Determination of Crude Fiber Content -A Complete Procedure (AOAC 978.10)

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  • Опубликовано: 4 дек 2024

Комментарии • 377

  • @senalpha
    @senalpha 2 года назад +11

    I am a teacher of Laboratory Chemistry in an italian upper school of sanitary biotechnology. This is the Weende Method for the determination of crude fiber in foodstuff for human consumption and animal feed. I will do it with a 4th year classroom in a couple of weeks using your method. Thank you for your very nice and uselful video. Chemistry made simple. I often follow lessons of US universities on line. Thank you again guys, long live US and long live Italy !!!!!!!!!!!

    • @MicroChemsExperiments
      @MicroChemsExperiments  2 года назад +1

      Thank you so much for your compliment. I hope that you will share this platform in your circle. Stay with us.

    • @VishnuVishnu-bj1iw
      @VishnuVishnu-bj1iw 7 месяцев назад

      Hii i.m.from India,I m working as quality control chemist of rice bran oil company any vacancies,and I m Post graduate in MSc organic chemistry (2021) passed out

  • @saadbinmasud
    @saadbinmasud Год назад +4

    as a student of Veterinary Medicine this lesson helps me in Animal Nutrition course final exam..
    thanks 😊

  • @hafizullah9137
    @hafizullah9137 3 года назад +6

    A series of such videos are needed for every type of proximate analysis like protein, fat.....
    Thank You for the good work. Keep the Good work.

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад +2

      Thank you. Stay with us

    • @foodfeedinfo
      @foodfeedinfo 2 года назад

      #NDF_Determination.
      ruclips.net/video/ip2M_4xvdhQ/видео.html
      The more readily utilizable nutrients are enclosed by the cell wall and can be grouped as cell contents. The cell solubles are extracted by boiling with neutral detergent solution containing sodium lauryl sulfate and then cell wall constituents (NDF) are separated by filtration.

  • @jagadishnc7923
    @jagadishnc7923 Год назад +3

    Since I am in process development this video is very useful to me for Analysis, thank you❤🌹🙏

  • @Asmasiddique-ke8ju
    @Asmasiddique-ke8ju 6 месяцев назад +1

    So Organized and Understandable Video ❤

  • @sirimath111
    @sirimath111 3 года назад +1

    Excellent, orderly demonstration 👌

  • @surendranathsingh8040
    @surendranathsingh8040 Месяц назад

    Very good teaching. Sir
    Thanks

  • @dr.ajaybarman7041
    @dr.ajaybarman7041 3 года назад +1

    Very helpful video...keep making this kind of videos.. I hope ,oneday your video will be recognised well..

  • @Chandra6E5176
    @Chandra6E5176 2 года назад +2

    Thank you , I'm directly in last semester of my Master's after corona lockdown and needed this so much 💯 Thank you so much Guru

  • @jancy98
    @jancy98 Год назад +1

    Should we use the defatted sample for the determination of crude fibre?

  • @SiddiqiRaashid
    @SiddiqiRaashid 2 года назад +2

    I believe the sample should be placed in a round bottom flask or in your case conical flask with a condenser so that the acid or base should not evaporate. Plz look into it .

    • @MicroChemsExperiments
      @MicroChemsExperiments  Год назад +1

      You are correct. The way you are saying will be the most accurate method.

  • @monicanyamu2207
    @monicanyamu2207 3 месяца назад

    Thank you very much.

  • @ogubenson5098
    @ogubenson5098 3 года назад +2

    I kept hearing filtrate while you transferred the residue to the second conical flask to boil in the base. Please what exactly is it? Great video though, I must say. Thanks so much

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      Filtrate is what that is left on the filter paper after the filtration process.

