I was browsing around to learn why carbonate buffer extracted proteins cannot be assayed using the more common BCA method. The composition of the BCA reagent presented here seems to give me the answer. Many thanks for this presentation!!
This was so so helpful. Thank you immensely for this, made my introduce to BCA assays so much easier! Now I can write my lab report with slight more confidence!!
hi again :) thanks for your answers, some things on the lowry are still not clear to me, the internet couldnt help, maybe you can: biuret: copper2+ and peptide becomes a complex. we talk about copper II (same as 2+). so this copper can offer 2 places for electrons to the Ns of the peptide. Thats how the complex is built. in the complex copper is still 2+. how does it come, it changes to copper 1+ ? who or what is reducing the copper? and when it became copper 1+, how can it still be in the complex? because in the complex it should always be copper 2+, not copper 1+. thank you very much for your time and your help!
Fantastic work!! Sorry if it’s inappropriate but could I have your last name to reference you work on my lab report? Otherwise I can’t use what I learned here for my work
Amazing! You made Lowry assay on my request. Thank you very much :) Keep it up! please make videos on method and mechanism of Western Blotting technique and Chromatographic techniques.
I saw your comment on the SDS-PAGE video asking for 2D gel electrophoresis, but I could not find it then ... Here is the link: ruclips.net/video/JqFmnxGMc2g/видео.html
i'm working with BCA assay , when i calculate the concentration of protein unknow from the formula of curve , i must multiplie with the dilution factor , how much is the dilution factor in this case and how you have calculated ?? thans to your answer
in lovvry assay.... in frist step you add C(2+), so if there no protein there still molibdenum blue formed right ? because C(2+) unreacted vvith peptide bond can reduce folin reagent
Is fibers proteins which are insoluble in water give this test. Why dipeptides do not give this test. Is it essential to have peptide nitrogens only, terminal amino groups do not form this test. Pl comment. Regards.
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I'm very pleased with this video; You even went so far as to explain why the Lowry method is more sensitive (at around 19:10)
I was browsing around to learn why carbonate buffer extracted proteins cannot be assayed using the more common BCA method. The composition of the BCA reagent presented here seems to give me the answer. Many thanks for this presentation!!
This was so so helpful. Thank you immensely for this, made my introduce to BCA assays so much easier! Now I can write my lab report with slight more confidence!!
Super helpful before our Biuret and BCA Assay lab today. Thanks
Really well explained, please keep on posting!
Great job, you're a gem. Gave me the best refresher I could hope for. Keep doing what you do!
Thank you so mutch .. I will .. and please give me encourage by subscribing the channel and sharing the videos ;)
@@biomedicalandbiologicalsci4989 very helpful, thank you for your efforts. Can you make a video about MSD meso scale?
Thank you please continue your good work
Congratulations on this video! It was extremely useful and very well explained :)
Thank you stay tuned .... :)
This video really helped me alot!!!
Thank you 😁
thank you so much for the video.
Very nice and knowledgable video with easy explanation
thanks, well explained!
Great explanation, thank you!
Loved the way you explained
Great Explanation. Thank you.
Really very helpfu. Thanks Alot
Thank you so much for this video! You helped me a lot understanding this process :)
love your explanation! I completely understand what is going on now! thank you!
Thank you a lot ... Thank you so much for the nice video... I Can understand the reaction
This was the best video ever
THANK YOU!
Thank you so much to share this video, it's very useful for me, I wish you do more video
hi again :) thanks for your answers, some things on the lowry are still not clear to me, the internet couldnt help, maybe you can: biuret: copper2+ and peptide becomes a complex. we talk about copper II (same as 2+). so this copper can offer 2 places for electrons to the Ns of the peptide. Thats how the complex is built. in the complex copper is still 2+. how does it come, it changes to copper 1+ ? who or what is reducing the copper? and when it became copper 1+, how can it still be in the complex? because in the complex it should always be copper 2+, not copper 1+. thank you very much for your time and your help!
Ever find an answer to this?
You explain very well, thanks for the video. New subscriber.
Thank you so much.
Beautifully explained :)
Thank you :D
thank you so much for the video, extremely helpful
Thank you for the encouragement, stay around, new videos are always coming up ..
you literally saved my assignment, it's due today :) I will, lab forever :D
Thank u so much explained in easy manner
Very helpful thank you
Keep making vedio
always appreciated this video thank you so much, really thankful to you....
PERFECT! Thanks a lot!
Great content! What about the phenylalanine? Is it oxidized by the Folin-Ciocalteu reagent also?
Thanks for the explanation 🌸
thanks for a good lesson it is so helpuful
Nice understable lecture❤️
Really very grateful understanding vdyo
Great video!!!
In the Lorry assay: the BSA concentracion to make de calibration curve can be 10mg/ml or we need a lower concentration?
Thank you very much
So fantastic and fascinating video we need more type videos like this I really enjoyed it
Thank you🤗🤗
Awesome 👏
Really a nice video
really helpful
Thank you... it's extremely good and helpful...easy to catch up...
Thank you for your comment .. stay tuned :)
Amazing!
Thank you. It was very clear and useful!
Really helpful. 👏👏👏 Good going. Great speech. Awesome try !
terima kasih bund:)
🥲👍🏻
awesome!
this is an amazing video
it wont stick in my head;-;
Thank you ... stay in the channel many interesting videos are coming up :)
Thanks amazing job .............................
very helpful
Excellent
Fantastic work!! Sorry if it’s inappropriate but could I have your last name to reference you work on my lab report? Otherwise I can’t use what I learned here for my work
Great Video! :)
Amazing! You made Lowry assay on my request. Thank you very much :) Keep it up! please make videos on method and mechanism of Western Blotting technique and Chromatographic techniques.
Ya true this video was made on your request ... and I will try also to make videos on western blotting and chromatography techniques ... stay around
That's so kind of you. Thanks again for such amazingly explanatory videos.
I saw your comment on the SDS-PAGE video asking for 2D gel electrophoresis, but I could not find it then ...
Here is the link:
ruclips.net/video/JqFmnxGMc2g/видео.html
Could anyone explain in short the mechanism of Lowry method ?
super and short explanation but if diagrams was present its very good.....
Thank you for your comment ... and next time I will take care of the diagram issues ..
i'm working with BCA assay , when i calculate the concentration of protein unknow from the formula of curve , i must multiplie with the dilution factor , how much is the dilution factor in this case and how you have calculated ?? thans to your answer
in lovvry assay.... in frist step you add C(2+), so if there no protein there still molibdenum blue formed right ? because C(2+) unreacted vvith peptide bond can reduce folin reagent
18.54 shouldn't the absorbance be at 500nm where the blue colour is in the visible spectrum? instead of 750nm
Our eyes see reflected light off objects, if it absorbed blue we would not see it as blue. The color it absorbs makes the solution seem blue.
Nice vedio
i love your accent
its helpful
Are there any references?
Is fibers proteins which are insoluble in water give this test.
Why dipeptides do not give this test. Is it essential to have peptide nitrogens only, terminal amino groups do not form this test.
Pl comment. Regards.
if the lowry assay remained yellow can u explain why that wouldve happened
❤️❤️❤️❤️❤️❤️
Thank you very much
The video is wonderful and helpful
I want your personal profile if possible
thanks my nigga
Helpful :)
Can we have a sprout seed with protein concentration of 55 mg/ml?
Hello, i want your name is for my university report, so i want to put your name on my references....pls great video
peccato per i sottotitoli in italiano.... tutti sbagliati