RNA interference (RNAi) Animation || miRNA || siRNA || mRNA regulation
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- Опубликовано: 22 май 2021
- #biologyanimation #RNAinterference #siRNA #miRNA
This animation describes the history and discovery of RNAi along with a detailed gene silencing mechanism by mi RNA and si RNA. This also describes the processing of miRNA. Different mechanisms of mRNA
regulations are mentions and explained in this animation.
Gene silencing mechanisms of siRNA and miRNA.
siRNA: dsRNA (either transcribed or artificially introduced) is processed by Dicer into siRNA which is loaded into the RISC. AGO2, which is a component of RISC, cleaves the passenger strand of siRNA. The guide strand then guides the active RISC to the target mRNA. The full complementary binding between the guide strand of siRNA and the target mRNA leads to the cleavage of mRNA.
miRNA: Transcription of miRNA gene is carried out by RNA polymerase II in the nucleus to give pri-miRNA, which is then cleaved by Drosha to form pre-miRNA. The pre-miRNA is transported by Exportin 5 to the cytoplasm where it is processed by Dicer into miRNA. The miRNA is loaded into the RISC where the passenger strand is discarded, and the miRISC is guided by the remaining guide strand to the target mRNA through partially complementary binding. The target mRNA is inhibited via translational repression, degradation or cleavage. 
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I watched at least 15 videos but nobody explained it as clear as you did. Thank you so much🙏🏻
Thank you so so much 🥰 keep supporting
Same here mam
You don't know how much it helped me ... THANKYOU SO MUCH 🙏🏻🙏🏻 ..
such a clear explanation
Absolutely amazing. Thankyou 🙂
Great.... !
literally slay thank u so much
very nice
Amazing 🤩,thanku so much
The best explanation video ever, so clear and complete! Thank you so muchh 🙏🙏🙏
Thanks a lot to you too dear 😊
Thank you, thank you!
It was the best and simplest explaiination of RNAi , thank you 😊
That's a great honour
Your way of explaining the concepts is very good.
Thanks a lot
Best way of explanation 👍
Thank you so much 😇
Thank you! Keep supporting!
Great, I really appreciate it. You explained it very well 👍🏻
Glad you liked it
Thanks for your efforts👏👏👏
Thank you 😊
good video :)
Superb
Thanks a lot, please share if you liked our videos
Tq so much , I enjoyed watching this 😍😍❤❤
So glad!
Mam I have a doubt in which condition miRNA and siRNA will gets activated
عاش اميتاب باتشان و الله تاليها كمنا نفهم على الهنود 😅
Isn't AGO1 for miRNA ?
Hey can you tell me about RITS comples
The RITS (RNA-induced initiation of transcriptional gene silencing) complex contains siRNAs and is the major effector of RNA interference. Heterochromatin assembly is associated with large-scale chromatin condensation to regulate a variety of chromosomal processes, such as centromere formation.
Below is the link for a Cell article, you may find useful. We will try to make a video on that too.
www.cell.com/molecular-cell/pdf/S1097-2765(04)00057-7.pdf
@@RethinkBiology thanks
How to apply in plant.. ?
RNA interference is a natural process that plants use to regulate gene expression and defend against viruses and other pathogens. It involves the production of small RNA molecules that bind to specific messenger RNA (mRNA) targets and prevent them from being translated into proteins. There are different types of small RNAs, such as microRNAs (miRNAs) and small interfering RNAs (siRNAs), that have different origins and functions in plants.
To apply RNA interference in plants, researchers can use various methods to introduce small RNAs or their precursors into plant cells. For example, they can use genetic engineering to insert DNA sequences that encode for small RNAs or hairpin RNAs (hpRNAs) into the plant genome. These sequences can be driven by constitutive or inducible promoters to produce small RNAs in a specific tissue or time. Alternatively, they can use viral vectors to deliver small RNAs or hpRNAs into plant cells through infection. These vectors can be modified to reduce their pathogenicity and increase their specificity. Another method is to use spray-induced gene silencing (SIGS), which involves spraying synthetic small RNAs or hpRNAs onto plant leaves. These molecules can enter the plant cells through natural openings or wounds and trigger RNA interference.
The applications of RNA interference in plants are diverse and promising. RNA interference can be used to study gene function, improve crop traits, enhance stress tolerance, and control pests and diseases. For example, RNA interference can be used to silence genes that are involved in flowering, fruit ripening, seed development, nutrient uptake, photosynthesis, and hormone signaling. This can help researchers understand the roles of these genes and create plants with desirable characteristics. RNA interference can also be used to silence genes that are essential for the survival or virulence of plant pathogens, such as viruses, bacteria, fungi, nematodes, and insects. This can help plants resist infections and reduce crop losses.
If you want to learn more about RNA interference in plants, you can check out these sources:
(1) RNA Interference Technology in Plants: Mechanisms and ... - Springer. link.springer.com/chapter/10.1007/978-981-16-6974-3_10.
(2) RNA Interference Past and Future Applications in Plants - MDPI. www.mdpi.com/1422-0067/24/11/9755.
(3) RNA Interference- Definition, Mechanism & Applications - BYJU'S. byjus.com/biology/rna-interference/.
(4) Exploiting RNA Interference Mechanism in Plants for Disease ... - Springer. link.springer.com/chapter/10.1007/978-3-030-35955-3_10.
(5) RNAi: What is its position in agriculture? | Journal of Pest Science. link.springer.com/article/10.1007/s10340-020-01238-2.
Are you bengali?
Better not to add music!
See our latest videos, we don't add music 😬
Background music is disgusting
Visit our newest videos with no background music
It was a good choice of music, so be quiet jit.