This Machine Grows Living Flesh 🥩
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- Опубликовано: 26 июн 2024
- This amazing machine allows scientists to grow animal cells.... outside of an animal. Today we explore how we built it, how it works, and then take it for a test drive by growing some mouse fibroblast cells.
Github: github.com/thethoughtemporium...
Andrew Pelling's incubator: www.pellinglab.net/post/diy-i...
Previous videos:
Meat berry - • A Grape Made of... Mea...
Neuron project 1 - • Growing Human Neurons ...
Neuron stream - ruclips.net/user/livep1C0qpqpAWc?...
Follow up videos:
Meat Leaf - • A Leaf Made of... Meat??
Can Neurons Play Doom? - • Growing Living Rat Neu...
0:00 Intro
0:41 Previous Projects
2:00 Building the incubator
5:00 Choosing Cells
6:41 Growing Cells
12:00 Future Outlook
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At this point, you are only a few steps away from going full mad scientist.
He already is
El Psy Congroo
you mean a few steps past
Considering that he's done gene therapy on himself to temporarily cure his lactose intolerance... "few steps away" is correct, but the boundary is backwards, not forwards
I think he's already past that
Sweet! Man-made horrors beyond my comprehension
Time to make the mystery flesh pit superorganism
@@jorge-052 Yay ! New Disneyland attraction !
Resident evil time
I can comprehend these man-made horrors perfectly fine, must be a skill issue or something.
I mean, it's just cells, it isn't really "alive" as in conscious
"if you thought the meat berry was weird, wait until you see what we do with a slice of bread"
That has to be a brand new sentence
this entire video is a brand new sentence for me. definitely interesting though
Bro I am scared he would make meat wood 💀💀💀💀
Nah man nothing's wrong with that he just making meatloaf
@@tristanellis7570 lmao
That's just meatloaf at this point.
I used to work in a lab where I grew neurons in culture from zebrafish I dissected and I have some recommendations if that's chill. In your video about growing neurons, I saw that you used Neurobasal-A. I can definitely recommend Neurobasal Plus instead. They both work, but the latter has given much better results. Also definitely coat whatever glass you're using for the MEA in PDL so the neurons can adhere to the surface. I'd also be happy to share more detailed protocols or tips and tricks if you want :)
neurobasal plus +b27 is what I'm looking at for the neurons. Plus PDL coated cover slips in a 12 well to start with. For the actual arrays it'll be basically the same, but directly on the array.
@@thethoughtemporium yeah cover slips in a 12 well is exactly what we did as well haha. Multichannel systems also has good resources for MEA related things. Best of luck!
So this is what it's like for my mother when I start talking about feruchemy...
@@aidengray3998yeah lol
@@aidengray3998I'm just here just pretending to understand the conversation like "hmm yes neurobasal"😂
I would imagine you already have them, but it's good to have one or two co2/oxygen alarms in the lab in case of a leak.
ya we've got one near by. Thus far, having 2 people just hanging out in the room raises the co2 much more than the incubator does.
@@thethoughtemporium How many people do you have on the team? Are you the sole scientist working on electronics, code & biochem or is there team & community effort?. These videos are so cool!.
We've got a small team of about 5 now
@@thethoughtemporium That's cool, I love all 3 domains. Projects & experiments are only a fantasy in my country. Do you guys publish any papers or is it all for youtube?
CO2 aint as dangerous as other oxygen deficient atmospheres caused by heavy gas because you should notice the shortness of breath which is more than you can say for other common suffocating or poisonous gases. Shoddily plumbed CO2 is pretty common in hospitality work for carbonating/ pressurising liquids and often in the most confined spaces (walk in fridges and floor hatches, now I'm not for low safety standards in the workplace but I have never heard of anyone dying to a CO2 leak despite a mild interest in hazardous atmospheres.
