He is much more than Nilered. Nilered's experiments are something chemists have been doing for decades. This man over here is bringing cutting edge investigation to his own lab and youtube! Pretty insane.
The fact that the end goal of this series is "create a novel organism like Frankenstein without the lightning" is simultaneously concerning and amazing.
"When humans play gods, it ends in total dismay and destruction, and so we are playing gods now." Can't remember who said that, but i totally agree with that quote.
@@Cyanfox3006 nah, if it's fun it was worth it. In any case, the high adaptability of the human species is rooted in our inclination to play god, as we can adapt the environment to serve us.
The creature from the original telling of Frankenstein wasn’t brought to life by lightning, the lightning thing came from how Victor was inspired to start learning alchemy because he saw a tree get obliterated by lightning (sorry, I’m not intentionally being a book snob, that’s just one of my favorite useless facts)
As a former biomedical engineer who studied tissue engineering and biomaterials, I really like how you simplified the communication surrounding what each of the compounds you were using were for. HBSS, DMEM, FBS... It's amazing what academics take for granted with knowledge of what they think are "basic" things that are quite literally so fundamentally important.
I love how this guy actually takes the time to explain everything hes doing so that people who dont know the technical jargon can actually follow along
Precisely my thoughts. It's hard enough to know all this science, maintain a lab and work in it. The fact that they manage to film everything and explain all the processes with transparence (pun intended) is outstanding!!
So we noticed something very similar when we 3D printed scaffolds from plant based materials and seeded them with fibroblasts....is that the cells were most aligned and viable around the edges and ridges. One of the reasons this could be is that ECM mechanical properties play a crucial role in determining cell orientation and so mechanical properties on the ridges are highest hence fibroblasts will more likely align and grow there most. Interesting work in this video...Our lab works on tissue engineering using 3D printing and I am open to connect
@@lambda653well the thing is that all of science is useless out of context I mean right now it may seem that this only fills useless textbooks but maybe one day we'll find a way to revolutionize travel or find alien civilizations
@@lambda653 understanding how dark matter and energy works could in the far future lead to HUGE innovations in travel, energy generation and stuff we can't even imagine yet
@NexusLore Probably not though. All of those potential discoveries fall under black swan events, which means something fundamental about our understanding of physics completely changes. That's definitely not impossible, but considering how accurate our current theories are, and just how many resources we've already put into to find every possible potential application of every single physics theory, at this point we're pretty sure that if there is a use, it will be so advanced and complicated that we'll never see it come into fruition within the next century. This is not the case with many other fields of science like molecular biology or neuroscience. We're pretty certain that there are world changing advancements waiting to be made in those fields within the next 100 years. Like curing blindness or permanent paralysis. Obviously, there could still be some hookup about human biology that would completely stall our progress in curing blindness, but the difference is that our current scientific knowledge of physics suggests that any practical use for dark matter is completely useless and unfeasible, while our current scientific understanding of biology suggests that it is completely physically feasible to repair or replace eyeballs.
I work in a lab. My work is mostly computational but I've done plenty of cell culture work as well. The fact that youre able to afford this is very impressive.
This guy is single handedly causing me to want to do biology and chemistry, I think that this kind of learning is what's missing from my high-school experience tbh
Several years ago i found this channel during my freshman year, let's just say i was mesmerized by you and the field of bioengineering. And now i have been officially accepted to the one and only Bioengineering program in my country at the best university in my country. You and this channel is very inspiring to me and kept me going on studying my hardest to do stuff like these. Keep doing what you do man, this channel is such a blessing to me.
I hope the rest of your viewers understand how lucky they are that even though you could have ANY career you choose, you’re doing it here for us for free. Thank you, from Nova Scotia, Canada.
You're unnervingly close to making the mythology of homunculus a reality, which is in turn a great first step to making mad science Pokemon. Keep going, I wanna see meat monsters because I have problems and meat monsters are the solution.
Im a young transplant recipient and will need another one in around 20 years or so and this would be a game changer for me, not having to take anti rejection medication would be life changing.
They remainder is pretty much nutritionally useless to humans though. Well it would work as a fibre supplement as cellulose is an indigestible sugar but everything of nutritional value is gone.
I swear we'll see the first human engineered living being not because off super advanced breakthrough but because a dude on RUclips wanted to make a Meat Based Vegetable Fish Also please do more content for the cooking channel
What seems real cool about this, is that, when both the technology of 3d printing gets advanced enough, we could literally print scaffolding and then inject cells into said scaffolding and make our own transplants.
@@growlie2676 We are working on something different for that. (Selective cloning.) Meaning we only grow the parts of the animal we want to eat and it was never a thinking creature. What they are talking about is more for organ repairs.
I'm certain that this -dark magic- tissue engineering series will be my favorite one on youtube for years to come Edit: finding out a few days later that there's a whole strike through workshop going down here
When one of my professors was making the rounds with his spinach leaf heart (Glenn Gaudette, he's now at Boston U but this was when he was at WPI), i started going through different leaf types in my head and what leaves might be best for them as a thought exercise. Incidentally, i think Sage would be useful for building replacement skin, and strong, unidirectionally fibrous leaves (a palm frond, just as an example) may be good for cardiac tissue. Neat stuff!
Let me answer on that, since I am currently working on it. Gaudette did not take in consideration the need for redundancy of the venation pattern in order to achieve functional vascularization. Also, they did not look into the inner structure of the vessels, which makes re-endothelialization unlikely for spinach leaves. Serkan Dikici briefly noted this limitation in a paper in 2019. However, there are plants that are better suited (e.g. lemons, who are naturally redundant). If you open a botanical atlas, you will find plenty of good candidates (mostly from Indonesia)!
