Mass Spectrometry

009 - Mass Spectrometry
In this video Paul Andersen explains how a spectrometer was used to identify the presence of isotopes. This modified Dalton's original atomic theory because atoms of the same element had different masses. The functional parts of a mass spectrometer are detailed including the ionizer, mass analyzer and the detector. A simulation of Chlorine isotopes along with an average atomic mass calculation is included.
Music Attribution
Title: String Theory
Artist: Herman Jolly
sunsetvalley.bandcamp.com/trac...
All of the images are licensed under creative commons and public domain licensing:
"File:John Dalton by Charles Turner.jpg." Wikipedia, the Free Encyclopedia. Accessed August 2, 2013. en.wikipedia.org/wiki/File:Joh....
"File:Myoglobin.png." Wikipedia, the Free Encyclopedia. Accessed August 5, 2013. en.wikipedia.org/wiki/File:My....
"File:Peptide-Figure-Revised.png." Wikipedia, the Free Encyclopedia. Accessed August 5, 2013. en.wikipedia.org/wiki/File:Pe....
File:WidmoMS.gif, n.d. commons.wikimedia.org/wiki/Fil....

[@bullsquid42]

Wow, you've taken something that I assume is incredibly complex, explained it in 8 minutes and I actually understood it.
And I sucked at chemistry.

[@gnuPirate]

+bullsquid42
Don't forget to find a way to test your knowledge and check your understanding, even if you don't like school.

[@bashkillszombies]

Science isn't hard. It, like math, depends on the educator. Most educators suck. Especially people who call themselves 'science communicators' (see: twenty something 'camera ready' girls who are barely undergrad's on buzzfeed like channels who wear t-shirts that read 'i fucking love science' or something along those lines. They're as negligent as the old uppity teacher who thought the class was hopeless and didn't try, because unlike them they assume the entire class is mentally retarded and needs babyspeak.) People like this guy just explain shit the way it is, and if things are explained in a straight forward way there's no reason to not understand them!

[@mustafamalik4211]

He is what we call an actual teacher.

[@blairdent8393]

fr! my chem class online never explained this

[@aakritiramayani5331]

You are such a thorough teacher and what you do for thousands or even millions of students is absolutely amazing. Thank you for all your efforts!

[@sheikhumar7424]

Aakriti Ramayani 'Yeah you are right.'

[@TheJoshtheboss]

Bozeman Science and KhanAcademy are pure blessing. Thank you guys!

[@betsydewey357]

Thank you so much! This was wonderful. I'm writing a paper on identifying biomarkers for pancreatic cancer and specifically researching apolipoprotein A. Thanks so much for going step by step for me to better understand the MS process!

[@khalidbhat9932]

is this a type of biosensor?

[@yansirreaviles5036]

Thank you so much Mr. Andersen!! You are seriously the best. I needed to understand this for my MCAT review and just could not wrap my head around my Kaplan book or AAMC explanations. However, your explanation finally made sense to me. Thank you again, from the bottom of my heart.

[@andrewryan7538]

Thanks, I use this video in my lecture on atomic mass. I like that you showed the calibration of the machine, the whole process makes more sense when you see what can go wrong with analyzing a sample.

[@tmujir955]

The analogy with the car/motorcycle making a turn was brilliant. Thank you!

[@user-xh3nb6gi7o]

wow man you deserve to call you a doctor in your field !
a real person who understand the whole concept and explained it very simple you're not a teacher you're from my point of view a learner

[@nwakolpo]

I wish I had that kind of teacher in university :) Simple and interesting explanation of method sometimes difficult to understand .

[@ilikebands6666]

Thank you so much for this great video! You're great at teaching and I love the fluency in the video and how you explain things. Thanks once again x

[@Guidus125]

what a superb explanation this was, some of these videos are a work of art from an educational standpoint

[@teddystewart9755]

Best video on mass spectrometry i've found. Thanks so much!

[@cavalcantegb]

Sir., you saved me time with this incredible explanation!
Thank you

[@alexatall5405]

Great teacher! Clear, precise and I am SO grateful!