    • @drupenymphaelilly1660
      @drupenymphaelilly1660 3 года назад

      @@MicroChemsExperiments no its the residues as the filtrate passed from the filter paper into the conical flask

    • @drupenymphaelilly1660
      @drupenymphaelilly1660 3 года назад

      You are right dear......no its the residues as the filtrate passed from the filter paper into the conical flask

  • @rajeshsiva2257
    @rajeshsiva2257 7 месяцев назад +1

    Hi. Is fitting of condenser is necessary in the standard procedure ?

  • @oussamasemaili9630
    @oussamasemaili9630 2 года назад

    Je vous remercie infiniment c'est tellement clair

  • @guruprasadk3848
    @guruprasadk3848 3 года назад +2

    Hai... I guess we need to defat the sample i.e. remove the fat content by soxhlet extraction using Petroleum ether before proceeding for crude fiber estimation... Please share your thoughts on this

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад +2

      In AOAC method, it is not said to defate the sample before coducting the Crude Fiber Test. But if you want, you can defat your sample and there will be no problem with thr Fiber Test result.

    • @darshanirg215
      @darshanirg215 3 года назад +1

      if we dont do the defating for spices sample then, when calculating the residue become high and the results goes high. But when do the defat then get the normal results. So is it must to do the defat for spices (>10 fat sample)

  • @rajesh7999
    @rajesh7999 4 года назад +4

    You can use silicon anti foaming agent to stop the froath and also can connect water condenser to the flask for good digestions

    • @MicroChemsExperiments
      @MicroChemsExperiments  4 года назад +1

      NUTHAKKI RAJESH, Thank you for your comment. We should use anti-foaming agent and water condenser. Obviously it is a good practice. Thank you for your observations.

  • @ashalathaaribail3112
    @ashalathaaribail3112 3 года назад

    Thank you so much for this video

  • @raihaha
    @raihaha 2 года назад

    Thank you thank you thank you!!!!

  • @khadeejanasrint8031
    @khadeejanasrint8031 Год назад +1

    Sir we did this experiment for our ug project by using the animal feed. but, How will we confirm that the extracted substance is a crude fiber??an animal feed containing many substituents other than fiber.??and how will we establish that the ash content is minerals ?

  • @MuhammadFarhan-gm1sc
    @MuhammadFarhan-gm1sc 3 года назад +1

    Thank you for the great video. May I know why we need to incinerate the fiber in this analysis. Thanks in advance.

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад +1

      Ash (Minerals) is not a part of fiber. So you must subtract the minerals or ash content.

    • @Kfri4486
      @Kfri4486 3 года назад

      @@MicroChemsExperiments Hello! Thanks for the video. Can we just then get the value for ash in the sample also by doing this fiber procedure?

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      @@Kfri4486 no. You can't.
      You have to follow different method for ash determination. Link: ruclips.net/video/n2Qwb8Pw8YE/видео.html

    • @Kfri4486
      @Kfri4486 3 года назад

      @@MicroChemsExperiments thank you for the prompt answer

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад +1

      @@Kfri4486 You are most welcome

  • @mdsoyel6847
    @mdsoyel6847 3 года назад +1

    Nice explanation...but i have a question what are the needs of heating the sample with acid and a base?

    • @MicroChemsExperiments
      @MicroChemsExperiments  2 года назад +3

      To remove acid and base soluble materials from the sample

    • @olaideakinwumiakintayo7295
      @olaideakinwumiakintayo7295 2 года назад

      @@MicroChemsExperiments Thanks for your response. But I suppose the question is not why we use the hot water, which is what I think you answered, but why we use acid and base.
      I think that is to remove organic materials from the sample such as carbohydrate and protein

  • @asmahmud2315
    @asmahmud2315 4 года назад +1

    It's very helpful keep it up.

  • @shankarsahu8340
    @shankarsahu8340 2 месяца назад

    Thinks sir ❤

  • @sarahkandolo6735
    @sarahkandolo6735 4 года назад +2

    Hey. How did you guys do the calculations? What volume is needed for sulphuric acid and what grams are needed for the pellets. Please help..