@@diegoolivares1081 THAT WAS ONE OF MY FAVORITE ONES, I COULDN'T REMEMBER IT WHEN I WAS Typing THE COMMENT! Closest i could think is a palm frond 🤣🤣🤣. Bananas would be excellent because they're an extremely wasteful crop. The plant the berries grow from lasts only one season. That's a healthy supply of banana leaves right there (save those used for culinary purposes)
@@gmen412 i was wondering about how to deal with the open circulatory system of plants. I wondered if you could perhaps refresh the edges and bond them together somehow if you might get functional capillaries at least (I don't even wanna think about venous valves tbh). My expertise is bionics, however, not tissue engineering. I only have a working knowledge of the processes and concepts, but very little beyond that.
@@mervynlarrier9424 i don't really know anything about this topic, but my uneducated guest is here anyway. Maybe it would be effective scraping one of the sides and then connect it to the expose tissue? So the body could start connecting the blood vessels by itself. I don't know if the skin graft would rott thou
Hi there, great video! I am from the group that originally decellularized spinach leaves a few years ago. Most of my colleagues have moved to lab-grown meat and cell agriculture, but I am still focused on biomedical applications. I am sure you would love my current project, let's talk about it in private! PS: this month I will finally get to meet Dr. Pelling, the guy that carved ears out of apples!
Hi, are you referring to the crossing kingdoms paper (Gershlak et al. 2016)? This was a main paper for a research project we did in my Master's Course and we tried the technique on apples. Nice work.
@@lukasduday8855 Yes, but I joined in 2020 and completely took over the biomedical applications when the other members moved to cellular agriculture. Now we have a funded grant for a skin graft and I am collecting preliminary data for small diameter vascular grafts.
I don't know if you're serious or not. It's hard to tell these days. If you are, how about doing the human race a favor. Find a place as far away from everyone else as possible, and just stay there. None of this kind of thing is going to end well. It's gone too far already.
I feel like you could make a lot of art out of this. Especially with the bone cells. You could turn so many things into a pristine, hard, white 'sculpt.'
The fact you own occult philosophical texts and showcased them in this really makes me believe you embrace how close to dark magic this is becoming atleast aesthetically and I really respect that
@@avokka Science is looking real hard at things and (usually metaphorically) trowing rocks at them to see what happens. By definition "meddling with the unknown". The thing most wrong with your comment is the "fully understood" part. White magic is engineering.
Let's follow the progression, Meat grape, meat leaf and then what's next meat vine? I can't wait till you solve meat roots in episode 4 and move on to the meat vineyard in episode 5
There's also the branch of lab-grown meat as food, i.e. steak that tastes, feels and essentially is real, normal meat but without having to harm animals And just considering that aspect from a climate-perspective seems pretty cool too
You know, I had a grand idea of making an entire fantasy world filled with "animals" that are really just some kind of meat/plant combination (to flesh out my story's world), and this really helps me with my research of making it. This is all so very interesting, gosh darn.
A mesh basket would help with moving delicate items between solutions. If it hangs with space below you can even use a slow magnetic stirer underneath.
13:44 I can't even begin to try and comprehend how costly this joke ended up with, but it did make me chuckle. Here's hoping for more wonderful projects!
this channel is so cool, so glad I discovered it! Also I'm currently a bio major and I feel like the base level of biology knowledge you need for this stuff is literally insane. They do a great job of simplifying it but to even read the research papers to find out how to do this is incredible, props to y'all.
I got into biomedical engineering with the interest of a cellular respiration driven prosthetic. This is actually an interesting step in that direction
I'm a vegan and this video is awesome! Very cool to basically be able to frankenstine-together some completely custom living thing from basically a living cell salad bar. I didn't know this was so easy (by that I mean, it's not prohibitively difficult and expensive). Cool chemistry insight too!
@@bilalbaig8586 Yes, I’ve also read field reports involving trackers talking about how most animals in the wild die in infinitely more horrible ways than our instant death machines in factories. Idk how that’s relevant to my point about the sustainability of growing meat tho. I know it’s not there yet and almost getting eaten by corporations atm, but I have a better idea that’ll piss more people off but work much better- if we grew genetically augmented, brain dead livestock so when we kill them there’s no suffering we wouldn’t have to worry about the texture or quality of anything.
Awesome! Do the cells (continue to) multiply after they adhere to the plant's structure? I'm left wondering if they will eventually fill up the cellulose structure or if it would be required to add new cells from a culture for this.
I’m curious about that because it depends on the epigenetic instructions of the cells. What they are programmed to build. It may not match with the plant’s structure.
from what i know about cell culture, if they can adhere to the cellulose structure and are not dead, theyll continue to multiply. probably not to the point of filling in the entire leaf because iirc anywhere more than a monolayer of cells will result in nutrients not getting to the cells beneath it (since they do not have blood vessels). i imagine the moment the confluency (percent of adherence surface is covered by cells) reaches a 100 theyll all die and fall off en masse though.
I just discovered this channel last night and have been binging it. He doesn't post super often so I feel lucky that I get to see this less than an hour before the start. This channel is incredible.
He was fairly regular before the year or two of his new lab setup (he did do live streams even more informational) and getting all the infrastructure for the projects organised. So I predict and hope that the posting will increase.
He's also removed quite a few videos which may or may not be on his patreon. It's definitely worth throwing a few dollars his way, even if you can only do it for one month. A single month of patreon is worth tens of thousands of views on youtube.
Similar can be done with skin cells on deceullarized cartilage medium for specific types of skin grafts. Some dont even use the cartilage medium and just let the skin form sheets for the skin graft.
Anf for sure it will cost tens of thousands dollars for a small engineered muscle (organs even more). I can remember I have read ancient Sci-fi literature from Isaac Asimov or Stanislaw Lem describing organ generators like this.
