Sir..iam a pg chemistry student I have taken notes from ur video...not even touched any other reference..yesterday I have taken seminar on mass instrumentation... I got claps from the lecturer..bcos of ur videos on mass instrumentation and the basics..I think u deserve that...claps
I'm 1 st yr pg student I have no idea about mass spec once I saw ur video ..it's really very clear about mass spec.. thank you sir.. kindly make more videos about instrumentation topics sir
You helped me way more than uni lecturers through undergrad and I still come back to your lectures now in my second year of PhD. Thank you for doing this!!! You’re a Saint.
Thank you so much for sharing your knowledge about MS, I' ve tried to understand what really happens inside the equipment and with your video I could finally get it!!! I coursed a Proteomic discipline during master classes, and I was disappointed because they had to explain the basics first, as you did! As a result, me and my coleagues went out without the most important part of the content! I'm really thankful and I just can't wait to see the other parts!!! Congrats!!! I hope to be a nice teacher as you do!!! Regards from Brazil :-)
I agree a little error in mass is fine!! BUT, come on guys, please do not say molecular weight of H, It is Atomic weight of H =1, molecular weight of Hydrogen (H2) is 2, H is atom, H2 molecule
Before starting this chapter, I thought, I should take small tutorial about this chapter introduction, and i taken help from your video sir, seriously now I can start this chapter happily because you have explained this chapter introduction brilliantly. Thanku sir😊
Sir Really it was awe some presentation. i am mesmerized. got clear. because at this time i am lack of knowledge about GC. When i am surfing about GC accidentally i got into urs presentation. thanks a lot.
Thank you sir! May you live long. You make things so clear. Thousands of students are getting the benefit from your videos. You are so down to earth person, So humble. So simple, so intelligent, so cute 😉 you look like a school going kid, your voice has a different aura. You are making use of social media in most efficient way. We would love to hear some personal things about you like your struggle, your background, these will motivate us. Also just a personal question...Are you married? And what's is your age? Waiting for your reply 😊 Thanks a lot!!
Hats off legend It's really appreciate to watch video No mistakes No extra chutiyapa No hard way Sabse badi baat I seen 4-5 videos on that mass spectrometry Every one say it spectroscopy Except u
this was an excellent video, it helped me thanks. I noticed the incorrect calculations too. I suggest writing an annotation to inform visitors who do not know better of your error. keep up the good job thanks for the videos!!!
Dear Sir, your lecture is full of knowledge thank you very much. I had a question in my mind "How to determine the number of monolayers in graphene using Raman spectroscopy". kindly help me
Thank u so much sir u always help me to study biochemistry topics I'm very thankful to u bcoz without any guidance biochemistry is very big deal to understand
Dear Shomu, how Molecular ion with one positive charge could break into fragments where all of them contain positive charge? it only possible if the molecular ion lose more electrons during the fragmentation. If so, a large number of electrons will be generated in the chamber which might repeal the parent electron required for the bombardment.
Sir, I'm a ug chemistry student.. Your video was useful& extraordinary.. But I wanna Mass spectrum of acetaldehyde video Sir. I search here.. Not here this side..
i think fragmentation is because of the electron bombardment,,,also the peak of the molecular ion will be on farthest right on the graph,,, by this way we can identify parent peak,,
Apart that in your calculation the molecular mass of the alanine would be 96 u and not 94 as you said, you can not use the molecular mass of the hydrogen (approx. 2 u) instead of its atomic mass (approx. 1 u) for the same reason that you have not used the molecular mass of the oxygen (approx. 32 u) but its atomic mass (approx. 16 u) or the molecular mass of the nitrogen (approx. 28 u) but its atomic mass (approx. 14 u). By twicing the mass of hydrogen you wrong the calculation of the molecular mass for glycine and alanine which are instead approx. 75 and 89 u, respectively.
salem allikm,thanks a lot i like your teching method but i am confused about : H mass is 1 because we are speaking about an atom of hydrogen not a hydrogen gaz H2 is not ?
Having a doubt Sir when electrons hit the molecule it develops positive charge but when another electron would come to hit that molecule then it might not get attracted by that positive charge??? And to be get stable why molecules break themselves apart instead of completing it's octate
Hi sir your videos are informative and helpful I'm thank full to you. My request is please do the video on Atomic adsorption spectroscopy. Kindly consider my request sir. Thank you
What happens in mass spectrometry if a peptide contain a large number of basic amino acid? And what happens if the peptide contains large number of acidic amio acid?