    • @MicroChemsExperiments
      @MicroChemsExperiments  4 года назад +2

      Thank you Sarah Kandolo for your queries.
      I used 3.5 ml sulfuric acid-98% to make final 500 ml of 0.128M sufuric acid solution and I used 6.25g of NaOH pellets to make final 500ml of 0.313M NaOH solution, as described by the AOAC 978.10 method. I followed the AOAC 978.10 method strictly throughout the test.

    • @foodfeedinfo
      @foodfeedinfo 2 года назад

      #NDF_Determination.
      ruclips.net/video/ip2M_4xvdhQ/видео.html
      The more readily utilizable nutrients are enclosed by the cell wall and can be grouped as cell contents. The cell solubles are extracted by boiling with neutral detergent solution containing sodium lauryl sulfate and then cell wall constituents (NDF) are separated by filtration.

  • @zijietan8953
    @zijietan8953 3 года назад +1

    @MicroChem's Experiments May I know if tempe is used to determine the crude fibre , do we need to defatted the tempe sample first by using soxhlet method ?

  • @kavyaraktajihva3733
    @kavyaraktajihva3733 3 года назад

    Thank you brother 🙏🇮🇳

  • @maahii8335
    @maahii8335 3 года назад

    Thank u soo much sir excellent explanation 😊😊

  • @ParveenSayyad-b1f
    @ParveenSayyad-b1f Год назад

    Excellent

  • @asadurrahman319
    @asadurrahman319 2 года назад

    great content thanks a lot sir

  • @aparnasahu6448
    @aparnasahu6448 3 года назад +1

    Nice vedio, but i have a doubt on calculation part, as it said to deduct the weight of crucible with from that of fibre, but while in doing show it was shown to substracting wt of crucible with ash from that of with fibre. So what I want to know is if the formula is wrong or the values are put wrongly.

    • @MicroChemsExperiments
      @MicroChemsExperiments  2 года назад +1

      Formula is correct.
      Basically ash content is not the part of the fiber. Thats why ash wt is subtracted from the fiber wt.

  • @shinylife150
    @shinylife150 Год назад +1

    Hot water used to wash residue is distilled water or tap water?

    • @noodles8439
      @noodles8439 9 месяцев назад +1

      I think all the water used to deal with the sample should be distilled or purer water.

  • @tahabazine3397
    @tahabazine3397 4 года назад +1

    We put the crucible inside the Muppled Furnace for 2 hours or 4 hours ?

    • @MicroChemsExperiments
      @MicroChemsExperiments  4 года назад +2

      Taha Bazine, 2 hours is enough to make ash of the fiber content. So we kept the crucible in Muppled Furnace for 2 hours

    • @padmaladon8739
      @padmaladon8739 3 года назад +1

      It showed 4 hours too... may be it was including the cooling time till it reached 250 degrees C.

  • @yuvarajghosh2789
    @yuvarajghosh2789 Месяц назад

    Is this method applicable for germinated millets

  • @geberemaryamterefe7721
    @geberemaryamterefe7721 3 года назад

    Thank you

  • @ashrafulbizoy8048
    @ashrafulbizoy8048 6 месяцев назад

    temperature of hot plate? do i need magnet? can i determine orange peel's fibre?

  • @e-man3584
    @e-man3584 3 года назад +1

    How do you account for sample loss when taking sample from the linen to crucible?

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад +1

      Sample will not be lost if you take care during transfering. Or may be the loss of sample is not significant.

  • @taharhanafi7928
    @taharhanafi7928 2 года назад

    Thanks

  • @ramshatahir8860
    @ramshatahir8860 3 года назад

    Nice demonstration. Kindly give me the reference from which research paper you've taken this experiment.

  • @sakirhossain2016
    @sakirhossain2016 3 года назад +1

    Is it Molar soln or Normal soln?Cause For . 128 Molar Soln it may be needed 6.272 ml H2So4 in 500 ml water?!

