2D Gel Electrophoresis (2) Protein Quantitation

( www.abnova.com ) - Extracted proteins can be resuspended in rehydration buffer and quantified by using Bradford assay. This AbVideo introduces the detailed procedure of measuring the protein concentration in a solution. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight). More videos at Abnova www.abnova.com