I have some 14-day old urine in the refrigerator, I want to drug screen it via GC/MS. Will the results still be valid? What is Forensic protocol, in regards to length of time a sample is valid?
![Oscar Maydon x Fuerza Regida - Tu Boda [Official Video]](http://i.ytimg.com/vi/_ymicn0_GYc/mqdefault.jpg)
Sir..iam a pg chemistry student I have taken notes from ur video...not even touched any other reference..yesterday I have taken seminar on mass instrumentation... I got claps from the lecturer..bcos of ur videos on mass instrumentation and the basics..I think u deserve that...claps
I am really proud of you. Thank you for appreciating my efforts. Thank you.
@@shomusbiologyofficial very useful video sir..I'm also doing 1 st pg on biochemistry....
I'm 1 st yr pg student I have no idea about mass spec once I saw ur video ..it's really very clear about mass spec.. thank you sir.. kindly make more videos about instrumentation topics sir
Sir, yesterday it was exam about mass spectrometry, and I scored 37/40
It totally done for you... sir I love you so much, thank you sir
You helped me way more than uni lecturers through undergrad and I still come back to your lectures now in my second year of PhD. Thank you for doing this!!! You’re a Saint.
I'm a biotechnology masters student and I just wanted to let you know you have helped me so much with your videos, so thank you alotttttt
Thank you so much for appreciating my efforts. I am honored
Thank you so much for sharing your knowledge about MS, I' ve tried to understand what really happens inside the equipment and with your video I could finally get it!!! I coursed a Proteomic discipline during master classes, and I was disappointed because they had to explain the basics first, as you did! As a result, me and my coleagues went out without the most important part of the content! I'm really thankful and I just can't wait to see the other parts!!! Congrats!!! I hope to be a nice teacher as you do!!! Regards from Brazil :-)
2 things for correction-
It should be molecular mass not molecular weight
i spell "peak" wrong. it should be "peak" but not "pick"
sir atomic mass for hydrogen is 1
and molecular mass for glycine is 75g/mol
Good presentation.
I agree a little error in mass is fine!! BUT, come on guys, please do not say molecular weight of H, It is Atomic weight of H =1, molecular weight of Hydrogen (H2) is 2, H is atom, H2 molecule
corrtect 5h=5 only
However sir! Glycine has hydrogen atom not molecules(H2). Thus the molecular weight for 5H should be 5 . Unless it is deuterium instead of protium.
Nice...presentation, dont look into mistakes,,all we know.the atomic weights of elements.the question was either we understand r not....keep it up...
Before starting this chapter, I thought, I should take small tutorial about this chapter introduction, and i taken help from your video sir, seriously now I can start this chapter happily because you have explained this chapter introduction brilliantly. Thanku sir😊
You're welcome. Glad to hear that you're getting benefit from my lectures
Nevermind the crab comments Suman. Those who dont like it don't pliz dont discourage.....Best starting material for newbees
Thank You
Sir Really it was awe some presentation. i am mesmerized. got clear. because at this time i am lack of knowledge about GC. When i am surfing about GC accidentally i got into urs presentation. thanks a lot.
Thank you sir! May you live long.
You make things so clear. Thousands of students are getting the benefit from your videos. You are so down to earth person, So humble. So simple, so intelligent, so cute 😉 you look like a school going kid, your voice has a different aura. You are making use of social media in most efficient way. We would love to hear some personal things about you like your struggle, your background, these will motivate us. Also just a personal question...Are you married? And what's is your age? Waiting for your reply 😊 Thanks a lot!!
Thank you so much for appreciating my efforts
No other videos are best than this. Really very helpful. Thank you so much sir.
Thank you so much for appreciating my efforts
My new favorite tutor!!! Thank you 🙏
Thank you. Glad you liked my lectures
Hydrogen having Atomic mass 1.0079 amu. Overall really nice explanation. It is helpful for understanding the Spectroscopy.
Apart from the calculation part, video was helpful in understanding basic working and mechanism of mass spectrometry.
