9. Isoenzymes or Isozymes

Definition
different forms of the same enzyme catalyzing same reaction
How they are formed
1. Different genes- true isozymes
2. all variants are present in same individual
3. isoforms- due to post translational modification
4. allozymes- from different alleles
characteristics
1. electrophoretic mobility
2. substrate specificity and Km
3. Heat stability
4. Inhibitor
5. Coenzymes
6. Antibody sensitivity
7. Tissue of origin
examples
LDH- 1 to 5
CK 1 to 3
ALP-6
clinical significance

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Thanking

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sir the LDH start peak and normal value is different from what u told in viedo of cardiac markers

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I didn't understand heat labile property of isoenzyme ??

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Please suggest any good book for enzymology in english.
Thanks

[@Rajesh_Jambhulkar]

Vasudevan for MBBS

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Thank you very much.
Im a biotech student looking for Ph.D, I'll surely search for the book you suggested.

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[@pmuralidharan5042]

Sir you have said that alpha 2 HL is present in liver and is sensitive to heat but in my textbook it is given as another isoenzyme of ALP that is present in bone is also heat labile.
Can two of isoenzymes be heat labile sir ?

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Sherrrrrrrrr 🐅

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Sir. Normal range OF ALP is 30 to 300 or 30 to 120 IU/L???

[@aasemahsan]

11:32 LDH
13:59 CK (Creatine Kinase)
15:57 ALP (Alkaline Phosphatase)

[@dr.khairunnissa9822]

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CK-M peak is also differnt

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Bhai isoenzyme satyanarayan me kis page pe hai?

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What is the 6th isoenzyme of alkaline phosphatase ??

[@Rajesh_Jambhulkar]

Leukocyte alkaline phosphatase

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Sir H3m and H2m2 ..where they present

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[@Rajesh_Jambhulkar]

Yes