@@ZoonCrypticon It will probably depend on the country and how much the process can be streamlined. If you're in the US its probably gonna be a fortune anyway yeah.
I got a midroll ad for the leaking spinach at 5:20 for leak-free gorilla glue! Also, this inspires me so much. I’m considering doing my senior design project on this topic (I’m a bioengineering major) and these videos really help with explaining. Thanks!
My ad was for Bounty: The Quicker Picker Upper. 😂 (For anyone not familiar, it's a brand of paper towels branded as more absorbant than the competition).
Theoretically, could you take a dense branching fruit or vegetable, like a cabbage, broccoli, or melon, decellularize it, and refill it with neuron cells to create a brain?
@@nobody.of.importance Im not saying it would work well. I just am interested to see how well a bundle of neurons organize themselves in an unfamiliar environment and attempt to form a brain. Insects have very simple (but obviously complex compared to complete scratch) brains that are capable of surprising levels of thought. Surely you could approximate that to some degree.
@@pauldeddens5349 Fair, I'm just not sure you could really call it a "brain" per se. If you're curious, you should look into cerebral organoids, they're pretty close to what you're describing.
Oh, using the bone cells to fill up plant scaffold sounds super cool and artsy. I could easily see that being used as building decor - like those bone chapels in Europe. I wonder, if you could produce this way a cheaper cruelty free versions of the elephant tusk, if the technology progresses enough to produce bigger objects.
Should be pretty easy in theory. Get cells that produce mammal teeth, get it growing with enough materials to build the tusk and set it up into mold to form the shape. It may not have durable outer shell of emal, but producing tusk that is entirely of dentine should be possible, just need to break the mold for each and any contamination might be a issue, while taking long time, but it should be possible.
Every time I watch one of your videos I feel very validated both in my decision to become a scientist when i was 6 and also in ditching physics for biology after I had an internship in particle accelerator lab. Biology and medicine rule!
I would say since it's a 50/50 hybrid (scaffold plus contents) it is at the same also meat made out of a leaf. The meat was what was added, the leaf was already there as a scaffold...
OUR LORD AND SAVIOUR FUNNY GENETICS MAN HATH BLESSED US (notification gang). seriously though, your channel single handedly cultivated my interest in science as a broader subject instead of just the engineering stuff im learning as a part of my degree, and thank you for that.
I have not messed with decellularization but I have with pumping liquids through leaves, and i figured out that it is good to think of it as a circuit. It needs a in, a out. and everything needs to be connected up. The best place to start is to get extremely fresh leaves. It also takes some time to find a good place for exit. But the exit has to be quite small.
Thank you for the play by play with the reagents. I am working on the transparent wood project, but i may use peroxide for the bleaching. I used acetone to dry out the wood, but will probably use dawn power wash for my detergent step, but any degreaser could work i feel. I think patience will be the key. All the variations of the experiment i have seen have done too much cooking and this gentle approach seems very viable. Thanks for the vid. Your CRISPR stuff brought me to your channel.
Hey there! I've been following the channel for years and I love the content. I found inspiration in your meat grape video years ago to base my BSc thesis on it. Currently I'm doing an internship at Mosa Meat, growing muscle tissue for human consumption. Keep it up, your videos may be inspiration for many bright minds (not me)!!
I love how casual and upbeat he is as he explains the steps to creating a frankenstein’s monster-esque horror, then happily suggests that if another project goes well, he could give it a functioning brain made of rat neurons
I love the details that you shared in every step of the process! You and your team make the most informative videos. Please keep them coming and congratulations on what you just accomplished!!
XD evne though all the animals used in the meat industry are solely raised for this task. unless ya getting deer or something and that place has a sessional menue
I just watched the mestcubator video in my watch later. So I went baxk and watched meat berty and head cheeses 1.0 Now I will watch meat leaf and rhen head cheese 2.0 I rarely jump my watch later, and knowing that such great videos are eventually in it... Motivates me to keep watching. And doing a jump, is my Christmas treat this year.
I'm convinced that everything we are, and ever will be, is all a part of this guy's mad science! Kidding aside, weren't we all just substrate grown in our mother's wombs at one point? This is a fascinating channel; so glad that I subscribed!
1:20 but would the organs be functional? Does the heart muscle, for instance, actually have strength that is attained only through repeated resistance movement? Is that part of the challenge and why something like this hasn’t yet been implanted into a human? I have no idea, just asking if anyone does.
I love your channel so much, your doing exactly what I would love to do. Trying to go back to school for cell biology because I wanna get into cell culturing and synthetic biology!
it's always a cool idea considering that there are procedures that wrap the affected area of the skin with fish scale for proliferation. So many things to consider, I just thought it was funny that it comes full circle, from the vegetable fish theory to the skin graft.
Kinda freaky, but really cool project! I just wonder how easy it would be to take this one step further and grow actual organ-like structure; after all, at the moment these are just mesenchymal cells, whereas most tissues also have epithelial layers. I would expect it to be pretty hard to basically get the different cells into the right places with the current seeding method...
Hey 😊 vegetarian here. I was not offended even once on this video. I know for the moment we still need to use some animal components on this process but MY MAN, you are advancing and promoting a technology that will one day free millions of animals each year from suffering. Thank you for the video, amazing as always. So hyped to know the neuron proyect it's being worked on 😄
Vegetarians pay for dairy/eggs which are industries arguably more horrific than the meat industry. If you're not vegan, you're just as bad as a meat eater, you're paying for animal torture either way.
This man is basically the biology version of Nilered and I'm all for it
With less failed attempts 😂 at least from what's being shown in videos
@@MagicalKidImagine he has a "failed attempt" while making a virus and makes the next pandemic lmfao
He is much more than Nilered. Nilered's experiments are something chemists have been doing for decades. This man over here is bringing cutting edge investigation to his own lab and youtube! Pretty insane.