    • @sakirhossain2016
      @sakirhossain2016 3 года назад

      Eagerly waiting for your response Dear...

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад +1

      Thank you for your patience. It should be Molar which is already given in this video. According to AOAC official method, the H2SO4 solution prepared in this video is Molar solution. If you have any confusion with it, please read the AOAC reference method mentioned in this video title.
      Thank you.

    • @sakirhossain2016
      @sakirhossain2016 3 года назад

      Thank you for your response. Request to recheck and I will try also.

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      @@sakirhossain2016 We rechecked your confusions from AOAC method.

  • @biancaysabelvicente215
    @biancaysabelvicente215 3 года назад +1

    Hi Sir, why should the boiling be continued for exactly 30 minutes? Please help

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад +2

      Because boiling of sample for 30 minutes is needed to dissolve extra materials other than fiber, in acid and base and finally remove by filtration.

    • @biancaysabelvicente215
      @biancaysabelvicente215 3 года назад

      @@MicroChemsExperiments Thank you so much, for responsing and for doing this kind of videos, your videos are very helpful

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад +1

      @@biancaysabelvicente215 Thank you too for being with us.

  • @user-rv3nz7fd6j
    @user-rv3nz7fd6j Год назад

    Thank you very much, sir.
    Can you send me the source used for this method or the link to obtain the chemical analysis of foodstuffs because I find it difficult to obtain the source used

    • @user-rv3nz7fd6j
      @user-rv3nz7fd6j Год назад

      @MicroChemsExperiments

    • @user-rv3nz7fd6j
      @user-rv3nz7fd6j Год назад

      Is the same method used to estimate dietary fiber in rice, and can you provide me with AOAC or AACC methods with the latest copies?

    • @user-rv3nz7fd6j
      @user-rv3nz7fd6j Год назад

      @MicroChemsExperiments

  • @Limelight530
    @Limelight530 4 года назад +1

    I have to extract fiber from potato peel kindly suggest me any procedure .

    • @MicroChemsExperiments
      @MicroChemsExperiments  4 года назад +1

      Zainab Shoukat, you can follow the method, described in this video, without any doubt. But before going for the test, you have to blend and grind the potato peel sample properly.

    • @Limelight530
      @Limelight530 4 года назад

      @@MicroChemsExperiments thank you so much

    • @MicroChemsExperiments
      @MicroChemsExperiments  4 года назад

      @@Limelight530 welcome

  • @irhaachaudary1638
    @irhaachaudary1638 11 месяцев назад

    How can we determine crude fiber? Which method use?

  • @imim1729
    @imim1729 2 года назад

    Thank you for the methode it's very helpful, just a question how can i reach the paper of the methode??

    • @MicroChemsExperiments
      @MicroChemsExperiments  2 года назад +1

      No. You can't. You can just take note from this video

    • @imim1729
      @imim1729 2 года назад

      @@MicroChemsExperiments but i need it for my research please 🙏

    • @MicroChemsExperiments
      @MicroChemsExperiments  2 года назад

      @@imim1729 from which country you are?

    • @imim1729
      @imim1729 2 года назад

      @@MicroChemsExperiments Algeria

    • @MicroChemsExperiments
      @MicroChemsExperiments  2 года назад +1

      @@imim1729 knock on our Facebook page.

  • @darshanirg215
    @darshanirg215 3 года назад

    can we use octanol as a anti bumping agent for base digeation .?

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      It is not suggested to use. Use glass bead to avoid any kind of error

  • @hebasayed-d5h
    @hebasayed-d5h Год назад

    please can i calculate : the weight of crucible with fiber - the weight of crucible only , instead with ash , yes or no and why ?

  • @raniyafasil7790
    @raniyafasil7790 3 месяца назад

    In our lab, we use gooch crucible for crude fibre determination.. then what is the need of this gooch crucible instead of this type of crucible??

