Protein Extraction from Cells Part 1

[@gayatrideepti6220]

Very informative video with clear reasoning. One of the best 👍

[@ShakespeareCafe]

These methodology videos are most useful. I watch all of them at 2x speed
All the knowledge in half the time and quickens up structural drawing

[@teppopo6507]

Thank you. Your video are very clearly and easily to understand.

[@indikabandaranayake9432]

very good explanation with basic theories

[@reii9136]

I needed this thank u

[@tomustan]

Who is the instructor? His English is so clear and intelligible, like the schematics/shapes he draws. I am jealous :)

[@helbervalenca]

Cara, muito bom esse vídeo! Eu estava com alguma dúvidas, mas você foi muito esclarecedor! Obrigado!

[@sweatyNate]

This is great thank you!

[@adamkazberuk5310]

Hello John :) Degradative enzymes in lysozymes are active in pH about 4,5-5 due to proton pump activity maintainting proper environment inside of it. In case on lysozyme damage ( for example during the proces of homogenization) this enzymes are not active because of too high pH of cell matrix (about 7,4). Please give me some feedback if You agree or not :) Best regards !!!! :)

[@josemanzano3644]

Very good video although i wish you would have explained the reason why the cells were attached in the wall on the tank in the first place

[@GOSSIPNNEWS2104]

Can u plz tell how can we locate an unknown protein in a cell?? What technique we can use??

[@elR0dry420]

Te amo, haha, gracias por esta gran explicación :D

[@kareshma89]

can we use this method to extract intacellular enzymes from cells and whats the name of the plastic flask u used?

[@aamirahmulla8423]

Hello ... is protein extraction and RNA isolation the same thing ?

[@AdnanKhan-rr2qz]

dear can you contact me bcz i need something about the topic

[@Thomaaasooo]

@@katwil8431 no protein is RNA...

[@bekoemick5892]

when preparing the lysis buffer can one use HCl instead of NaCl

[@ללאכותרת-ט8ל]

use acid instead of salt?

[@tumnekahakaise221]

good video! but man the way u say culture flask!! i had to turn on captions on that... but no, you're fine dw

[@achmadhanifmardinsyah5825]

basicaly this method explain extraction intracellular protein, if u need extracellular maybe this jurnal vvhile help doi: 10.5539/jmbr.w7n1p50