[@zetasky]

Every other video totally confused me with its complexity, but this one totally cleared it all up! Thank you!

[@dirackfield]

Easily the best intoduction to the topic on youtube

[@Friends_72]

You are a god sent. Thank you very much for helping us out. You do really have a great talent in teaching and presenting. So thank you for not keeping it to yourself.

[@calais321]

cheers for the upload, always helpful

[@taylorkayleighhovancik9756]

Can he be my chemistry teacher please?

[@kali6832]

Yes, that was very Helpful. Thank you for making these videos!

[@aofddofa6661]

Thank you for all your efforts!

[@emilyhernandez-avila9081]

Incredibly helpful! Thank you.

[@tannerlynn152]

Awesome and simple explanation. Thank you for making this.

[@TheNeoModd]

this explanation was awesome. thank you for sharing!!

[@fatimaboura2020]

This is so HELPFUL thank you very much !!

[@tharagankumar647]

Such a simple and understandable explanations ! Thank you !!! :)

[@BlazingAngel1]

This was perfect! Thank you so much!!

[@mariansyk]

Thanks

[@paonikos13]

Eleni
An excellent,brief explanation in mass spectometry!

[@PackLIama]

Great video! Greatly improved my geochronology presentation :)

[@StevenMP1993]

Explained thoroughly. Thank you!

[@abhinavporwal1476]

U r a superb teacher........ Doing a great job sir

[@scottyinspiar918]

I can't believe this doesn't cost money! THANKYOU!!!

[@scientistwhosnameisfatima4941]

Oh thx i am 13 yrs old and i decided to learn about mass spectrometry and it really helps me. Thanks again

[@danielamoreno1718]

thank you so much for your help! Great explanation, everything make sense now

[@karolfs94]

I liked this video. Thanks for helping us understand a little better about this.

[@mudfossiluniversity]

Outstanding presentation thank you.

[@TheEpicGod111]

Undeniably the greatest of all time Mr. A.

[@yodaydyxz1752]

Thank you so much for the brief explanation! Helps for sure.

[@waderemington817]

Very clear & concise. Thank you!

[@michaelasvarcova7667]

Really helpful. If you could add more types of ionization that would be absolutely perfect. :)
Thanks to your lectures I have made 3 subjects (two semesters of biochemistry and one semester of molecular biology) :)

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Thank you so much from 2016

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GOAT Science youtuber along with crash course

[@alexanderaugier1976]

Simply put. Very helpful thanks

[@miamiry8737]

Your videos are super helpful, thank you!

[@michellemarie7029]

Thank you:) Best teacher I found for this.

[@danielagaio8090]

I love you! This is clear!!!! Finally!

[@mahamshahid1801]

I couldn't find this explanation in my book so thought Mr Anderson there... who needs the book.. easily explained

[@geethmisandhali3866]

yes,it's really helpful for me in studies.Thank you very much.

[@lukapearson6333]

Great video! I've been going through the series to prepare for a chem test tomorrow, I really like how you explain stuff! Though, for Dalton's theory, wouldn't there also be differences in the size of elements due to differences in ionic radii?

[@Mike-jg9fp]

This video is awesome!, thanks for sharing.

[@marietheresefernandez4636]

Helpen me a lot! Thanks

[@shanisespencer207]

Always helpful .

[@fatimaosman8584]

Very helpful !! thank you very much

[@blakebullwinkel]

Is it only the strength of the electromagnet that is varied to calibrate the device, or can the strength of the electric field also be varied?

[@adhikery2]

Great and very clearing concept....... i see ever......... thanks

[@MariaT-95]

This video is brilliant - thank you!

[@nanuaggarwal4231]

Thank you so much sir.This is really helpful

[@alial-dujaili8633]

thank you so much
extremely helpful
appreciate your efforts

[@lilaclilac1907]

This was so helpful! Thank you! :)

[@galenseilis5971]

I would like more detail about the data analysis for the determination of concentrations of components of a mixture that are exiting a chromatograph. Further discussion on different types of ionization, types of mass analyzers, and finally types of detectors would also interest me.

