1. The sample is added on the stationary phase 2. The mobile phase is added 3. The mobile phases moves through the stationary phase 4. The components in the sample moves with the moving mobile phase based on their affinity towards stationary and mobile phases. THE STORY ❤: At first, the sample is added into the stationary phase. So, at that point, stationary phase is holding the sample. 😊 But as soon as the mobile phase is added, the mobile phase started to run fast pulling the components in the sample along the way through stationary phase👯♀️. So even if they all are standing on the stationary phase, the mobile phase is holding the hand of sample and running forward🥰 until the supply of mobile phase is terminated. But still the mobile phase didn't get his Juliet💔, all the components kept running with him till their affinity lasted stoppedsomewherein between. But sadly there were nobody with him when he ended the race and looked back. Poor mobile phase 😢
The column ? the mobile phase will migrate through the paper and then you need to stop the migration at a certain point to be able to calculate the retention factor ..
You say at 16:16 that mobile phase holds our sample inside, but as you explained later in both paper and column chromtography the stationary phase holds the sample at first. Why is this inconformity? Explain please
1. The sample is added on the stationary phase 2. The mobile phase is added 3. The mobile phases moves through the stationary phase 4. The components in the sample moves with the moving mobile phase based on their affinity towards stationary and mobile phases. THE STORY ❤: At first, the sample is added into the stationary phase. So, at that point, stationary phase is holding the sample. 😊 But as soon as the mobile phase is added, the mobile phase started to run fast and pull the components in the sample along the way through stationary phase👯♀️. So even if they all are standing on the stationary phase, the mobile phase is holding the hand of sample and running forward🥰 until the supply of mobile phase is terminated. But still the mobile phase didn't get his Juliet💔, all the components kept running with him till their affinity lasted. But sadly there were nobody with him when he ended the race and looked back.😢
![GEEK TIMË [OFFICIAL AUDIO]](http://i.ytimg.com/vi/ry-6RvkH880/mqdefault.jpg)
1. The sample is added on the stationary phase
2. The mobile phase is added
3. The mobile phases moves through the stationary phase
4. The components in the sample moves with the moving mobile phase based on their affinity towards stationary and mobile phases.
THE STORY ❤: At first, the sample is added into the stationary phase. So, at that point, stationary phase is holding the sample. 😊
But as soon as the mobile phase is added, the mobile phase started to run fast pulling the components in the sample along the way through stationary phase👯♀️. So even if they all are standing on the stationary phase, the mobile phase is holding the hand of sample and running forward🥰 until the supply of mobile phase is terminated. But still the mobile phase didn't get his Juliet💔, all the components kept running with him till their affinity lasted stoppedsomewherein between. But sadly there were nobody with him when he ended the race and looked back. Poor mobile phase 😢
Very good explanation. Anyone, even though he/she doesn’t have science background, will understand you concept
Thank you for a very understandable and interesting lesson.
Praise be to LORD JESUS
Thank you..good explanation,very helpful......
Very clear video and explainations, thank's !
In paper chromatography why we have to remove the stationary phase before the whole sample pass through the column?
The column ? the mobile phase will migrate through the paper and then you need to stop the migration at a certain point to be able to calculate the retention factor ..
Can you plz make a video on purification by affinity purification.Your videos are very helpfull.
Could you please tell me about the controlled variables in Chromatography?
You say at 16:16 that mobile phase holds our sample inside, but as you explained later in both paper and column chromtography the stationary phase holds the sample at first. Why is this inconformity? Explain please
1. The sample is added on the stationary phase
2. The mobile phase is added
3. The mobile phases moves through the stationary phase
4. The components in the sample moves with the moving mobile phase based on their affinity towards stationary and mobile phases.
THE STORY ❤: At first, the sample is added into the stationary phase. So, at that point, stationary phase is holding the sample. 😊
But as soon as the mobile phase is added, the mobile phase started to run fast and pull the components in the sample along the way through stationary phase👯♀️. So even if they all are standing on the stationary phase, the mobile phase is holding the hand of sample and running forward🥰 until the supply of mobile phase is terminated. But still the mobile phase didn't get his Juliet💔, all the components kept running with him till their affinity lasted. But sadly there were nobody with him when he ended the race and looked back.😢
Kindly review the stationary and mobile phases
i want to know about retention time void volume etc in column chromatography
A trick : watch series on flixzone. Been using it for watching loads of movies during the lockdown.
@Terrance Harper definitely, I've been watching on Flixzone for since december myself :D
Nice concept
Very nice analysis
Hi, Have fine? I wish to you that you have a great day. please explain all types of all chromatography
Your voice is quite awesome
Haha simp
Thank you🙏
Thank you!
nice explanation
Thank you so much!!!!!!
So helpful
Suscribe me bro
Your teaching is good but your accent is confusing
اكو عرب بلطياره🙂😂
Please your English ....
Angrej khi ki
ndak jelas!!!!