We are doing exactly this at the cancer institute I work at to attempt to replace malignant tissue in situ. Couple that with the targeted mRNA genetic medicines that we can now build in about three hours specifically for the person- well, point is the real shit is happening and I adore scientists like yourself pushing the boundaries to make what was prohibitively expensive just ten years ago almost cheaper than a burger and fries. Cheers and keep on, mate.
P.S. As an aside I too was extremely disappointed about the lack of eagle involvement in DMEM. "Oh shit!? We synthesized a medical medium from an eagle!? What'd they have that we didn't!? ... ... Oh. Oh it's just the name? ... Oh. No that's fine. I just... Nevermind."
"From the creators of Baby Cow Juice, here's Baby Eagle Jelly! Available now"
Oh, yeah, that "technology" of died suddenly. Sounds fantastic
@@UniqueBreakfastTaco What are you talking about? More like the technology of one of my patients with pancreatic ductal adenocarcinoma (which was an absolute death sentence in just 2010) is at home, alive, and looking pretty damn good. That's "Alive suddenly."
@@cwtrain Hes an anti vax moron :)
Three hours sounds almost unbelievable! How do you develop a targeted mRNA so quickly? We have an ilumina at our university and I don't think I could even get a bacterial genome sequence back in 3 hours. What does the mRNA code for? Our iGEM team is narrowing down projects, and this seems really cool (albeit difficult from a wetlab point of view, I've heard nothing but horror stories about RNA work)
The thing I like most about this channel is that everything is so well explained without being too technical for people without specialized knowledge to understand. Finally, man-made horrors that are within my comprehension!
just a quick comment, you should pipet slowly up and down once the freshly thawed cells to resuspend them, then add them to a clean tube/container and slowly add previously warmed media on top (drop by drop at the beginning). I know fibroblast cell lines are quite sturdy, but it would still improve your survival rate and it's good practice. It prevents osmotic shock that can kill a lot of your cells. Also, if you try to stay sterile, dumping liquid from any container is not a good idea (you always assume the lip of the container is not sterile), plus you're probably throwing away the last drop 50-100ul of liquid left in the vial.
On another note, I like your incubator, we just had to buy a new one in my lab and those are quite expensive.
"What I assume are your most obvious questions."
Where can I get one!
"WHYYYYYYYYYYYYYYYY"
Oh...
I took a look at the code and I note that the co2 injection code does not account for humidity. I suppose that if you are keeping the humidity constant it shouldn't matter that much, but AFAIK co2 sensors are quite sensitive to humidity. If you run into problems with the co2 concentration, that might be something to keep in mind. Keep in mind I am a software/firmware developer, and I don't know very much about biotech, so it might not be a problem for you. Very cool stuff!
Relative humidity is kept at nearly 100% in most cell incubators and doesn't vary much unless the incubator has been opened recently and hasn't normalized yet. He has a bowl of water on the bottom also being warmed by the incubator, so it's keeping the incubator humidity as high as possible. So as long as the sensor is calibrated to read at saturated humidity it will be fine. The cells need the humidity too, so if the bowl dries up the CO2 will be the least of your worries.
I'd want one of these whenever I'm at.
Started my research project for my final year, working in the tissue culture lab is damn awesome. We’re literally using the same stuff minus the cell line (HEK 293T cells) and the buffer (PBS I think). Thank you for inspiring me to carry on no regrets at all. All the best for this year, fingers crossed that nothing gets contaminated.
I love your channel so much, very excited to see where this journey progresses and glad that you're not only interested in documenting the process through your channel, but also entertaining and good at making videos.
As a former cell technician during my university years, the thought of having bacteria incubating right next to mammalian cells is worrisome. Contamination will become a huge headache! Absolutely love these projects. Please keep inspiring people and showing them that anything is possible!
Could you use recombinant bacteria to make growth factors and have them grow together with the mammalian cells?
@Docteur Livesey Why food of all things? If you want food, make funghi.
