CRISPR-engineered Reporter Cell Lines for Epithelial to Mesenchymal Transition Studies

Epithelial-to-mesenchymal transition (EMT) and its reverse (MET) are physiological mechanisms implicated in tumor cell migration and invasion. To provide researchers with advanced biological models to study metastasis, ATCC scientists used CRISPR/Cas9 genome-editing technology to create a number of EMT and MET reporter cell lines. These reporter lines enable real-time monitoring of the transition of cells from epithelial to mesenchymal state (or the reverse) via the expression of RFP-tagged vimentin or GFP-tagged E-cadherin. In this webinar, ATCC experts will provide an overview of EMT/MET models derived from airway, colorectal, mammary, and pancreatic cell lines and highlight the extensive transition data that was generated in their development and validation.