His alcohol aging video had him sharing a bunch of drinks with Nile and Nile just says "They all taste the same".
They're IRL friends so that's probably not an accident that they have similarities
The fact that the end goal of this series is "create a novel organism like Frankenstein without the lightning" is simultaneously concerning and amazing.
man made horrors
"When humans play gods, it ends in total dismay and destruction, and so we are playing gods now." Can't remember who said that, but i totally agree with that quote.
@@Cyanfox3006 nah, if it's fun it was worth it. In any case, the high adaptability of the human species is rooted in our inclination to play god, as we can adapt the environment to serve us.
The creature from the original telling of Frankenstein wasn’t brought to life by lightning, the lightning thing came from how Victor was inspired to start learning alchemy because he saw a tree get obliterated by lightning (sorry, I’m not intentionally being a book snob, that’s just one of my favorite useless facts)
@@Ophelia7438that's actually a cool fact! I didn't knew that
As a former biomedical engineer who studied tissue engineering and biomaterials, I really like how you simplified the communication surrounding what each of the compounds you were using were for. HBSS, DMEM, FBS...
It's amazing what academics take for granted with knowledge of what they think are "basic" things that are quite literally so fundamentally important.
🤓
@@josephdogg1shut the hell up and appreciate the original comment
@@josephdogg1Whats wrong with that? Let them be
@@josephdogg1typical illiterate 10 year old.
@@YouareAlreadyDead700 🤓
Please dont stop this series i want to see veggie fish!
same lol.
Yeah
I want to learn how to make a meat Miku so I can keep it as a pet
@@beccasflyingrainbow7886 the horrors of human imagination
I love how this guy actually takes the time to explain everything hes doing so that people who dont know the technical jargon can actually follow along
Thanks to these *clear* instructions, I was able to make a beating heart!.... I hate it
Precisely my thoughts. It's hard enough to know all this science, maintain a lab and work in it. The fact that they manage to film everything and explain all the processes with transparence (pun intended) is outstanding!!
This is how vita carnis universe began...
he knows that nobody thats actually smart wants to watch him make a meat vegetable fish, but i do 😂
I still don’t understand tho
I still want you to take a meat base, decellularize it, and give it plant based cells. I want to see a plant monster.
so a plant based heart
@jamescheddar4896 Dude fck veganism, we go for photoism :D Straight from the soure itself!
If you want a vegetable I can give you my braindead mother in law
@James Cheddar i don't think you could live off of water and the sun, concerning the efficiency of photosynthesis...
Monster is a subjective term
meat leaf, meat based robot, and vegetable fish are word combinations i never thought i would hear
Meat salad
@@Zamu273Meat salad
imagine a pork mango
Fish IS a vegetable.
-Ron Swanson
You just haven't read enough wh40k lore
So we noticed something very similar when we 3D printed scaffolds from plant based materials and seeded them with fibroblasts....is that the cells were most aligned and viable around the edges and ridges. One of the reasons this could be is that ECM mechanical properties play a crucial role in determining cell orientation and so mechanical properties on the ridges are highest hence fibroblasts will more likely align and grow there most. Interesting work in this video...Our lab works on tissue engineering using 3D printing and I am open to connect
1st
I love how science goes from things like "how does the universe move" to "we're growing a meat leaf"
Tbf, the meat leaf is actually a lot more useful for humanity than trying to understand how dark matter works.
@@lambda653well the thing is that all of science is useless out of context I mean right now it may seem that this only fills useless textbooks but maybe one day we'll find a way to revolutionize travel or find alien civilizations
First learn how this stuff works, then do stuff with it
@@lambda653 understanding how dark matter and energy works could in the far future lead to HUGE innovations in travel, energy generation and stuff we can't even imagine yet
@NexusLore Probably not though. All of those potential discoveries fall under black swan events, which means something fundamental about our understanding of physics completely changes. That's definitely not impossible, but considering how accurate our current theories are, and just how many resources we've already put into to find every possible potential application of every single physics theory, at this point we're pretty sure that if there is a use, it will be so advanced and complicated that we'll never see it come into fruition within the next century. This is not the case with many other fields of science like molecular biology or neuroscience. We're pretty certain that there are world changing advancements waiting to be made in those fields within the next 100 years. Like curing blindness or permanent paralysis. Obviously, there could still be some hookup about human biology that would completely stall our progress in curing blindness, but the difference is that our current scientific knowledge of physics suggests that any practical use for dark matter is completely useless and unfeasible, while our current scientific understanding of biology suggests that it is completely physically feasible to repair or replace eyeballs.
I work in a lab. My work is mostly computational but I've done plenty of cell culture work as well. The fact that youre able to afford this is very impressive.
How expensive do you think it would be to do this process once? Ballpark
@@Ton12extremely. Need for extremely sterile conditions and components like FBS make it very expensive.
Ballpark? If you had no equipment or facility, I'm assuming a minimum investment of at least $100,000 USD@@Ton12
meat leaf
I also work in a lab, and I wondering if he has a lab or something, because how would he have all this 😭
I heard the term "meat-based robot" for the very first time today, and the things that immediately came to mind are horrific
I mean, arent we not meat based robots?
@@discountpotato5680 we are not. we are living organisms
@@breadcraft3605 What is an organism?
@@jimijenkins2548 A meat based robot
@@BBBBBBBBBBBBBBBB2 But enough chatter. Have at ye!
“Hey how’s the experiment going?”
“my leaf exploded.”
Minecraft creeper
My leaves keep springin leaks
Danggett
This guy is single handedly causing me to want to do biology and chemistry, I think that this kind of learning is what's missing from my high-school experience tbh
School is 1000x better in college than in high-school (depending on the school) small interests turn into fields of research and career
meat leaf.
