Using PyMOL for protein mutagenesis
HTML-код
- Опубликовано: 9 сен 2024
- For Chapter 11C, you will be using the PyMOL protein modeling program to perform a point mutation in an effort to modify an already existing hydrogen bond interaction.
00:22 Installing PyMOL
01:38 Using the Tutorial
02:32 Your asssigned protein (Prompt 1 & 2)
05:07 Search for ligand (Prompt 3)
13:26 Loading and customizing your protein (Prompt 4)
20:02 Capturing Screenshots and Saving work (Prompt 4)
21:23 Using PDB site to find an amino acid of interest (using 3D-interaction page 10:44) (Prompt 5)
30:32 Measuring Hydorgen bond distances (Prompt 5)
34:45 Performing mutagenesis (Prompt 6)
41:54 Final notes (Conclusions section)
great video thank you, but why would PDB website show asparate reside at site 31, and yet when we import the sequence into PyMOL its at site 32?
many thanks, very useful.
How to save the mutagenesis into PDB file to the computer from Pymol?
file > export molecule > choose pdb as a file format
bagaimana cara menentukan mutasi terbaik dari asam amino? salam dari indonesia pak. semoga sehat selalu