@Docteur Livesey terrible logic. No one is making you eat lab meat but "what if theres some unknown risk" isnt much reason to be deterred. That risk will always be nonzero, appealing to that fear is irrational.
@@SianaGearz I want to consume the living flesh. I hunger!
@@retroicdescent Have you tried frog legs?
"I've got a freezer full of mice meat" brilliant
If I had heard this from anyone else, I would have assumed they were a serial killer lol
If you've ever had a pet snake this is a normal sentence
@@neb_setabed why would a serial killer have mice meat?? 🤣
@@Jehty_ To lure in their victims, duh...
@@Jehty_ Because the serial killer is a cat, duh!
question: in the future, will I be able to "grow" a steak consisting of my own cells and safely cook and eat it?
Yes, but it's a bit like saying "could I build a rocket and put myself into space". If you're a rocket scientist with space agency resources then maybe yes, otherwise very difficult.
He's also not actually growing meat here, fibroblasts aren't muscle cells and they're much more forgiving to grow.
@@CatHatOwl I suppose I could've worded that a bit better, I was more just asking about the technology rather than specifically myself doing that, though I do want to eat myself, I think that'd be cool
@@canadiangopnik7007the technology honestly is probably possible now. Although its probably very expensive and not worth it.
@@mqegg nonsense, the clout I'd get from being able to say that I've eaten myself with 100% honesty is priceless
@@canadiangopnik7007personally I wondered about human... why am I so weird,_,
GODDAMNIT FINALLY FINALLY finally a new proper video! Thankyou
Been waiting way too long
Looking forward to this journey.
how the hell did you comment before the video came out?
Patrons see videos first
@@thethoughtemporium do they also get dibs on the first morsels of mouse flesh?
Maybe.
@@thethoughtemporium if this was any other RUclipsr I would be sure this is a joke, but with you I can't tell
This may be a silly question, but I see you're dipping some of the items in a ultrasonic cleaner. Is that just to clean it or for something else?
Love your videos! Thanks for all the amazing work!
it's got a hot water bath function, so that's all we're using it for. The ultrasound is off
@@thethoughtemporium A bath with an ultra surround sound? Now that's posh. /s
Reminds me of my time working in an oncology lab. Contamination was always something to pay attention, as even the smallest speck of dust is enough to ruin a dish.
I wonder how the cells will grow on different surfaces like copper and gold (not sure if they’re anti microbial), and how they will adhere.
Good luck and keep up the good work! It’s been a lot of fun watching the projects over the years. Thank you!
I remember watching each of your videos as they came out one by one. Your meat video came out on my Sophmore year of College and now this is out just months before my graduation walk! It's so cool to see you here now! I'm so jealous of all the cool things you're doing and hope I can do these kinds of cool things someday!
It's nice to see you haven't abandoned the neuron-computer project!
It's been so long! I can't put ot words how excited I am to see what you come up with! So many people hate it, but genetic modification is truly the most exciting and bleeding-edge field of biology (and I would argue all other sciences spare maybe computer science right now) out there!
I just today discovered this channel… the sophisticated projects you are pulling off are mind blowing
Really looking forward to all these new projects!
I did also a mammalian cell culture at my workplace, but we made us things a little bit easier.
We measured only the time (about 20 seconds) when we added trypsidin so we didn’t need to take it out of the fume-hood-thingy (I don’t know how it’s called in English).
After the 20 seconds we removed all of the liquid, and waited about 5 minutes. There was still enough trypsin in the thin film of liquid sticking to the cells. We checked if the cells where ready, by gently tapping the flask on the table, when there where visible smears appearing, it’s ready to continue. After that we just added new FCS and all that other stuff, the trypsin after that was diluted enough to don’t matter anymore, counted the cell concentration in a neubauer counting chamber, calculated how much wen needed to transfer into a new flask and we where done.