@@notchillstorm please dont hurt me
I mean... who doesn't want to make meat leaves (aside from the vegans)
@@Elledrasorcery
Several years ago i found this channel during my freshman year, let's just say i was mesmerized by you and the field of bioengineering. And now i have been officially accepted to the one and only Bioengineering program in my country at the best university in my country. You and this channel is very inspiring to me and kept me going on studying my hardest to do stuff like these. Keep doing what you do man, this channel is such a blessing to me.
I’m happy for you congrats
I hope the rest of your viewers understand how lucky they are that even though you could have ANY career you choose, you’re doing it here for us for free. Thank you, from Nova Scotia, Canada.
Another thanks from New Brunswick :)
Leaf meat watching the development of their antithesis.... Meat leaf.
10 years ago: "Evil Chinese scientists are making chimeras in secret labs!"
Now: Let's put rat-cells in leaves on youtube!
So true
You're unnervingly close to making the mythology of homunculus a reality, which is in turn a great first step to making mad science Pokemon. Keep going, I wanna see meat monsters because I have problems and meat monsters are the solution.
The picatrix would be a fun read for you
Yeah, there is a strong Herbert West vibe to these projects.
no he isn't. I understand you want to feel like you are included and knowledgeable but you aren't. Stop spreading nonsense.
@@thomgizziz get a sense of humor
Let’s hope bro doesn’t find any mews. . .
Finally, we've had vegetarian versions of meat dishes for years now. It's about time we got a meat version of a vegetarian thing lol
Meatatarian Plant dinners
A small population of humans are transgender. Then there have been these jokes about transveganism. In that vein, would these be called.. transplants?
I think popeyes once made a carrot shaped sausage, they called it the "marrot"
What kindof of dish is “leaf”
How does a meat leaf salad sound?
Im a young transplant recipient and will need another one in around 20 years or so and this would be a game changer for me, not having to take anti rejection medication would be life changing.
I don't really see how this will pass the final hurdle of human tests
@@ayoCCyeah there's too many close minded folks who will be too afraid of progress to try to save lives
Shots fired at Nile Red, My man's a Savage.
5:16 Finally, a realistic depiction of lab work:D
Great job as always!
literally i feel that in my soul
so true, as an organic chem major that really does encapsulate the lab experience
I thought he was just channeling AvE there for a bit.
Me at night :
sussiest part
The decellularization process really puts in a new meaning to cleaning your veggies before you eat em
They remainder is pretty much nutritionally useless to humans though. Well it would work as a fibre supplement as cellulose is an indigestible sugar but everything of nutritional value is gone.
hah it looks like techno blades remains lmao
@@phlegmony tf
@@phlegmony What a monster.
I swear we'll see the first human engineered living being not because off super advanced breakthrough but because a dude on RUclips wanted to make a Meat Based Vegetable Fish
Also please do more content for the cooking channel
Gotta define "living being" first though. :3
Meat Based Vegetable Fish with a brain on a chip interfacing with a computer........ Remote controlled meat based vegetable fish.
@@Dogo.R a being that feeds, grows and breathes. We can set aside the "procreation" part, because then we must consider all barren people are dead.
This *is* the cooking channel.
@@Dogo.R featherless biped
Surprise sonny's edge mention got me remembering how much i loooove that short!!!
What seems real cool about this, is that, when both the technology of 3d printing gets advanced enough, we could literally print scaffolding and then inject cells into said scaffolding and make our own transplants.
there’s literally the goal dude, catch up
Would this mean that we could never run out of meat to use or eat?
@@growlie2676 We are working on something different for that. (Selective cloning.) Meaning we only grow the parts of the animal we want to eat and it was never a thinking creature.
What they are talking about is more for organ repairs.
I was thinking the same thing.
@@growlie2676such a thing already exists. it's called farming
I'm certain that this -dark magic- tissue engineering series will be my favorite one on youtube for years to come
Edit: finding out a few days later that there's a whole strike through workshop going down here
How do you do the crossed out text?
@@2iLikeCats2 dark magic?
-text-
@@2iLikeCats2basically you do this
- text -
(Without adding any space between the dash and the text)
Ohhh -text- cool
When one of my professors was making the rounds with his spinach leaf heart (Glenn Gaudette, he's now at Boston U but this was when he was at WPI), i started going through different leaf types in my head and what leaves might be best for them as a thought exercise. Incidentally, i think Sage would be useful for building replacement skin, and strong, unidirectionally fibrous leaves (a palm frond, just as an example) may be good for cardiac tissue. Neat stuff!
Maybe banana leaf could be useful for that
Let me answer on that, since I am currently working on it.
Gaudette did not take in consideration the need for redundancy of the venation pattern in order to achieve functional vascularization. Also, they did not look into the inner structure of the vessels, which makes re-endothelialization unlikely for spinach leaves. Serkan Dikici briefly noted this limitation in a paper in 2019.
However, there are plants that are better suited (e.g. lemons, who are naturally redundant). If you open a botanical atlas, you will find plenty of good candidates (mostly from Indonesia)!
@@diegoolivares1081 THAT WAS ONE OF MY FAVORITE ONES, I COULDN'T REMEMBER IT WHEN I WAS Typing THE COMMENT! Closest i could think is a palm frond 🤣🤣🤣. Bananas would be excellent because they're an extremely wasteful crop. The plant the berries grow from lasts only one season. That's a healthy supply of banana leaves right there (save those used for culinary purposes)
@@gmen412 i was wondering about how to deal with the open circulatory system of plants. I wondered if you could perhaps refresh the edges and bond them together somehow if you might get functional capillaries at least (I don't even wanna think about venous valves tbh). My expertise is bionics, however, not tissue engineering. I only have a working knowledge of the processes and concepts, but very little beyond that.
