Really insightful, thanks a lot. Please how can i determine purity of a protein after running SDS-PAGE and staining with coomassie blue? Please immediate response will be very much appreciated
would you mind to help me with a bit problem I have? The thing is that I do not understand how can I get the amplicon size exactly in each PCR band. Can you help me on this? Thank you!!
I was looking for more info on quantitation. I am less interested in migration or size determination... that's what a marker is for and can easily be determined by eye most of the time. The reason I use this type of software is for band intensity comparison as a means to quantitate concentration. This is a common practice using ImageJ. I was hoping to find the equivalent method for this software in this video. Do you have other videos that explain quantitation, including dilution factors to accommodate for the volumes loaded in the marker?
Hi Casey. Its been many years since I posted this tutorial, and I haven't needed to work with it lately, so I may not be the best person to ask about this. What you are doing in terms of reverse-engineering the concentration seems interesting, but outside of the intensity listed on the y-axes I don't know of a means to do this with this particular software suite off the top of my head. That said, I would definitely recommend reaching out to Istvan Lazar, who is the main developer and who has continued adding enhancements to the software suite over the years. You should be able to find the contact information for him as well as others on the team at the link I provided in the description.
I have some question about Gel Analyzer. So when I switch to MW calibration mode and I write the values for the molecular weights it seems it can't save what I write. I hovered over the dot, wrote base pair and pressed enter but it did't take. What could be the problem? Thank you for your help. :)
It has been a while since I made this video (my apologies to everyone whose questions I've missed over the years), but from what I can remember this sounds very similar to what happened to me at 6:36 in the video. Pressing "Enter" with NumLock enabled on my keypad worked from what I can recall. If that's not the case for you, then I would try troubleshooting different file formats (.jpg, .png, .bmp) to see if that makes any difference. As a last resort, maybe uninstalling and reinstalling GelAnalyzer may correct your issue.
It's been about 5 years since I made this video, but I do remember that the MW values are typically standard and known ahead of time. I was reading off of a reference paper from the lab.
Not really explained. You speak words like "values" and dunno, - but what values? What? Why? When? Or Why do you manually select bands? What is the point using this kind of software when you are detecting your bands manually? Ah.
This video was made over 7 years ago. I haven't looked recently, but I wouldn't be surprised if the more recent versions of this program (or others like it) have improved a lot and require less manual work. Nowadays, it is even faily trivial to train a basic image recognition model using basic libraries and frameworks from Python.
This is a really excellent tutorial and it was very helpful for my project, so thanks a bunch!
It really helps a lot! Thank for this tutorial! Were from phil!
Awesome. This helps me a lot. Thanks!
Thank you very much for this video guidance
Good tutorial, thanks mate!
Thank you this is so really helpful for me. GBU
thank you , proved very helpful for me
Really insightful, thanks a lot. Please how can i determine purity of a protein after running SDS-PAGE and staining with coomassie blue? Please immediate response will be very much appreciated
Thanks a lot!! it is very usefull!!! =D
Hi, i'm using gelanalyzer for the first time but can it accept decimals in the MW calibration mode? and also in the position results?
perfect
would you mind to help me with a bit problem I have? The thing is that I do not understand how can I get the amplicon size exactly in each PCR band. Can you help me on this? Thank you!!
How do you do cal volume? I didn't see that in the example and I'm lost on that.
thanks a lot man :D
Hi,from this software how can we determine teh concentration ng/ul
I was looking for more info on quantitation. I am less interested in migration or size determination... that's what a marker is for and can easily be determined by eye most of the time. The reason I use this type of software is for band intensity comparison as a means to quantitate concentration. This is a common practice using ImageJ. I was hoping to find the equivalent method for this software in this video. Do you have other videos that explain quantitation, including dilution factors to accommodate for the volumes loaded in the marker?
Hi Casey. Its been many years since I posted this tutorial, and I haven't needed to work with it lately, so I may not be the best person to ask about this. What you are doing in terms of reverse-engineering the concentration seems interesting, but outside of the intensity listed on the y-axes I don't know of a means to do this with this particular software suite off the top of my head. That said, I would definitely recommend reaching out to Istvan Lazar, who is the main developer and who has continued adding enhancements to the software suite over the years. You should be able to find the contact information for him as well as others on the team at the link I provided in the description.
I have some question about Gel Analyzer.
So when I switch to MW calibration mode and I write the values for the molecular weights it seems it can't save what I write. I hovered over the dot, wrote base pair and pressed enter but it did't take.
What could be the problem?
Thank you for your help. :)
It has been a while since I made this video (my apologies to everyone whose questions I've missed over the years), but from what I can remember this sounds very similar to what happened to me at 6:36 in the video. Pressing "Enter" with NumLock enabled on my keypad worked from what I can recall. If that's not the case for you, then I would try troubleshooting different file formats (.jpg, .png, .bmp) to see if that makes any difference. As a last resort, maybe uninstalling and reinstalling GelAnalyzer may correct your issue.
can someone explain the curve please
Where from you add molecular weight values???
It's been about 5 years since I made this video, but I do remember that the MW values are typically standard and known ahead of time. I was reading off of a reference paper from the lab.
Not really explained. You speak words like "values" and dunno, - but what values? What? Why? When? Or Why do you manually select bands? What is the point using this kind of software when you are detecting your bands manually? Ah.
This video was made over 7 years ago. I haven't looked recently, but I wouldn't be surprised if the more recent versions of this program (or others like it) have improved a lot and require less manual work. Nowadays, it is even faily trivial to train a basic image recognition model using basic libraries and frameworks from Python.