  • @Kfri4486
    @Kfri4486 3 года назад

    Hello! Thank you so much for the video. I have one question. I performed this experiment and after the NaOH digestion, I ended up without any sample, everything filtered through the cloth. Do you have any advice on this matter? Thank you!

  • @romeites6398
    @romeites6398 3 года назад

    Simple question. What kind of food makes stool soften?

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      Low fiber containing food

    • @romeites6398
      @romeites6398 3 года назад

      @@MicroChemsExperiments For example of foods?

    • @romeites6398
      @romeites6398 3 года назад

      @@MicroChemsExperiments I hope you reply it soon. I need examples of food

  • @zhijunlim5769
    @zhijunlim5769 3 года назад

    Hi there, I have some question need for yours response. 1. The sample size is there any recommendation (base on the % of the crude fiber)? 2. Does this 2g of sample is fixed? Does the method have the maximum sample weight for the acid and alkali which is enough to digest? 3. If I increase the sample size, do I need to increase the boiling time of both acid and base? 4. 2g vs 2.5g of same sample will be have any difference in the crude fiber obtained?

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      In AOAC official method, sample size is declared as around 2g. But I think 3g sample can be used but not sure about the sample size of more than 3g.
      To follow AOAC method strictly, we should always use 2g sample. Sample size should not be increased considering the expected fiber content.

  • @priyanshimartin8582
    @priyanshimartin8582 3 года назад

    Hey ! I forgot to do the step when you put the crucible with sample in hot air oven but I placed the crucible in muffle furnace at 130 degree celsius for 2 hours .. So what should I do ? Should I repeat the procedure or should I put the sample again in muffle furnace but this time at 550 degree for 2 hours ? Please help !! 🥲

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад +1

      Normally in muppled furnace, low temperature (100-250 degree Celsius) can not be applied correctly, because it is designed for high temperature applications.
      Many times we saw that, when we set the furnace temperature at 130 degree Celsius, the actual temperature rises above 150 degree Celsius.
      But if you ensure that the actual furnace temperature was stable at 130 degree Celsius for two hours then there is no need to repeat the test. Just take weight and make ash using furnace again doing the final Fiber calculation.

  • @win15_13
    @win15_13 3 года назад

    In determining the crude fiber content of what sample do you use?

  • @mathiasbee3155
    @mathiasbee3155 3 года назад

    Hi can I skip the oven drying method and go straight to the furnace step instead?

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      No. Oven drying and after that taking weight is mandatory.

    • @mathiasbee3155
      @mathiasbee3155 3 года назад

      Thank you for the reply and another last questions isit okay if I stop at the step where we collect the residue and leave it in the crucible, then we will run them in the oven and furnace after two days?

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      @@mathiasbee3155 Yes. It is okay

    • @mathiasbee3155
      @mathiasbee3155 3 года назад

      Thank you for the reply...appreciate it 👍🏻

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      @@mathiasbee3155 Stay with us

  • @abinayas3752
    @abinayas3752 2 года назад

    Good evening sir. I have one doubt. The wash water should be distilled or can I use tap water also

  • @UnknownHakim
    @UnknownHakim 3 года назад

    May i know. What kind of this method is? For example, for antioxidant we can use DPPH method.

  • @AmenGetaye-k7h
    @AmenGetaye-k7h 9 месяцев назад

    Please provide coffee husk fiber extraction by alkaline treatment

  • @qiaowei230
    @qiaowei230 4 года назад

    Hi, can I dry the samples that after treated with acid and alkaline by using hot air oven at 105 degree overnight instead of 230 degree for 2 hours?

    • @MicroChemsExperiments
      @MicroChemsExperiments  4 года назад +1

      Qiaowei230, This drying is needed only for the removal of moisture content from the fiber content. Drying at 130 degree Celsius for 2hours is enough for the purpose according to the AOAC method of Moisture determination. Long time drying at 105 degree Celsius could be a reason of loss of some fiber content. So you should follow the standard method to analyse any sample to get the accurate results.