@@mervynlarrier9424 i don't really know anything about this topic, but my uneducated guest is here anyway. Maybe it would be effective scraping one of the sides and then connect it to the expose tissue? So the body could start connecting the blood vessels by itself. I don't know if the skin graft would rott thou
"...and evidenty it is much easier to make leaves go clear than wood"
Didn't expect the thought emporium to throw a shade at nilered but damm
Hi there, great video!
I am from the group that originally decellularized spinach leaves a few years ago. Most of my colleagues have moved to lab-grown meat and cell agriculture, but I am still focused on biomedical applications.
I am sure you would love my current project, let's talk about it in private!
PS: this month I will finally get to meet Dr. Pelling, the guy that carved ears out of apples!
He may have a business email on his RUclips page if this comment doesn’t get his attention.
Hi, are you referring to the crossing kingdoms paper (Gershlak et al. 2016)? This was a main paper for a research project we did in my Master's Course and we tried the technique on apples. Nice work.
@@lukasduday8855 Yes, but I joined in 2020 and completely took over the biomedical applications when the other members moved to cellular agriculture. Now we have a funded grant for a skin graft and I am collecting preliminary data for small diameter vascular grafts.
Poor apple
I don't know if you're serious or not. It's hard to tell these days. If you are, how about doing the human race a favor. Find a place as far away from everyone else as possible, and just stay there. None of this kind of thing is going to end well. It's gone too far already.
I feel like you could make a lot of art out of this. Especially with the bone cells. You could turn so many things into a pristine, hard, white 'sculpt.'
Bone sculptures are already a thing. Chrizelephantine and carved bone been known since ancient greece.
@@secretname2670Yeah but it's Cooler when you know it's bioengineered y'know
@@bronze1557 thats a oneshot idea, it'll get boring after the limelight of novelity goes out.
@@secretname2670 All art is temporary anyways
@@secretname2670you dont have to be a buzzkill lol
The fact you own occult philosophical texts and showcased them in this really makes me believe you embrace how close to dark magic this is becoming atleast aesthetically and I really respect that
Science in my opinion is just fully understood dark magic, it's so fucking bizarre sometimes
@@avokka Science is looking real hard at things and (usually metaphorically) trowing rocks at them to see what happens. By definition "meddling with the unknown". The thing most wrong with your comment is the "fully understood" part.
White magic is engineering.
@@marcosalmeida3947so dark magic possible kinda bad idea considering military use may be a thing
WHERE WAS THIS WHAT
@avokka There's definitely a validity to Fire Emblem 3H rebranding all its Dark magic to "Reason" magic!
i’ve never been so bored and entertained at the same time
Let's follow the progression, Meat grape, meat leaf and then what's next meat vine? I can't wait till you solve meat roots in episode 4 and move on to the meat vineyard in episode 5
Meat Tree
Delicious meaty Meat Tea
mmm, meat wine
When the plants give you a high five on the way into the winyard but they have no mouth so they cannot scream. XD
The ultimate goal is resurrecting meatloaf...
He would do anything for... Science...
I'm so glad you explained that these are a stepping stone to tailored organ transplants. That's the direction this research needs to go.
There's also the branch of lab-grown meat as food, i.e. steak that tastes, feels and essentially is real, normal meat but without having to harm animals
And just considering that aspect from a climate-perspective seems pretty cool too
No.
We need vegetable fish
I am both terrified and fascinated. I wonder how much further bio engineering will progress in the next 27 years.
flying fish 💀😭
Godzilla vs King Kong irl. Or the flood from Halo.
Vita carnis irl?
@@ismeldagzz5056 I hope not
@@TheUlquiorraCifer More like, ads made out of meat. All everything comes down to ads
You know, I had a grand idea of making an entire fantasy world filled with "animals" that are really just some kind of meat/plant combination (to flesh out my story's world), and this really helps me with my research of making it. This is all so very interesting, gosh darn.
I salute your ability to show complex topics in a way that is simple enough to entertain yer average viewer.
Scientifically accurate transmutation! Loved it
Scientifically accurate transmutation is just Nuclear Fission/Fusion.
8:17 hehe that nile red reference
7:52 my favourite drink! thank you for using it
Hmm tasty right!
@@Leda_Bernardinofr
A mesh basket would help with moving delicate items between solutions. If it hangs with space below you can even use a slow magnetic stirer underneath.
would the magnetic stirer crumple the leaf? Great suggestion tho
13:44 I can't even begin to try and comprehend how costly this joke ended up with, but it did make me chuckle. Here's hoping for more wonderful projects!
The compilation of liquid spraying everywhere and him swearing profusely absolutely made my day
Loved the protocol dude. I worked in a hMSC cell culture lab and I am envious your home lab!
this channel is so cool, so glad I discovered it! Also I'm currently a bio major and I feel like the base level of biology knowledge you need for this stuff is literally insane. They do a great job of simplifying it but to even read the research papers to find out how to do this is incredible, props to y'all.
I got into biomedical engineering with the interest of a cellular respiration driven prosthetic. This is actually an interesting step in that direction
I loved the concept of 100% meat based spinach salad selling right in front a 100% plant based meat shop 😂😂😂😂😂
I want to see that SO bad!!
This is actually so dope and should be fully funded like right away bro and hopefully is
This is so mind blowing, ten minutes ago I didn't even know that this was even within the possible. Now I'm obsessed
I'm a vegan and this video is awesome! Very cool to basically be able to frankenstine-together some completely custom living thing from basically a living cell salad bar.
I didn't know this was so easy (by that I mean, it's not prohibitively difficult and expensive).
Cool chemistry insight too!
Subscribed, this guy is amazing. Love the comedic touch
And ayy, if we could sustainably grow our own meat, farming would be completely unnecessary. So this is all good for other animals as well.
@@suruxstrawde8322 Have you ever seen a lion eat a deer?