[@georgesunnyvarkey3425]

Thank you so much sir you have really helped me to sustain my job 😘

[@Zxz1998]

Thank you so much Sir! This is so helpful

[@radkasarastrnadlova6060]

thank you, I appreciate, your biology and chemistry videos a LOT...

[@michaelpappas3857]

excellent explanation!

[@dhyanpatel1469]

Super helpful Thank you!

[@kataroxluvz2rok]

Absolutely fantastic! lets hope this helps me pass my exam

[@Lil.mons19]

THANK U SOO MUCH SIR.... THAT WAS SOO HELPFUL

[@albulenavula536]

fantastic truly helped me with my homework!!!!

[@TheTruebeauty16]

Very helpful. Thank you!

[@hafizabubakar9325]

this video learn me a lot.

[@kellyanderson7624]

I actually understood what you said AND how to get the atomic weight, every other time I felt like I was reading Arabic. LOL. Now I understand it. THANK YOU!!

[@jimlimbarno411]

Cheers boss man, couldn’t have done it with out you 🙏🏼🙏🏼🙏🏼

[@gasperkosmac7672]

Great explanation..thanx man

[@meryemilillou881]

thx men you made my day .

[@ale15stip]

This was super usefull, and i thank you mister!!! I just have 1 question, you said after the magnet curve there is a detector with electron multiplier, but we are only sending Ions down the curve... so does the multiplier, multiply Ions too in order to read/show the peaks?

[@sarahchikho8218]

I needed that video!!!!!!!!!!

[@Ifstarsaligned]

bless you, i have finally understood how this thing works :'))

[@e04ru]

It helps a lot !

[@kaizerthetorus]

Sir, you are amazing!!!

[@elisakarapetyan3700]

Dear Mrs Anderson, would you explain (detailed..:)) how you analyze the single amino acid in the peptide?

[@mursyidahxa]

Thank you! I understand it very well :)

[@ayres3909]

Thank you ( from Brasil)

[@IAMUNDEREIGHTEENYO]

Very helpful.

[@jonBrazilian]

thank you very much! that was very clear

[@rustyjeanz]

Very nice. God Bless you for your teaching. I got a question.So how the abundance was calculated? It has to be in the formula. So what is the way to calculating that. Thanks

[@yosimba2000]

+rustyjeanz Abundance is just the fraction of that specific isotope from all atoms of that element.
So say you have 10 atoms of Chlorine. Of these 10 Chlorine atoms, 7 of them have atomic weight of 35, and 3 of them have atomic weight of 37.
So the abundance of Chlorine 35 is just # of Chlorine 35/ All Chlorine, which is 7/10 = 0.7, which is your abundance.
For Chlorine 37, the abundance is 3/10 = 0.3
Notice the abundance is essentially just the chance of getting that type of atom. You have 0.7, or 70% chance to get Chlorine 35, and 0.3 or 30%, to get Chlorine 37.
Percent chance * Weight + Percent chance * Weight is what is called the Expected Value in statistics, which gives the average value of a Chlorine atom.

[@shalusingh207]

I would also like to know how to read a mass spectrum and how to identify various peaks.

[@kokoro9218]

I love your videos

[@shanewever]

So helpful!!

[@stanleywhitehughes]

Thanks, helping me get ready for a-chem test

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Thank you so much...greetings from Oaxaca, México.

[@pallavi_chandaka9480]

Thank you very much.... !✨

[@toxicated3465]

Thanks Boz❤

[@tux1468]

Gordon doesn't need to hear all this, he's a highly trained professional.

[@alishanshool9670]

Amazing ! May I know what problems faced when mass spectroscopy is used with HPLC system ?

[@jsc3417]

Very helpful!!!

[@onyxash7076]

Yes, that was very helpful :)

[@ditamayo7095]

You're my hero ♡

[@brinnswf]

well done!

[@jameliaancel30]

Very very helpful