  • @khadeejanasrint8031
    @khadeejanasrint8031 Год назад

    Sir ,Which sample is taken for the extraction of crude fibre.will you please suggest some available fibre content sample?

  • @luisarmas2366
    @luisarmas2366 3 года назад

    If I use the fiber analysis by the AOAC 962.09 method, is it also correct? Fiber incineration is not considered in this analysis.

  • @piyushprajapati6080
    @piyushprajapati6080 4 года назад +2

    Can we use magnetic stirrer to stirr the solution while heating (boiling) with acid and base? you had to stirr conical flask with hands.

    • @MicroChemsExperiments
      @MicroChemsExperiments  4 года назад +3

      Piush Prajapati, If you use magnetic stirrer continuously during boiling, then foam will be form and which will overflow the flask and can cause loss of the sample.
      If you want to use magnetic stirrer then you must have to use anti-foaming agent with the sample during boiling to prevent foam formation.

  • @coffeegirl7426
    @coffeegirl7426 6 месяцев назад

    Thank you so much this is really helpfull..but can you please confirme the temperature of the hot air oven..u said 130° and you wrote 230°C...
    And please confirme the time of incerination u said 2h then u desiccated after 4h...
    I think it's 4h coz the T is 550°C .
    Thank you

    • @MicroChemsExperiments
      @MicroChemsExperiments  6 месяцев назад

      You are correct. Temperature is 130 degree Celsius and incineration for 4 hours

    • @coffeegirl7426
      @coffeegirl7426 6 месяцев назад

      Thank you so much sir

  • @onitnelovabequibel8759
    @onitnelovabequibel8759 2 года назад

    Hello I have a question Why is the paper stained with the different test solutions in the determination of fiber composition?

  • @surjitdeka7092
    @surjitdeka7092 2 года назад

    Sir I have developed a syrup by combining the varius fruit extract so that vitamin c and e can be added .
    Now can i determine the fibre content in tha syrup ? generally fibre is not found in syrup m I right?
    So what should I do ... determination of crude fibre or dietary fibre

  • @parthsakhavala1764
    @parthsakhavala1764 Год назад

    Sir plant sample is taken and required a defat with ether or not

  • @bijoymaitra
    @bijoymaitra 3 года назад

    Is the method suitable for plant leaves?

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      Yes. This method is also applicable for plant leaves and vegetables

    • @bijoymaitra
      @bijoymaitra 3 года назад

      @@MicroChemsExperiments For plant leaves,, should we take the weight of raw leaves directly or should we take the weight after drying in oven??? What's your idea about it.

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      @@bijoymaitra You can used raw leaves to test if you consider the raw leaves as sample. But if you wish to test the sample on dry basis then you have to dry it in over before testing.

  • @sumeet1769
    @sumeet1769 Год назад

    can i use this method for estimate crude fiber in millet rusk/toast ?

  • @noodles8439
    @noodles8439 9 месяцев назад

    I read some standards and methods for fiber determination, and I have some experience in the lab. However, I still think there are a lot of improper operations in the video, like making the solution directly in the volumetric flask even if the regent generates heat when it is dissolved in water. and for the collection of the residue, I deeply doubt that the scratch movement can collect all fiber and had no influence on the real result. besides, the white cloth can be used as the filter paper? it's too casual in my opinion.

    • @MicroChemsExperiments
      @MicroChemsExperiments  9 месяцев назад

      Thanks for you comment. Actually this is only for beginner to get their concepts clear in a short video

    • @noodles8439
      @noodles8439 9 месяцев назад

      anyway, all the lab work should be done in a very cautious safety and serious way. even if it is only for showing the concepts.@@MicroChemsExperiments

  • @heart_ofkkr.i8953
    @heart_ofkkr.i8953 Год назад

    Please share calcium phosphorus test procedure

  • @ovaneschakoyan6584
    @ovaneschakoyan6584 3 года назад

    What type of filter paper with vacuum filtration device can i use please or exists clogging problem

  • @lakhanyadav6205
    @lakhanyadav6205 3 года назад

    Sir please make video for urea activity check method in food all 3 method.