@bilalbaig8586 what does this mean? 🤨
@@bilalbaig8586
Yes, I’ve also read field reports involving trackers talking about how most animals in the wild die in infinitely more horrible ways than our instant death machines in factories.
Idk how that’s relevant to my point about the sustainability of growing meat tho. I know it’s not there yet and almost getting eaten by corporations atm, but I have a better idea that’ll piss more people off but work much better- if we grew genetically augmented, brain dead livestock so when we kill them there’s no suffering we wouldn’t have to worry about the texture or quality of anything.
As a bioengineer learning about this exact thing at a university that specializes in biomed cardiology- this is a very good video
This guy is my favorite mad scientist
*"Oh sh!t, Sh!t, F@CK!"* haha @ 5:24
Best montage ever! 🤬-Sh!t
and then the captions are just
[ __ ] [ __ ] etc
Awesome! Do the cells (continue to) multiply after they adhere to the plant's structure? I'm left wondering if they will eventually fill up the cellulose structure or if it would be required to add new cells from a culture for this.
I’m curious about that because it depends on the epigenetic instructions of the cells. What they are programmed to build. It may not match with the plant’s structure.
Yes they are
from what i know about cell culture, if they can adhere to the cellulose structure and are not dead, theyll continue to multiply. probably not to the point of filling in the entire leaf because iirc anywhere more than a monolayer of cells will result in nutrients not getting to the cells beneath it (since they do not have blood vessels). i imagine the moment the confluency (percent of adherence surface is covered by cells) reaches a 100 theyll all die and fall off en masse though.
I just discovered this channel last night and have been binging it. He doesn't post super often so I feel lucky that I get to see this less than an hour before the start. This channel is incredible.
He was fairly regular before the year or two of his new lab setup (he did do live streams even more informational) and getting all the infrastructure for the projects organised. So I predict and hope that the posting will increase.
He's also removed quite a few videos which may or may not be on his patreon. It's definitely worth throwing a few dollars his way, even if you can only do it for one month. A single month of patreon is worth tens of thousands of views on youtube.
Similar can be done with skin cells on deceullarized cartilage medium for specific types of skin grafts. Some dont even use the cartilage medium and just let the skin form sheets for the skin graft.
5:31 😅
Watching those leaves “explode” followed by your repeated F-bombs has started a giggle-fest that just won’t stop
I really hope this becomes used in medicine soon, it's such a game changer.
Anf for sure it will cost tens of thousands dollars for a small engineered muscle (organs even more). I can remember I have read ancient Sci-fi literature from Isaac Asimov or Stanislaw Lem describing organ generators like this.
@@ZoonCrypticon It will probably depend on the country and how much the process can be streamlined. If you're in the US its probably gonna be a fortune anyway yeah.
I got a midroll ad for the leaking spinach at 5:20 for leak-free gorilla glue!
Also, this inspires me so much. I’m considering doing my senior design project on this topic (I’m a bioengineering major) and these videos really help with explaining. Thanks!
My ad was for Bounty: The Quicker Picker Upper. 😂
(For anyone not familiar, it's a brand of paper towels branded as more absorbant than the competition).
oof midroll algorithms are scary
the clear, decellularized leaves look so cool, like jellyfish
Theoretically, could you take a dense branching fruit or vegetable, like a cabbage, broccoli, or melon, decellularize it, and refill it with neuron cells to create a brain?
Sadly no, brains are highly organized structures that need various support systems to help guide their axons to the intended target.
@@nobody.of.importance And things wouldn't be very well connected.
@@nobody.of.importance Im not saying it would work well. I just am interested to see how well a bundle of neurons organize themselves in an unfamiliar environment and attempt to form a brain.
Insects have very simple (but obviously complex compared to complete scratch) brains that are capable of surprising levels of thought. Surely you could approximate that to some degree.
@@pauldeddens5349 Fair, I'm just not sure you could really call it a "brain" per se. If you're curious, you should look into cerebral organoids, they're pretty close to what you're describing.
@@nobody.of.importance kind of like assembling a computer but not installing any operating system?
Oh, using the bone cells to fill up plant scaffold sounds super cool and artsy. I could easily see that being used as building decor - like those bone chapels in Europe.
I wonder, if you could produce this way a cheaper cruelty free versions of the elephant tusk, if the technology progresses enough to produce bigger objects.
Should be pretty easy in theory. Get cells that produce mammal teeth, get it growing with enough materials to build the tusk and set it up into mold to form the shape. It may not have durable outer shell of emal, but producing tusk that is entirely of dentine should be possible, just need to break the mold for each and any contamination might be a issue, while taking long time, but it should be possible.
Finally, using bone blocks in Minecraft is now known as *real-life*
Decellurize a banana, colonize it with elephant tooth cells, and you should have a nice small scale experiment
Hehehe bone furniture Is getting clóser to reality for the average person
Every time I watch one of your videos I feel very validated both in my decision to become a scientist when i was 6 and also in ditching physics for biology after I had an internship in particle accelerator lab. Biology and medicine rule!
I think biologicy is just the best choice tbh
the NileRed of Biology. keep making incredible content Justin!
You've made a leaf out of meat, but when are you gonna make meat out of a leaf?
Green broccoli rat hearts.
@@MauroTammI prefer the spinach monkey brains
Strike that....reverse it.
I would say since it's a 50/50 hybrid (scaffold plus contents) it is at the same also meat made out of a leaf. The meat was what was added, the leaf was already there as a scaffold...
You can do that yourself by just… eating a leaf
1:17 wait, THEYVE ALREADY DONE IT SUCCESSFULLY?! That’s actually so incredible. Wow!!
2:20 this man is bringing cloudy with a chance of meatballs to life
Great movie btw👍🏻👍🏻👍🏻
This is the first video I have ever watched from you .
