Suman, You are the best instructor! Your videos are so clear and concise and have helped me to score A's on my molecular bio exams and to articulate myself in my explanations of the various molecular processes. I still return to your videos again and again. Thank you!
Man, you are great educator. These videos are insane, like in my class, we do not go in half the depth that you go into, and I still dont understand anything inclass. Now, i am watching you, go in to so much detail, and I understand more clearly why every step is necessary.
Dr. Shomu's, you are unique, I wish, I were your student. you make me understand many things about plant technology which is my major. I would thank you a thousand times.
I am a student of MSc. (Hons.) Plant Breeding & Genetics, and I have been learning from your videos from the past 4 years. Your way of demonstration is excellent, and It clears my all concepts!
Your videos are Amazing Sir! VERY helpful ... I am doing Bachelors in Medical Bioscience and I watch your vids regularly. They really clear my concepts. THANKYOU so much!! God Bless
I know this video is 4 years old now but sir u helped me through the 4 years in my collage thanks for the help and for always finding what i am looking for in your channel
Hi Suman, You seem very knowledgable about genetic engineering, maybe you can help me. I want to sythesis BDNF from e.coli for some experiments I want to perform on in vitro mouse neurons but I'm not sure where to get the vectors and how to splice in the genes and how to culture and purify the proteins. Can you make a video about it or share some knowledge about how one would go about doing that?
Respected sir, I am doing a humble request that please upload video on biotechnology bparma 6 semister Topic--- Production of Enzymes by microorganisms i.e Amylase, Catalase, Peroxidase, Lipase
I have one thig to ask in this video. suppose after putting the antibiotic resistance gene in this plasmid, this will must grow in media containing antibiotic, but the questions that, if the bacteria is resistance to antibiotic , then how can I be sure that, the desirable gene is also there.
Hey, i know it is a really late reply, but just in case you still want to know why: You usually use a bacterial strain that is not resistant to antibiotics (you can try this by cultivating some wildtype strains WITHOUT plasmid on antibiotic containing plates - they should not grow) Also after you have your plasmid containing bacteria grown on the antibiotic plates, you can sequence them to make sure they also contain the inserted gene
A. Through molecular approaches to study the gene integration. 1. Isolate DNA from transgenic plant & amplify with gene specific primers. 2. Restriction digestion using Restriction enzymes having RE sites flanking the transgene. B. Through event selection we can study the expression of the targeted gene. 1. Southern blotting using probes for hybridization Hope this info will helpful.
I personally think plants strands all has different form of EPSP. Like mouse and human has different antibody, So some strand of plant affected some don’t.
Suman, You are the best instructor! Your videos are so clear and concise and have helped me to score A's on my molecular bio exams and to articulate myself in my explanations of the various molecular processes. I still return to your videos again and again. Thank you!
Man, you are great educator. These videos are insane, like in my class, we do not go in half the depth that you go into, and I still dont understand anything inclass. Now, i am watching you, go in to so much detail, and I understand more clearly why every step is necessary.
Dr. Shomu's, you are unique, I wish, I were your student. you make me understand many things about plant technology which is my major. I would thank you a thousand times.
Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures
YOUR THE BEST DR SHOMUS. U KNOW HOW TO SIMPLYFY COMPLECATED CONTENTS.. YOUR MAKING MY STUDIES EASIER HERE . PLEASE RECEIVE MY FLOWERS
Thank you
I am a student of MSc. (Hons.) Plant Breeding & Genetics, and I have been learning from your videos from the past 4 years. Your way of demonstration is excellent, and It clears my all concepts!
Your videos are Amazing Sir! VERY helpful ... I am doing Bachelors in Medical Bioscience and I watch your vids regularly. They really clear my concepts. THANKYOU so much!! God Bless
Glad to hear that you are getting benefit from the videos. Please subscribe and share
I know this video is 4 years old now but sir u helped me through the 4 years in my collage thanks for the help and for always finding what i am looking for in your channel
+Night CoreS glad to help you for 4 years.
I was about to give up on this chapter, thank you so much sir. You are the best.
Thank you so much for appreciating my efforts
sir you are the best teacher for this subject
you have solve our prob very easily
thankyou sir
You help me a lot...before exam I always remind you and learn from your video lectures... you are awesome bro...love you bro
This video deserves more than 1k likes.
Thank you
Allah pak apko lambi zindagi de ameen
I like your vedios related to biotechnology that helped me a lot
You're welcome. Glad to hear that you're getting benefit from my lectures
U are the best sir, U have explained very very well. 💓💓
Must say u are an excellent teacher!!
