Lecture 32 Isolation and Purification of Proteins

Protein purification, IPTG, cell pellet, lysis buffer, FPLC system, PMSF, lysozyme, sonication, Ni-NTA column, BME, SDS-PAGE

[@alokcpradhan]

Can't believe so much information of soo much value this video offers!

[@priyanairrai1462]

Very comprehensive and methodical presentation. Kudos to the presenter.

[@monapopa7493]

Very interesting and well done. An excellent education material for our students! Thanks a lot.

[@seharfazal5070]

A great work indeed!!! Too much valuable information in a single video... Carry on this work please... My first ever comment on you tube... You just compelled me for appreciatiom. Many Thanks

[@parijatdas2822]

This is the excellent, could you please make a video on mammalian expression systems eg. CHO-S and HEK293T cell lines.

[@tmanbravo]

This is a awesome video , very helpful. Great demonstration. Accurate protein purification demonstration

[@ChitunduCharles-ef8jj]

Helpful video 😊

[@Tv7_telugu]

Amazing teaching madam, thank you so...much

[@subhasreevaradarajan9895]

very helpful and informative video maam.. i have one question ..what if the ice melts ? is the temperature maintained inside is cold ? or what kind of ice should we use maam ?

[@ranjitkumarpanda128]

It helped me a lot mam for my protein estimation.

[@odishagalaxy5641]

Thank you mam this is a best valuable video mam please make more videos like this so that it will be helpful for different students on the field of research.

[@anushkapundir6410]

Thankyou so much . Cleared all the doubts .

[@pravinayadav294]

great video

[@pramilayadav8010]

Well explained....nice

[@foodtechnologist838]

What will be the effect of ultrasonication and centrifugation on the fat content in the sample.
I want to remove only protein from my sample and keep my fat inside the sample..is it OK to use these processes to keep fat in the sample?

[@santamsaha9415]

Great video...

[@Sing-with-Amna]

Can you tell me how much quantity of lysis buffer you have used for resuspension the cell pallet

[@andressalgadomartinez6871]

Nice video! Which protein did you purify?

[@akwangyoshuapatasik3080]

Sangat mudah dipahami 🙏

[@monikayadav866]

Really helpful. Thankyou.

[@Pluschronicle]

its all useful for gate xl

[@dipalimalvekar2472]

How we extract protein from animal tissue pls guide

[@rohitkr7637]

Thank you mam

[@faanraja4755]

nice method

[@gurralamohankumar5838]

Thank u so muchhhhhh

[@konesironald9575]

She is pretty

[@priyaselvam764]

What is meant by PI ?

[@himanshupanda1690]

Isoelectric Point...At which pH the net charge of the protein becomes ZERO

[@shardabharti2091]

Isoelectric point

[@biryani6861]

madam can you share the email please I want to discuss about my samples. Thank you

[@alphonsraia3523]

Aduthathada pls kojam tamil la soluga

[@tamilbiology5078]

It's a central university bro tamil ah sollamaatanga

[@rajavishnuvardhana6830]

She is not talking about Sangam literature here...