(Sorry when I have some names wrong, I don’t live in a English speaking country)
The sentence "This machine grows living flesh" is scary to think about
Great to see progress towards revisiting the neuron project
Stumbled on your shorts, and quickly subscribed and now starting at the top (newest as of this post) and working through your videos... good stuff!! Thank you keep them coming
I am very interested in learning about alternatives to fetal bovine serum. I was under the impression that there aren't any viable substitutes? Looking forward to maybe a dedicated small video on the topic? Might give the whole industry a push if awareness is spread from somewhere
Surprisingly, magnetic fields!
Back in November 2022, a research team in Singapore’s National University of Sciences managed to use magnetic fields to stimulate the release of growth factors by myogenic stem cells to accelerate the growth of meat/muscle tissues, all with no FBS.
They’ve already looked into commercializing this technique/technology for use in regenerative medicine and cultured meat production.
@@serinahighcomasi2248 On a more practical side... use human platelet lysate from buffy coats. It is a commercial product.
From what I've heard there are alternatives on market today they just aren't as effective. Shockingly I cannot speak on this subject with any first-hand experience as I have nowhere near the money to perform these kinds of experiments at all.
The cultivated animal tissue (CAT) burger industry is trying desperately to find an alternative to baby cow juice so they can make actually vegan CAT meat. I don't know why nobody has taken up this obvious acronym. Replacing animal slaughter products is their main USP, they would not have many shareholders otherwise. As far as I know, they haven't got very far yet.
@@serinahighcomasi2248 Interesting that it is antagonised by streptomycin. They may have to find more tractable antibiotics, that can also be removed easily from the final product. Has anybody tried oregano? Craig Jun Kit Wong _et al_ "Brief exposure to directionally-specific pulsed electromagnetic fields stimulates extracellular vesicle release and is antagonized by streptomycin: A potential regenerative medicine and food industry paradigm" _Biomaterials_ Volume 287, August 2022, 121658
Disappointedly doesn't contains eagles
Excited to see a new vid! Love to see more, it’s clear your channel is the real deal
I love the dedication to awesome projects from this channel!!!!
I have an improvement idea for your electrode array. You can buy "Empty" IC Packages.
Pins are Gold coated, can have 8 to 64+ pins, availabe in any form from SMD,DIP, to a huge cpu package AND are sterile. Also you will get a solid connection.
I saw the epoxy and gold coatings fail in the original video, so i think this may be a nice upgrade.
One thing I've always wanted to try if I had a lab is to get a mold of some fossil and grow bone cells in it, making a "real" dinosaur bone
Great video as always, looking forward for more!
I’d imagine it to be quite hard to give a mold-grown bone the necessary stability to appear bone-like. Physiologically (in mammals like humans at least and probably a lot of other animal species) most bony structures actually start out cartilaginous and slowly ossify from ossification centres following the natural stress vectors of the bone which you’d be lacking if you were to try to grow everything in a mold
I do appreciate the effort put into the looks of the Meatcubator.
Welcome back!!! Very excited to see what you’ve come up with
$1k for a flask of cells? What the hell am I doing with my life??
You forgot to mention just how expensive FBS is! I've done a lot of tissue culture myself and seeing the recent developments in cultured meat has got me wondering about the "FBS question", since using FBS isn't that conducive to a cheap or cruelty-free product. I'll be super interested to see if you're able to get good results using serum-free media.
Magnetic fields. No, seriously.
Back in November 2022, a Singapore research team managed growth factor production from myogenic stem cells using pulsed magnetic fields.
@@serinahighcomasi2248 Craig Jun Kit Wong _et al_ "Brief exposure to directionally-specific pulsed electromagnetic fields stimulates extracellular vesicle release and is antagonized by streptomycin: A potential regenerative medicine and food industry paradigm" _Biomaterials_ Volume 287, August 2022, 121658
There certainly are plenty of alternatives, but the issue is the alternatives are even more expensive right now. The biggest one is Knockout Serum Replacement, which is a chemically defined serum-like fluid, though it misses many growth factors. It's quite expensive, more than serum, and can't replace it in all situations. There are also specialty medias for for a ton of different cell types that are serum free, Essential 8 for pluripotent stem cells, Serum Free Expansion Media (SFEM) for hematopoietic cells, etc. It's an eventually solvable problem for the industry, but it's not always done right now because of the cost of the alternatives or the necessary growth conditions just aren't known yet for a particular cell type. Cells can also be genetically engineered to express their own growth factors or transcription factors that preserve their cell state and proliferative potential.
