Yes good video. The liner to column interface was one of constant wonder for me, whether it seals against the column and such. Some liners make a decent seal, some do not, depends on the liner.
The liner should sit flush against the gold seal.There is a small channel on the surface of the gold seal that allows your carrier gas and the sample to pass through.
The video is very helpful,thanks for that. But what do you mean by , make sure the sample does not exceed the liner, please explain this part . thank you ?
The volatilized sample volume should not exceed the volume of the liner. You might be able to get away with it, as there is additional volume in the inlet. Also, the gas is constantly flowing, if you did inject a large enough volume to force some sample into the gas line, it would just flow right out through the column. You would see it in your chromatogram as a severly overloaded column lol.
Thank you for smart video, I prepare material about Gas Chromatography can I use this video in my material? please if you approve I'll be appreciate for you also I'll mention you page in my material.
that really did help . but, according to how u you defined split ratio that ratio of amount of gas flowing to column to that of flowing to split valve, so in that case higher ratio means higher amount of sample to the column that will surely overload the column and consequentially less accurate results will be produced. ratio must be like, ratio of amount of sample sent to split line to that of sent to column. so in this case higher ratio means small amount of sample sent to column. is this so???
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It is actually simple science. Thank you
Yes good video. The liner to column interface was one of constant wonder for me, whether it seals against the column and such. Some liners make a decent seal, some do not, depends on the liner.
The liner should sit flush against the gold seal.There is a small channel on the surface of the gold seal that allows your carrier gas and the sample to pass through.
This is what Mr. Simple Science...Clearly and easily understood...👍
Content is really helpful.one more suggestion.could you please add sound to video.I mean read them
The video is very helpful,thanks for that. But what do you mean by , make sure the sample does not exceed the liner, please explain this part . thank you ?
The volatilized sample volume should not exceed the volume of the liner. You might be able to get away with it, as there is additional volume in the inlet. Also, the gas is constantly flowing, if you did inject a large enough volume to force some sample into the gas line, it would just flow right out through the column. You would see it in your chromatogram as a severly overloaded column lol.
Thank you very much. It 's a good video.
This is bestest!!!
what is the meaning if i see in the journal he/she uses split-split less injector. is he/she uses split injector/split less injector/ or both?
Really helpful, thank you!
gud explanation..thank u
Great video to understand. But im having very less quantity of sample, which spilt ratio I have to go?
Surely splitless
2:29 shouldn't it be splitflow/column flow?
it worked i my exams!!
cool
Thank you for smart video, I prepare material about Gas Chromatography can I use this video in my material?
please if you approve I'll be appreciate for you also I'll mention you page in my material.
Tremendous one!!
Well done..👍
that really did help . but, according to how u you defined split ratio that ratio of amount of gas flowing to column to that of flowing to split valve, so in that case higher ratio means higher amount of sample to the column that will surely overload the column and consequentially less accurate results will be produced. ratio must be like, ratio of amount of sample sent to split line to that of sent to column. so in this case higher ratio means small amount of sample sent to column. is this so???
higher ratio will mean more of the sample will go out to the split than into the column. This will result in smaller peaks
Clear explanation!!! Thank a lot
I thougt my computer sound settings were off lol
Really very helpfull..
Why do we use mostly Split injection in GC?
1. To prevent overloading the column. 2. In some cases, it produces sharper more well define peaks
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