Warning to watchers, he explains the fragment collision with the detector wrong as he does in the previous part too (the correct collision is: lowest mass will hit the detector first because they are being deflected more by the magnetic field than the higher mass fragments). So the correct order of hitting the detector would be m3, m2, m1. Not what he proposes around 20 minutes in.
The lines of magnetic field should be orthogonal to your whiteboard to have an ions deflection as you sketched. Moreover, increasing of the magnetic field should be represented by increasing the lines density and not by decreasing them.
There is no need of this video shomu dada.. Your videos are good and very helpful but in this 35 minute video most things are very repetitive.. Best of luck dada you are doing a good job.. 😊
Hi Suman! Thanks for your video, but i have a question: why in the amplification part you're talking about electrons, when we have the positively charged particles? Are those electrons belonging to them?
Shomu Bhai.. u mentioned in the detector that electrons hit the dinodes.. but as u had said earlier.. the electrons are already taken out from the ionizing chamber right ? now the ones which are left are molecular fragment ions which travel through the field and hit the detector.. then how come electrons came into picture to hit the detector..?
molecular ions have positive charge but they have an unpaired electron also,he represented molecular ion as Mpositive with a dot (M+.)..which means it has an unpaired electron.
Here, in previous vids of shomu sir, he has explained it in detector part. in detector, there are dynodes present which are series of electrodes of varying voltage. when molecular ions or fragment ions hit the detector, they cause release of secondary electron from the surface of the electrode due to hitting/collision. so these electrons are then amplified through these electrodes to get strong signal and then are collected into the wire which generates flow of electrons i.e current. this current is then recorded in computer and converted into graphical representation. Meanwhile, when molecular ion hits the electrode, it releases sec. electrons from surface of electrode, so one of the electron is gained by molecular ion and gets neutralised on the 1st electrode. (note: number of sec. electrons produced depends upon the characteristics of the molecular/ fragment ion) hope this will help you. god bless.
Sir you tell the electron attacked to the dynodes of detector but What is the source of electron...? (what is the procedure to remove electron from fragment ion?)
Sir please tell why you talked about electrons and nit the positively charged fragment ions? In the detector.. Because the positive ions fragments are hitting the detector but then suddenly you takked aboutvelectric signal amplification
Warning to watchers, he explains the fragment collision with the detector wrong as he does in the previous part too (the correct collision is: lowest mass will hit the detector first because they are being deflected more by the magnetic field than the higher mass fragments). So the correct order of hitting the detector would be m3, m2, m1. Not what he proposes around 20 minutes in.
@shoumosbiology is it the correct order?
Wow❤️❤️❤️
Lecturers of my college should learn from you how to explain a term in a perfect way🙏🙏
Lot's of love and blessings..💐💓❤️
Thank you very much i have a exam in 10 days and i have no idea what to do!
Finaly tk to you i understand MS!!
thank you very much. I knew nothing about this topic before, now I'm feeling like I'm going to specialise in it, and it's for free.. at home. thanks.
+Sham Thorani I am glad that you are getting benefit from the videos
Really appreciable and helpful in compared to our college lecturers
Thank you
The lines of magnetic field should be orthogonal to your whiteboard to have an ions deflection as you sketched. Moreover, increasing of the magnetic field should be represented by increasing the lines density and not by decreasing them.
this is great job, I like your explanation, just some time you make me confuse
I am really appreciated for the video as it was really helpful for me.
+habib boroun thank you. Glad you liked my lectures
Legend on the scene
This video is really helpful for me 😊👍
You're welcome
u are great teacher sir
There is no need of this video shomu dada.. Your videos are good and very helpful but in this 35 minute video most things are very repetitive.. Best of luck dada you are doing a good job.. 😊
this is so good explanation......god bless you sir
Thank you for watching the lecture. Please subscribe and share
Very nice bro ....u r a great teacher
Best way of learning thank you sir
Very helpful sir!
You're welcome
Thank you for easy to make it understand.
You're welcome. Glad to hear that you're getting benefit from my lectures
I like all your videos
Good initiative.