Growing animal cells is a completely new level of development for your channel. Thank you for all the content you post freely.
ayy, been waiting forever for a new full length video, glad its here
Quick question: how do you store the fibroblasts for long term use? Do you have a liquid nitrogen dewar or a -80? Also, do you freeze them gradually with a Mr. Frosty (-1C gradual freezing)? I’m amazed at what you’ve been able to put together without being too much at the mercy of thermofisher, or sigma!
Which C02 sensor did you go with? I've been trying to pick one that uses I2C but pretty much every one I've found has a lifespan of 16-24 months and aren't cheap... 😬🤦
If you have a/any suggestions I'd greatly appreciate it bud 🙂
Really happy to see a new video of yours up, today's been a good day!
Oh same, want to monitor my office
Finally, another upload. Excited to see a nice and straight forward video on the channel, even if it isn't the sequel to the opal video that I happily await.
YYEEESSSSS, FINALLY!!!!! I've waited so long for you to post again.
Man, I wish I was mammalian cells
you wot ?
@@foobar879 *screeching Eldridge noises*
Good news !! If you're human, you are both a mammal AND cells !!! That's so swag !!
You aren't?
This is incredible.
So excited to see where this project goes!
Just starting this video and I have to turn the volume up all the way to hear you. But other than that I love your videos thank you 👍
Hey Thought-emporium! I remember that you and some guys did a video a few years back growing Kombucha SCOBY as a "Vegan-Leather".
After advancing and refining your process of growing Mammalian Cells, do you think you could Re-celularize the SCOBY leather? Would be interesting to see how much the tissue itself is Bio-compatible since it is, in theory, just cellulose... Who knows, perhaps it could even become a "bio-compatible canvas" to grow shaped meat and other abominations!
Have you seen the work that Micheal Levin is doing with computational biology? I'm curious if any of the techniques he is exploring are applicable to what you are working on - no idea how it would apply, but it seems vaguely related. I'm really enjoying seeing how you've progressed!
I do TC every day at work, but I’m still amazed by how you’ve put all this together and your insight
Happy to have you back! Hope all is well
But can it grow sausage
Would a humidity sensor be a good idea to install into the meatcubatorfor logging purposes?
Nice, new incubators have them, but it's not completely necessary. As long as he keeps the water bowl on the bottom filled, the humidity will saturate. It's easy enough to do a circuit that alarms if water doesn't bridge two electrical contacts to remind you to fill the bowl.
bro i was literally wondering when you were gonna upload yesterday, this is great!
Awesome! The neuron project was one of my favorites. It is nice to see progress towards it. BTW I'm still waiting for updates on the spider silk project.
Ahh, yes...
Dr. Doofenschmirtz's brand new _"Make Vita Carnis a Reality-inator"_
Why do mammalian cells need CO2 to grow/survive?
Googles answer:
"The purpose of CO2 in cell culture is to maintain a stable physiological pH through the CO2-bicarbonate based buffer system. The atmospheric CO2 can dissolve into cell culture medium, and a small portion of it will react with water to form carbonic acid, which in turn interacts with the bicarbonate ion. The balance of dissolved CO2 and bicarbonate thereby controls the pH of the medium."
(by aatbio)
@@Jehty_ ah, buffer to stabilize pH. Thanks!
I just found this today, and this was my first video. I am so hooked! This is wild! I am amazed. So cool!
Excited for the neuron project part 2/3!
