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BioCoupler Best Practices
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- Опубликовано: 8 мар 2022
- Join Francisco as he breaks down the proper techniques, best practices, and how-tos for using the patent-pending BioCoupler TM!
This innovative and first-of-its-kind device is the answer to the problems and drawbacks of traditional temporary immersion bioreactor systems (TIBs). The patent-pending BioCoupler ™ meets all the basic requirements and accomplishes all the needed tasks for a fraction of the cost of the traditional TIBs, takes up little shelf space in the grow room, and is very easy to use and handle.
There is no need to put your valuable propagules in large vats, tanks, or tubs. With those, the complexity of use and the higher risk of loss if anything goes wrong is too great. Every part can be autoclaved at the standard 121C temperature, or, they can be chemical or gas sterilized if needed.
The BioCoupler ™ allows you to mix, wet, agitate, coat, and move your plant propagules about. It provides high humidity, filtered air, and gas exchange. It does all of this without troublesome plumbing, timers, valves, etc. And, electricity is not required.
We are Plant Cell Technology, a leading organization in the plant tissue culture industry since 1995. We develop, produce, and market innovative solutions for plant tissue culture operations and hobbyists globally. Our well-researched, certified, and trusted products keep your cultures healthy and contaminate-free.
www.plantcelltechnology.com
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When you use biocuplar for philodendron multiplication what is the most suitable amount of the following chemicals
1.BAP
2. Half or full ms
3.sugar
And how many times do we need to turn the biocupler per week? ,
What would happen if I tried TC with a really large explant (like a 6 inch long piece of new, actively growing plant growth on a tropical rainforest cactus)? Do you think this could potentially be used to accelerate plant growth and maturity, if I also never divide it?
Also I bought a biocoupler and can't wait to experiment with it!
6 inches (that is about 15 cm) of the explant is too large. You don't need to use that big explant for the tissue culture procedure. IF you want to use a larger explant then try 2-4 cm. that will work.
hi did u autoclaved this sheet of paper in beginning of the film?
No. you never autoclave a sheet of paper. We just keep the new box of tissue after opening its packet in the TC lab itself to wipe the culture area.
Btw thanks for making these amazing videos they are really helpful 😊
Thanks, Nirosha!! If you are interested in tissue culture, check out our website www.plantcelltechnology.com for any of your tissue culture requirements.
When are you going to do a full cannabis media break down? :)
Thanks for showing your interest in our videos. We're trying to create content on diverse topics and many are lined up to be released in coming weeks. But, we will try our best to create a series on cannabis soon.
However, before that, if you need immediate assistance with your cannabis tissue culture, you can choose PCT's cannabis consulting service to get your answers instantly.
What happened to seed treated with hydrogen peroxide ... Are the results r positive ???
In the same video, you will find that seeds have been transferred to tissue culture and we have obtained tiny shoots growing in each of them. :)
Where do you purchase the Micro Spore filter for replacement for the Bio-coupler at? also what type
Were can I buy biocouplers in South Africa
Hi there,
Please find the distributor closest to your location on this page: www.plantcelltechnology.com/distributors/
Or you can order from our website directly: www.plantcelltechnology.com/pct-store/
What is this in the jaar water or media??
It's media in the jar.
What is the difference between the media used in bio couppler from normal semi solid media( full ms or half MS)?
Full MS and Half MS are different and Semi-solid and liquid media is different.
Full MS and Half MS dictate the strength of the media. half MS would be the use of half the quantity of macro and micronutrients including sucrose for your tissue culture process.
The biocoupler need liquid media (zero agar), while the semi-solid media is a media containing agar concentrations of 0.2 to 0.5%.
Thanks soo much ☺
Hi Francisco! Yesterday I was thinking after all these free lessons that I would order 6 biocouplers from you guys to thank you, unfortunately when I went to basket to pay, it turns out FEDEX defeats the purpose of sending things abroad, I'm in the UK here and 100£ of bio coupler turns into 350£ with postage, is there a way you could send stuff at a normal rate!? 30squids tops, thank you.
Hey,
Try contacting our distributor in the UK with your requirements. Here're the details:
ASR Sciences
Main Office
Manchester, UK
08000 842 543
asrsciences.com
@@PlantCellTechnology excellent thx
Would this be good for beginners? I'm still browsing through your videos and this caught my eye. I did want to ask, what is the liquid used? I was under the impression the liquid is usually thick due to the agar. Thanks!
Hi,
Of course, beginners can also use these.
The media used is normal MS media supplemented with sugar and plant hormones. Do not use agar with Biocouplers, you don't need it.
I recently received my MS media and it's written 5ltr on the side of the bottle,my question is , can I still add up the sugar when preparing the tissue culture media
@@agrippanyagwaya2308 Yes, you will need to add that. check out some videos we have on our channels to best utilize the starter kit.
@@PlantCellTechnology Wy agar is so expensive lke that
@@agrippanyagwaya2308 Are you talking about all agar in general?