  • @khadijatogunyemi6756
    @khadijatogunyemi6756 2 года назад

    Good day sir, can you please tell me the name of the sample you used?
    Thank you

  • @log.mochi128
    @log.mochi128 3 года назад

    i would like to ask why we need to remove the moisture content from the sample?

  • @yousraboukssessa4849
    @yousraboukssessa4849 2 года назад

    this method is valid for plants please

  • @nabilaislam531
    @nabilaislam531 3 года назад

    Thank you so much for this video😊😊😊 I have a question if i want to determine the crude fiber in fruits or vagetables then how can i do the sampling??

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад +1

      Collect your sample and bend the fruits & vegetable to make it homogeneous which contains each portion of the sample in same proportion. Then from the homogeneous mixture of the sample, take 2g for testing.

    • @nabilaislam531
      @nabilaislam531 3 года назад

      @@MicroChemsExperiments Thanks a lots

    • @nabilaislam531
      @nabilaislam531 3 года назад

      @@MicroChemsExperiments will you tell that before determining crude fiber do i have to make the sample fat and moisture free

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      @@nabilaislam531 Most welcome

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад +1

      @@nabilaislam531 no. You dont need to remove moisture and fat from the sample. Test the raw sample as you received or collected.

  • @nursuhaizamohdfauzi4409
    @nursuhaizamohdfauzi4409 3 года назад

    Can we used filter paper instead of cloth?

  • @ravi.jakhal
    @ravi.jakhal 3 года назад

    What is the time of incineration?
    2 hour or 4 hour?

  • @PriyaKumari-ti6mi
    @PriyaKumari-ti6mi 2 года назад

    What does this fibre content indicate about adulteration of the cereal(sample) ?

  • @ireshanishadi9865
    @ireshanishadi9865 2 года назад

    Is this method good for rice

  • @thirulogasundharbalakamatc5254
    @thirulogasundharbalakamatc5254 2 года назад

    Can you please specify the mesh size for the cloth?

  • @itsmydayandurday
    @itsmydayandurday 2 года назад

    Hi! I want to determine the dietary fiber of jam. Can I use the sample in its wet form and without defatting it? Hopefully in the future you can make videos about determining dietary fiber😁

    • @MicroChemsExperiments
      @MicroChemsExperiments  2 года назад

      Yes. you can

    • @itsmydayandurday
      @itsmydayandurday 2 года назад

      @@MicroChemsExperiments Jam contains very high sugar, for that is it necessary to desugar it first?

    • @MicroChemsExperiments
      @MicroChemsExperiments  2 года назад

      @@itsmydayandurday Method says that desugar is not necessary because high sugar is minimised taking low volume of prepared sample in the titration flask.

  • @abebawewunetu8829
    @abebawewunetu8829 3 года назад

    please show protein analysis

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      We have already made video for protein determination. Please search it in this channel

  • @nadianadzirah8910
    @nadianadzirah8910 3 года назад

    is it can be used for liquid sample like fruit juice?

  • @Limelight530
    @Limelight530 4 года назад

    This can be also known as the extraction of fiber from any sample?

  • @narendarpratapyadav9908
    @narendarpratapyadav9908 2 года назад

    Hello
    Sir this process use in every product of fiber let maize gluten ,dry maize ,csl mix fiber... Please give me suggestions ,

  • @palas987654321
    @palas987654321 3 года назад

    Hi, Can I able to implement this method for coconut fiber content determination?

  • @nursyazwani6680
    @nursyazwani6680 2 года назад

    what the temperature used during boiling point in acid ( h2so4 ) ?