OUR LORD AND SAVIOUR FUNNY GENETICS MAN HATH BLESSED US (notification gang). seriously though, your channel single handedly cultivated my interest in science as a broader subject instead of just the engineering stuff im learning as a part of my degree, and thank you for that.
5:10 would it even be science without a lot of behind-the-scenes swearing?
Watching you reconstructing the whole method was an extraordinary experience! bioengineering is becoming closer to a mundane level
Imagine being jumpscared by shattered chica and start flapping your leaf around while live streaming
yea imagine, never happened to me
I like how humans went from making hammers out of sticks to turning leaves into meat lol
I have not messed with decellularization but I have with pumping liquids through leaves, and i figured out that it is good to think of it as a circuit. It needs a in, a out. and everything needs to be connected up. The best place to start is to get extremely fresh leaves. It also takes some time to find a good place for exit. But the exit has to be quite small.
Thank you for the play by play with the reagents. I am working on the transparent wood project, but i may use peroxide for the bleaching. I used acetone to dry out the wood, but will probably use dawn power wash for my detergent step, but any degreaser could work i feel. I think patience will be the key. All the variations of the experiment i have seen have done too much cooking and this gentle approach seems very viable. Thanks for the vid. Your CRISPR stuff brought me to your channel.
Can’t wait to see the next part!😊
Wow I remember the meat berry one and how not-so-well it went.
Amazing improvement.
as a fan of living machines made out of flesh I feel this series will be one of my favourites
Hey there! I've been following the channel for years and I love the content. I found inspiration in your meat grape video years ago to base my BSc thesis on it. Currently I'm doing an internship at Mosa Meat, growing muscle tissue for human consumption. Keep it up, your videos may be inspiration for many bright minds (not me)!!
I love how casual and upbeat he is as he explains the steps to creating a frankenstein’s monster-esque horror, then happily suggests that if another project goes well, he could give it a functioning brain made of rat neurons
I love the details that you shared in every step of the process! You and your team make the most informative videos. Please keep them coming and congratulations on what you just accomplished!!
0:46 ''Because when idiots like myself-" If he's an idiot WHAT DOES THAT MAKE ME?!?!?!?!
-5 IQ
Rat cells probably, Lol
Nice shout out to Nile Red!! you guys are the best!
Meat robots?i like the sound of that
as a vegetarian who hasn’t had a cheeseburger in about a decade… please god let this technology advance as fast as possible
XD evne though all the animals used in the meat industry are solely raised for this task. unless ya getting deer or something and that place has a sessional menue
Did you hear the part about the baby cow juice?
Wait until you find out what fetal bovine serum is made from...
@@Kruzhh Oh god don't tell us you're a pro-lifer
@@Kruzhh If it's an ethical choice against animal farming then there isn't any problem with lab grown meats from fetal stem-cells
OH YEAH! so excited to see an update on this project :)
Excellent experiment! I love to think that the future of technology is growing our own organisms from scratch.
I just watched the mestcubator video in my watch later. So I went baxk and watched meat berty and head cheeses 1.0
Now I will watch meat leaf and rhen head cheese 2.0 I rarely jump my watch later, and knowing that such great videos are eventually in it... Motivates me to keep watching. And doing a jump, is my Christmas treat this year.
I'm convinced that everything we are, and ever will be, is all a part of this guy's mad science! Kidding aside, weren't we all just substrate grown in our mother's wombs at one point? This is a fascinating channel; so glad that I subscribed!
Now I need to see NileRed try making clear leaves
@@LostSoulchild89 We need more!
I just love the idea of "fake" vegetables and fruits made from meat just to counter all the fake meat products.
1:20 but would the organs be functional? Does the heart muscle, for instance, actually have strength that is attained only through repeated resistance movement? Is that part of the challenge and why something like this hasn’t yet been implanted into a human? I have no idea, just asking if anyone does.
I love your channel so much, your doing exactly what I would love to do. Trying to go back to school for cell biology because I wanna get into cell culturing and synthetic biology!
Science is wonderfully complicated and confusing, but beautiful when you get the basic idea of what happening.
imagine using giant leaves to create giant skin grafts
this is talked about in ssp circles
it's always a cool idea considering that there are procedures that wrap the affected area of the skin with fish scale for proliferation. So many things to consider, I just thought it was funny that it comes full circle, from the vegetable fish theory to the skin graft.
You’re an absolute madman… basically, an evil genius! A freaking love this.
1:48 But we already have been making meat robots for millenia. We just have a very limited set of models. Like "human" or "dog"
Kinda freaky, but really cool project!
I just wonder how easy it would be to take this one step further and grow actual organ-like structure; after all, at the moment these are just mesenchymal cells, whereas most tissues also have epithelial layers. I would expect it to be pretty hard to basically get the different cells into the right places with the current seeding method...
I think that's actually something currently being studied for medical use! as well as biological 3d printing lol
Hey 😊 vegetarian here. I was not offended even once on this video. I know for the moment we still need to use some animal components on this process but MY MAN, you are advancing and promoting a technology that will one day free millions of animals each year from suffering. Thank you for the video, amazing as always. So hyped to know the neuron proyect it's being worked on 😄
Vegetarians pay for dairy/eggs which are industries arguably more horrific than the meat industry. If you're not vegan, you're just as bad as a meat eater, you're paying for animal torture either way.
Nobody
@@vinbin423 I'll delete if you think it's a harmful opinion, sorry for discomforting you :(
@@KirssarGamessorry to tell you, but everyone has opinions. people will bash your opinions, and you just need to resist the bashing of your opinion.
@@The_Moth1 trying to do it more recently, thanks 😁
This channel is going to make a full on servitor eventually. I'm calling it now.
A computer with robot parts controlling an organic body.
Basically meat mindflayer