Lentivirus Titration: How to Determine Viral Titer

So, you've harvested your lentiviruses and are ready to infect your cells! Hold on a minute - do you know how many lentiviral particles you have? It's time to calculate your lentivirus titer!
In this fourth video of our lentivirus series we'll show you various methods for determining viral titer. This video covers:
• Functional vs. Physical Titer
• How to determine functional titer: FACS, Colony Formation Assay, qPCR
• How to determine physical titer: p24 ELISA, qRT-PCR
• Which titering method you should use
• How to calculate titer
There are two different types of titer measurements: functional and physical titer. Functional titer measures how many viral particles are actually able to infect cells, whereas physical titer simply measures how many total viral particles there are, both functional and non-functional.
Calculating viral titer is a foundational skill for every cell biology lab and we hope our video helps you plan your own viral titering experiment with confidence!
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[@lucierobert9151]

Thank you so much, this video is wonderful !

[@abmgood]

Thank you for watching! Glad this helps!

[@swatianand3227]

Mam kindly make some more videos regarding recombinant DNA techniques.. I found it very useful to understand and learn in such a simple way.

[@abmgood]

Hello Swati,
We are working on the next video, "Lentivirus Infection"! Stay tuned ;)

[@anwarullah5017]

very good useful information a sample way for understanding

[@abmgood]

Thank you! Glad you found the video helpful! :)

[@JYOtiRaNJanMANgaRaj]

❤❤❤❤❤❤

[@adzralalamsyah9913]

how about the TCID50 and the plaque assay?

[@abmgood]

TCID50 often takes several days to complete. For researchers who are looking for a faster method, oftentimes qPCR is considered as it can be completed in one day. As for Plaque assays, these are typically used for viruses that form plaques, such as adenoviruses.