Single molecule localization microscopy of extracellular vesicles

Lecture about single molecule localization microscopy (SMLM) of extracellular vesicles (EVs) by Tijana Jovanovic-Talisman as part of the third massive online open course (MOOC) of the International Society for Extracellular Vesicles (ISEV). The lecture covers the basic principles of single molecule localization microscopy, how to interpret the measured signals, practical information, do’s and don’ts as well as examples.