Hats off legend
It's really appreciate to watch video
No mistakes
No extra chutiyapa
No hard way
Sabse badi baat I seen 4-5 videos on that mass spectrometry
Every one say it spectroscopy
Except u
Thank you
Concept is easily understood, we can visualise and learn, thanku for posting the videos, really helpful in studies👏
You're welcome
Thank you sir .your teaching is excellent. thank you for simplifying the topic .
Thank you so much for appreciating my efforts
Thank you sir ..this video helpful for preaparing tamilnadu FSO exam
You're welcome
Very useful. Ignore the negative comments. You will always find the people spreading a negativity. Stay on positive side.
I like your explanations but in the bigining of this lecture why did you compared X-ray crystallography and NMR with Mas spectrometry ?
Application of X-ray crystallography and NMR applications are different
This guy is very good!! I wish I had seen his tutorials during my university days.
Glad you liked my lectures
Thank u so much for this clearly and interesting explanation! u litteraly explain all I need to know and more !
You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share
Shomu Sir. You are Great ,Greatest
Thank you so much for appreciating my efforts
Thank you sir..i am btech biotech student and i have presentation on mass spectroscopy...
Ur lecture is very helpful for me..
Glad to help you out
@@shomusbiologyofficial plss sir rply
this was an excellent video, it helped me thanks. I noticed the incorrect calculations too. I suggest writing an annotation to inform visitors who do not know better of your error. keep up the good job thanks for the videos!!!
+kate thomas thank you. I will keep that in mind
Thankyou so much Sir... You are really my life saviour :)
You're welcome. Glad to hear that you're getting benefit from my lectures
Dear thanks so much for helping me...
Much love from Pakistan
This will definitely help people. You did a good job. :)
molcular mass of hydrogen is 1.007 only , molecular mass of glycine will be 75.
nice presentation.
Dear Sir, your lecture is full of knowledge thank you very much. I had a question in my mind "How to determine the number of monolayers in graphene using Raman spectroscopy". kindly help me
Thank u so much sir u always help me to study biochemistry topics I'm very thankful to u bcoz without any guidance biochemistry is very big deal to understand
The way you explain is super easy to understand! Thank you for a great lesson :)
+Pi Phong thank you for appreciating
Should make it clear that ion acceleration is induced by an electric field not a magnetic field. The magnetic field deflects the moving ions.
Dear Shomu, how Molecular ion with one positive charge could break into fragments where all of them contain positive charge? it only possible if the molecular ion lose more electrons during the fragmentation. If so, a large number of electrons will be generated in the chamber which might repeal the parent electron required for the bombardment.
Thank u sir u r exaplaination is gud it helps me alot in exams 4rm basic point of view onces again thank u....
Humble correction molecular mass of glycine is 75 and alanine is 89 as the atomic mass of hydrogen is 1. Thank you
Thank you
Sir, I'm a ug chemistry student.. Your video was useful& extraordinary.. But I wanna Mass spectrum of acetaldehyde video Sir. I search here.. Not here this side..
I'm big big fan of urs.. U r one of d best teachers.. 😊
Very very good and complete lecture! Thank you so much.
You're welcome
sir your all spectroscopic parts good , you left onlly one uv visible spectroscopy. so i request you to upload that spectroscopy also.
Thank you so much for all the effort you have put into this! This gives a bit more clarity of the concept.
Thank you so much sir. It was such an excellent video that cleared all my concepts. 🦋🙂
you are just amazing.. God bless you
Thank you
Thank you so much @shomu's biology...
Please solve this question
Q. Interpret Mossbauer spectra of FeSO4, 7H20 from given data
( given , δ=
0.119 aqnd 0.130 cm/sec, Δ EQ= 0.32)
Hey! Your videos are great and helpful. Could you please also make a video on question of mass spectrometry of csir net. It would be really helpful
Okay
i think fragmentation is because of the electron bombardment,,,also the peak of the molecular ion will be on farthest right on the graph,,, by this way we can identify parent peak,,
very nice and very clear - GOD bless you keep it up shamu thanks
Nice video but the molecular weight of hydrogen atom is 1. Molecular weight of glycine is therefore 75.
thank you so much about mass spectrometry basic, yes little errors on example of molecular Mass but it moves
The analytical tool or technique to measure mass to charge ratio of molecules present in a sample is called mass spectrometry
Bro one correction it is mass spectrometry only not spectroscopy, bcoz metry means destructive techniques, scopy means non destructive technique
Hi, you calculated wrongly MWt. For glycine. Glycine Mwt. Is 75 and in your calculation, you consider each H Mwt is 2
Very clear explanation about introduction ...
thnq so much sir ...