    • @MicroChemsExperiments
      @MicroChemsExperiments  2 года назад

      140 degree Celsius

    • @nursyazwani6680
      @nursyazwani6680 2 года назад

      @@MicroChemsExperiments okey thankyou ! your vid very help to success our project 😊🙌🏼

  • @Limelight530
    @Limelight530 4 года назад

    For drying temperature is 230* or 130* ? U wrote 230 while in the sound it is 130 degree

    • @MicroChemsExperiments
      @MicroChemsExperiments  4 года назад +1

      Zainab Shoukat, Thank you for your observations. Drying temperature should be 130 degree Celsius. 230 is our typing mistake and we are very sorry for that.

    • @Limelight530
      @Limelight530 4 года назад

      @@MicroChemsExperiments OK Np

  • @zijietan8953
    @zijietan8953 3 года назад

    Hi. Which year of AOAC 978.10 used in this experiment?

  • @aksiddiki0
    @aksiddiki0 4 года назад

    Can i use whatman filter paper instead of cotton cloth?

    • @MicroChemsExperiments
      @MicroChemsExperiments  4 года назад +2

      Ak siddiki, no, you can't filter using whatman filter paper because of it's tiny pore size. After boiling the feed sample in NaOH solution, relatively more dense & thicker solution is produced which is not possible to filter using Whatman filter paper. Cotton cloth is most desirable for this purpose.

    • @informativemixvid9395
      @informativemixvid9395 3 года назад +2

      You can use whatman, im using it when I was doing proximate analysis, too slow by gravity, but you can use vacuum pump it to suck up the liquid leaving solid particles of sample.

    • @aksiddiki0
      @aksiddiki0 3 года назад

      🤔

  • @ramlak5034
    @ramlak5034 Год назад

    Do more video in species

  • @romeites6398
    @romeites6398 3 года назад

    Can you make experiment with real fruit or soluble fiber?

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      You can do fiber test of real fruits by this method described in this video.

  • @jayeshlohar1269
    @jayeshlohar1269 3 года назад

    the fiber content we got was total fiber content, I mean soluble + Insoluble fiber?

  • @कुंवरभवनीकसरवालराणा

    Please mention Boiling temperature !?

  • @milkimojo6849
    @milkimojo6849 3 года назад

    what is the problem if we not cover crucible with it lid

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад +1

      Loss of ash may occure during burning of sample at high temperature for Ash dwtermination method. But in crude fiber test, loss of ash will not happened as the content in crucible is very small amount. So you can keep the crucible open in this test.

  • @subhojitbhowmick3966
    @subhojitbhowmick3966 Год назад

    Sir please ...I need a video for collour of oil .....

  • @nainarazzaqnizami5694
    @nainarazzaqnizami5694 10 месяцев назад

    hello ,
    AOAC 978.10 is for fiber determination in feed stuff not for food sample

  • @affanghani9149
    @affanghani9149 3 года назад

    What temperature is require in first and 2nd time geating solution

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад +1

      Affan Ghani, there is no specific temperature. Set the temperature at Boiling point (above 110 degree Celsius)

    • @affanghani9149
      @affanghani9149 3 года назад

      @@MicroChemsExperiments thanx

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      @@affanghani9149 You are most welcome.

  • @pavithradesu
    @pavithradesu 3 года назад

    How to find the fibre content in glass & kevlar fiber composite materials?

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      This method is selectively applicable for Food, Feed, Fruits, Vegetables, Environmental sample and related sample.

    • @pavithradesu
      @pavithradesu 3 года назад

      @@MicroChemsExperiments Okay sir...Thank you

    • @MicroChemsExperiments
      @MicroChemsExperiments  3 года назад

      @@pavithradesu You are welcome.

  • @thalassophile23
    @thalassophile23 2 года назад

    Excuse me, I want permission to ask. Where can I see the full procedure for the 2005 AOAC crude fiber test? Where can I access the document/pdf? thanks :))