Thank you
how r u explaining this better than my lecturer who i pay 9k
Thank you. Glad you liked my lectures
Super sir pls continue this work
Thank you
thanks for the vid shomu..
Thnkkks, You re amazing.
You're welcome. Glad to hear that you're getting benefit from my lectures
@@shomusbiologyofficial i should be thanking u sir as i'm indeed benefiting from u. May God increase u in knwledge🙏
Hi Suman! Thanks for your video, but i have a question: why in the amplification part you're talking about electrons, when we have the positively charged particles? Are those electrons belonging to them?
was thinking this
sir plz video on flame photo metry and atomic spectroscopy
Please make a vidio on phosphoprotiomics plz.
Tq u sir for your lecture
The magnetic field direction should be pointing perpendicular into your whiteboard according to the right-hand law.
Thnku sir from Manipur
You're welcome
very good teachs sir
Nice sir Ji
Thank you Sir...
Please do a vidio regarding tandam mass spectrometry
well explained bro
Very helpfull
Shomu Bhai.. u mentioned in the detector that electrons hit the dinodes.. but as u had said earlier.. the electrons are already taken out from the ionizing chamber right ? now the ones which are left are molecular fragment ions which travel through the field and hit the detector.. then how come electrons came into picture to hit the detector..?
molecular ions have positive charge but they have an unpaired electron also,he represented molecular ion as Mpositive with a dot (M+.)..which means it has an unpaired electron.
Krishna Kulkarni i think after fragmentation the electrons are generated are being detected
Here, in previous vids of shomu sir, he has explained it in detector part. in detector, there are dynodes present which are series of electrodes of varying voltage. when molecular ions or fragment ions hit the detector, they cause release of secondary electron from the surface of the electrode due to hitting/collision. so these electrons are then amplified through these electrodes to get strong signal and then are collected into the wire which generates flow of electrons i.e current. this current is then recorded in computer and converted into graphical representation. Meanwhile, when molecular ion hits the electrode, it releases sec. electrons from surface of electrode, so one of the electron is gained by molecular ion and gets neutralised on the 1st electrode.
(note: number of sec. electrons produced depends upon the characteristics of the molecular/ fragment ion)
hope this will help you.
god bless.
Krishna Kulkarni that’s ryt it’s not electrons that hit but parts of the molecular ions nice observation
i also have a same question even it creats a confusion
Sir you tell the electron attacked to the dynodes of detector but
What is the source of electron...? (what is the procedure to remove electron from fragment ion?)
Sir please tell why you talked about electrons and nit the positively charged fragment ions? In the detector.. Because the positive ions fragments are hitting the detector but then suddenly you takked aboutvelectric signal amplification
You did not mention anything related to protein analysis.your discussion not according to topic's tittle.
why charged molecules can not be deflected under magnetic field ?
salem allikm
A lot of thanks sir, i have question about the detector , it detect fragment ions or electrons and if electrons;they produce from what .
can u please tell me reference books for mass???
hello sir can u produce any video for protein profiling process
hi do have a video on SILAC proteomics?
How is the single + and electron in molecular ion is distributed among fragment ion??
why not molecular ion be fragmented when passing through the electrode
Why is the magnetic field point upwards, whereas the fragmented molecules are being deflected downwards?
pointing*
Plz include the theory notes too
Sir can you please tell me the reference book for proteomics nd mass spectrometry for protein identification?
shouldn't deflection be in the direction of the magnetic field?
Suman sir u repeat things toooo muuchhhh,, plz go forward in appropriate time ,, kep less repetation ,,it. Too much boring
+Gs Virk Thank you for the suggestion
Could you share protein detection notes
But when are you talking about protein in this video? I see only a general explanantion, non specifical for protein! Clickbait!
I did mention about proteins. No clickbait.
i think its not electrons which the detector collects those are ions! anyone agrees with me?
It's very confusing. If this is wrong, it would have been better to remove it. So many people will develop the wrong concept seeing this.
Why only one electron Kik out sir not other plz explain
why it is known as homogenous magnetic field???
I guess! The strength of magnetic field is same for all the fragment ions so it is homogenous 🤷♂
2018
u are unnecessarily repeating things and making ur videos too boaring. .
u are great teacher sir