasunil6566]

in stagered cut, where the enzyme cut the DNA

[@freedomtv1505]

GOD bless

[@vidyabijo6925]

excellent!!!

[@7activestudio]

Now You can Access 120+ hours of digital content for free in APP
play.google.com/store/search?q=sciencetuts

[@kamaleshwaran9330]

Nice video

[@dominicp9365]

RE sites can be 4, 6, 8 NT long.

[@lavanyatalagapu1509]

good explanation

[@Yitoamaya]

how could you use the same restriction enzyme to cut an espcific gene from some DNA and also the plasmid, if thoose enzymes are especific?

[@Yitoamaya]

or how is it done when introducing for example the insuline gene in E coli :P

[@MrTRANnysaurus]

Yitoamaya it is because they are specific to restriction sites not the sequence of the gene itself. These restriction sites flank the gene you are attempting to cleave. For example, EcoRI cuts at a 5'GAATTC3' palindromic sequence to produce a sticky end. There are many other restriction enzymes that cut in this manner and are specific to other restriction sites.

[@taghreadshaker9376]

Yitoamaya

[@asifansar8546]

I like it

[@swagemporer7171]

he sounds like he's in pain

[@audreypronovost6430]

Oufff by the way EcoR1 cut after the G... 5’G/AATTC 3´

[@maazarif6936]

bio Ncert based explanation......👍🏻👍🏻

[@meenakshinidasanametla3244]

interesting video

[@DragonLutte]

that Escherichia pronounceing.

[@aryanraj6906]

About mutation

[@kiran_kaur]

3:08 he missed one 'A'

[@mistrotman420]

I feel like people make educational videos terrible on purpose

[@vijayachandrakalyani779]

Just reading not explaining

[@amitdey4694]

nice

[@7activestudio]

Now You can Access 120+ hours of digital content for free in APP
play.google.com/store/search?q=sciencetuts

[@akashkumar9]

Ncert based and nice.

[@thekingmaker2160]

NCERT 😂

[@curvinmathebula8232]

I couldn't hear anything because of that very loud beautiful music in the background but then then the parts that I could hear were fine