Using PyMOL for protein mutagenesis

For Chapter 11C, you will be using the PyMOL protein modeling program to perform a point mutation in an effort to modify an already existing hydrogen bond interaction.
00:22 Installing PyMOL
01:38 Using the Tutorial
02:32 Your asssigned protein (Prompt 1 & 2)
05:07 Search for ligand (Prompt 3)
13:26 Loading and customizing your protein (Prompt 4)
20:02 Capturing Screenshots and Saving work (Prompt 4)
21:23 Using PDB site to find an amino acid of interest (using 3D-interaction page 10:44) (Prompt 5)
30:32 Measuring Hydorgen bond distances (Prompt 5)
34:45 Performing mutagenesis (Prompt 6)
41:54 Final notes (Conclusions section)

[@stephenchapman8603]

great video thank you, but why would PDB website show asparate reside at site 31, and yet when we import the sequence into PyMOL its at site 32?

[@maryamsediqi3726]

many thanks, very useful.

[@shravyagl7051]

How to save the mutagenesis into PDB file to the computer from Pymol?

[@khalidhelmy9852]

file > export molecule > choose pdb as a file format

[@zaenalwatoni2488]

bagaimana cara menentukan mutasi terbaik dari asam amino? salam dari indonesia pak. semoga sehat selalu