Your explanation helped me a lot. Thank you so much!
Mayara Eufrasio hi
KICK ASS HEADSET DUDE! Where did you get them?!?!?!?!?!? Sooooo jealous :/
really good for learners , but little bit changes are to be need.
You helped me a lot.
You're welcome
Awesome.....thanks a lot...plz upload some more videos on analytical bio techniques
Thank u sir... I got an essay i was not sure about the notes... I took notes from your lecture... I passed exam
All the best
You good, you provided the basic tool in understanding mass spec. Thanks for that,,, (y)
u can define it more easily rather than making it complicated but thanks it is also useful
sir ji, i had a doubt regarding molecular weight of hydrogen atom and glycine
Thank you for a very good and simple start
Why negative ions are not detected in this mass spectrometry? Mostly positive ions are used.... what's the reason for this..Sir?
Great explanation.
Thank you
Kindly upload video on TMT (tandem mass tag) techniques for quantification
Thank you so much sir, it's very very helpful to me 🙏💙💙
Apart that in your calculation the molecular mass of the alanine would be 96 u and not 94 as you said, you can not use the molecular mass of the hydrogen (approx. 2 u) instead of its atomic mass (approx. 1 u) for the same reason that you have not used the molecular mass of the oxygen (approx. 32 u) but its atomic mass (approx. 16 u) or the molecular mass of the nitrogen (approx. 28 u) but its atomic mass (approx. 14 u). By twicing the mass of hydrogen you wrong the calculation of the molecular mass for glycine and alanine which are instead approx. 75 and 89 u, respectively.
Thank You for pointing it out. I have not revised it through. I will make the changes.
yes u have pointed the same mistake that i tooo, molecular weight of Hydrogen
Shomu's Biology sir can u make vedio for stability and method validation
salem allikm,thanks a lot i like your teching method but i am confused about :
H mass is 1 because we are speaking about an atom of hydrogen not a hydrogen gaz H2 is not ?
nice presentation i liked it and thank you so much for upload
Perfect! Many Many Thanks Shomu :)
Very good teaching sir..
Thank you so much for appreciating my efforts
Having a doubt Sir when electrons hit the molecule it develops positive charge but when another electron would come to hit that molecule then it might not get attracted by that positive charge???
And to be get stable why molecules break themselves apart instead of completing it's octate
I was talking about Molecular mass.
Very nice explanation...
Thank you
I am really appreciated for the video as it was really helpful for me.
Very good explanation!
Thank you
VERY NICE EXPLANATION>>>THANK YOU FOR THIS VIDEO
Thank you so much for explaining it 💙💙
You're welcome
In time point 14:20, can you explain more about why only 1 electron kick the molecule and why only 1 electron is knocked out， thank you！
Hi sir your videos are informative and helpful I'm thank full to you. My request is please do the video on Atomic adsorption spectroscopy. Kindly consider my request sir. Thank you
Okay. I will try to make a video
Thank you Shomu Bhai. Apnake chara graduate hotam na
Dhonyobaad
Thanks for your lecture
You're welcome
Sir u r the best .
You're welcome
What happens in mass spectrometry if a peptide contain a large number of basic amino acid?
And what happens if the peptide contains large number of acidic amio acid?
Mass spectrometry instrumentation is good...thank u bro..
+Mahadevi c m glad to hear that
Hi
What is the best and portable type of spectrometer which used in analyzing he isotopes in a gas ? same
thanks a lot srr...really its great video for me...
sir m/w of ala= 96 (14+36+14+32)
Your videos iin general are amazing thank you ssssssssssssssssssssooo much
Thanks it was really helpful.
I need to your explanation sir very good job
Good Explanation
You're welcome
I have some 14-day old urine in the refrigerator, I want to drug screen it via GC/MS. Will the results still be valid? What is Forensic protocol, in regards to length of time a sample is valid?
Thank you so much
You're welcome
Is this mass spectrometry playlist is enough for csir net exam? Or anything else is to be covered??
Enough
@@shomusbiologyofficial thanks a ton sir
Can you also please talk about Gas Chromatography please
But in metastable ion video u have told that molecular ion pass through analyser ????