How does an ELISpot work?
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- Опубликовано: 4 окт 2024
- Learning objectives:
Explain what problem the ELISpot solves.
Explain how an ELISpot assay works.
Describe the purpose of each of these steps.
Adhesive plastic plate.
Coat the plate in a capture antibody for the cytokine of interest.
Block the plate with bovine albumin.
Incubate cells with stimuli onto the capture antibodies in the plate.
When measuring INF-g, why do we need monocytes and T cells in the PBMCs
Wash step to remove all the cells.
Detection antibody with HRP conjugation
Substrate peroxidation through HRP
Explain why the product of the reaction is insoluble.
Amazing! Thank you very much for this video, it was very clear and super informative. Will be working with this technique in a couple of weeks!
your explanation is amazing. also drawing it out really helps for me to understand things step by step instead of the conventional pictures usually used by lecturers.
Amazing video and great use of graphics
Well done. Beautifully explained.
Really really nice explanation. Simply loved it. I'll never forget it.
This was super clear, thanks a lot!
This is excellent! Thank you!
thank you great explanation