Excellent work by you ❤
Thank you
Thank you sir you remain the best you made me understand things in molecular biology
In the application of disarmed Ti plasmid uses any disadvantages present?? Sir please let me know
Thanks a lot this is a very helpful video for my upcoming presentation, you are the best
Wow!! he is giving hearts in a 5yrs old video 😦
i love your videos as always
Thank you
Thankyou sir!Concepts are Crystal clear!
You're welcome. Glad to hear that you're getting benefit from my lectures
Respected Sir I am highly thankfull to you
Sir u r outstanding 🤘
Thank you
U r too good teacher
It is the T-DNA actually transferring (T_R of 24 bp)
Suman I think it is Ti (pronounce: tee I) plasmid.
Apart that useful video for beginners.
Keep going. Doing good job
Thank you
awsum lecture..clear everything
Thank you. Glad you liked my lectures
u r besttttest sir I had learnt a lot from ur vedio lectures ....
thanks sir. you are the best
You're welcome
Hi Suman, You seem very knowledgable about genetic engineering, maybe you can help me. I want to sythesis BDNF from e.coli for some experiments I want to perform on in vitro mouse neurons but I'm not sure where to get the vectors and how to splice in the genes and how to culture and purify the proteins. Can you make a video about it or share some knowledge about how one would go about doing that?
Sir please upload video of plant transformation techniques whole detail abt in a organised manner
Respected sir,
I am doing a humble request that please upload video on biotechnology bparma 6 semister
Topic--- Production of Enzymes by microorganisms i.e Amylase, Catalase, Peroxidase, Lipase
Sir this video was helpful
You're welcome
U are a hero!!!
Thank you
@@shomusbiologyofficial thank you for doing this
I am very interested the this video lectures
You're welcome
Thank you so much sir!😃✌💕
You're welcome
Nice explanation
You're welcome
Thanks a lot sir 🙏
You're welcome. Glad to hear that you're getting benefit from my lectures
Very nice...
You're welcome
Very good sir
You're welcome
Thanks a lot
You're welcome
I have one thig to ask in this video. suppose after putting the antibiotic resistance gene in this plasmid, this will must grow in media containing antibiotic, but the questions that, if the bacteria is resistance to antibiotic , then how can I be sure that, the desirable gene is also there.
Hey, i know it is a really late reply, but just in case you still want to know why:
You usually use a bacterial strain that is not resistant to antibiotics (you can try this by cultivating some wildtype strains WITHOUT plasmid on antibiotic containing plates - they should not grow)
Also after you have your plasmid containing bacteria grown on the antibiotic plates, you can sequence them to make sure they also contain the inserted gene
Can u plz emphasize on biotechnology topics plz!! And some environmental issues too please my exams r going on 3rd yr biotech student can u plz help
thank very much you help me alot
You're welcome
The science behind roundup soybean by monsato?
U didn’t clear, how TDNA transfer to the plant and how u could understand ur desired gene transfer is successful
A. Through molecular approaches to study the gene integration.
1. Isolate DNA from transgenic plant & amplify with gene specific primers.
2. Restriction digestion using Restriction enzymes having RE sites flanking the transgene.
B. Through event selection we can study the expression of the targeted gene.
1. Southern blotting using probes for hybridization
Hope this info will helpful.
And also by gene sequencing for confirmation.
babal smjhaya sir gjab
Thank you
What happens to the wild type EPSP genes in the plants? Those can still get affected by Glyphosate.
I personally think plants strands all has different form of EPSP. Like mouse and human has different antibody, So some strand of plant affected some don’t.
Can you tell me about the EPSP ???
There is no need to consult any book after watching this video
thank u
Can you provide important questions for class 12 boards point of view
I want to be your student...
You can. Watch my RUclips lectures
thanks
😊😊😊😊😊😊😊😊
Sir,, are you a Bengali??
Yes
@@shomusbiologyofficial your pronunciations proved it.. ❤️❤️❤️Best of luck sir... Great video with a stunning concept... 👌👌👌
put the genome of mango tree with mustard oil plants genome then we got mango pickle automatically
😋😋😋😋😋😋😋😋😋😋😋😋😋😌😌😌😋😌😌😌😌😋😋😋😋😋😋😋😋😋😋😋😋😋
What does he say we know the basics of? I can't understand- he says it at 2:20 "...the basic idea of ----------- technology" what is the technology?
genetic recombinant technology
recombinant DNA technology
🙏🙏🙏🙏🙏🙏
You're welcome
Thoda hindi me bola kro
Aree yar. Summery typ vdo bana ra hu bol ke ky bna ya he ree yee ....ek chiz bol ke ar ek chiz ka. Banata hee bee