This guy is only one step away from creating life I swear
Mmmm rat borgur 😋
I just love your experiments, I always dreamt of having a lab and trying crazy experiment myself but I don't have the means to get the non-basic apparatus and chemicals! Through your videos, I keep my curiosity calm haha.
I can't thank this channel enough!
I waited so long for your regular videos 😤
Finally ...........!
ah yes, manmade horrors beyond my comprehension
I love how you’re a brilliant scientists AND crafty! 🎉
So glad you're back!
He came back when we needed him most!
Hell yeah! I've been looking forward to more biology videos from you!
Mad scientist does mad scientist stuff, and I'm here for it.
You did it!!! Thanks for the new vid🎉
This is highly weird and is highly interesting! Was not expecting to learn about home made mouse meat this morning lol. Cool video and your explanation of everything you did was good. Just gave you a sub!
Nice, a inquisitive mind with both the skill and patience to get results. I do like where your going with this. The world is a interesting place, keep pealing the layers back, best of luck. I am in on this journey waiting and watching... looking forward to the rest. Cheers!
I love this free high quality education, maybe go in more detail in some topics
Very excited for this to continue
Always amazing! you're on the edge of public free science translated for almost dummies.
glad youre back!
Am so glad I subscribed all those years back
HES STILL ALIVE THANK GOD!!! I have been waiting so long for another video
literally yesterday I was think damn it'd be dope if a new thought emporium video came out lol
I absolutely love your videos, thank you for being awesome!
I am terribly excited to see where this path brings you!
I like at nearing end of the video I was thinking "cool, now you have cells for life, but why?", only to be smacked by an avalanche of jaw-dropping projects this made possible. I love the deliberation in both your work and video scripts.
It's great to see all that I learned at school is being done. I am too poor to have that equipment for myself but oh I would be so glad to have an incubator too
the thinking in the dish part, it made me really question things - A full studio quality photo of that would be so awesome! I'm willing to pay in neurons, no, not my own..
Congrats on 1 million subs
I was thinking about this channel a few days ago but couldn't remember the name and wasn't sure if I could get the right set of keywords together to actually find it again. I'm glad youtube actually recommended it to me so I could watch. And this will be why I'm turning on notifications, too, so I don't have to rely on that to work.
It's so cool to see you work on stuff that my lab does.
I come to your Channel frequently thinking that you might have made a Part two of Neuron video. And I have been doing it for two years!
Even though the other stuff you do is amazing in itself, that Neuron video was the one that introduced me on your channel.
I watched some of your videos with my Elder sister who is a Microbiologist. I am a doctor so I never had the chance to work in Biotech. She was the one who taught me a lot of things about biotech and I also read her books.
I hope your Meat-Robot works next time. ;)
I have probably never used the word fantastic correctly until now. Bravo!
I love the name “meatcubator”! I work in tissue culture. It’s one of my favorite parts of my work. I enjoy taking care of cells and watching them grow up and become more confluent then passage them.
Thank you so much for this awesome content
Great video! Despite an interest I'm not educated in the sciences at all; but I love this channel and watching your technique, knowledge and skills progress. Thanks to people like you we have modern medicine.
I'm also wondering, everything you do in your videos is so perfect; how does the process of making such a video look like? How much do you plan? Did you ever have to start from scratch because something didn't work out? (Mostly asking about chemistry/biochemistry processes, rather than the building equipment part)
Would love to see your process and the hurdles you have overcome.
Finally! A youtube channel that does crazy science
I do not think you understand how extremely good your content is, it's surprising how a person could accomplish such a feats you do on a regular basis.
Been waiting for close to a year to see what you'd get up to. I'm glad that I'm patient to a fault sometimes. It pays.
good to see you back
we do a small amount of ambiguous moral desicions
Oh boy! A new video from the thought emporium, Christmas came early this 2023
this video is 2 hours ago, too bad i dont have the benifit of being late and watching thw whole series. this is basically part 1 to me lol but i subscribed!