Using ImageJ to measure polyacrylamide gel images

Download ImageJ from the NIH here: imagej.nih.gov/ij/download.html
3:30 - How to download ImageJ
5:10 - Installation Windows users
8:30 - Installation for Mac users
11:48 - How to open your TIFF file on ImageJ and set rotation & dimensions
19:10 - Recognize your ladder
28:15 - Using the ROI manager and begin drawing and adding lines
40:01 - Saving and accessing your work
43:13 - Extract measurements and save as a .csv file
48:39 - Open file in Excel and generate your standard curve

[@LovelyLife365]

Thanks for your easily described training video.

[@chakkravarthyv8333]

very nice video. Thanks a lot

[@SalouaMABSOR]

so useful, thanks

[@divyanshutiwari9916]

The explanation is wonderful, if u can brief how can I identify a band of particular size on the gel?

[@tarabridgetpierre573]

Thank you so much for this video!! Quick question: Is the LDH going to be present on the standard curve?

[@2women88]

what is the reason for changing from inches to meters, please?

[@hananalenzi436]

Hello. Can you please give me your email because I need your help with image j. Thank you

[@thebubiochemistrylaborator2333]

Hello! You can e-mail us at [e-mail]

[@livinghope8039]

long, complex and boring!