How to calculate protein concentration from SDS PAGE Gel using Image J free image analysis tool
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- Опубликовано: 16 сен 2024
- #imageJ #proteinconcentration #SDSPAGE #proteinestimation
This video explains about How to calculate protein concentration from SDS PAGE Gel using Image J.
SDS PAGE is a protein separation technique where in proteins migrate based on the net molecular weight. Using Image J software protein concentration (approximate) can be calculated from the band intensity of proteins.
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Hi.. Thanks for the explaination. For the volume loaded, is it the sample only or it's included with the dye? thanks
thank so much !
Many thanks. However, the subsequent rectangles are returning back and stalking on the first rectangle. What can I do, please?
Thanks... I am at present trying to work with Apo A / Apo B .. would you at anytime going to present a worked example on this topic ? Thanks again for this concise tutorial.
How did you copy the rectangles