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The BU Biochemistry Laboratory Manual
Добавлен 4 июл 2019
Calculating moles of -SH groups and GAPDH in C-14 iodoacetate reaction
Use this sample calculation tutorial videos to calculate the moles of cysteine residues and GAPDH in your iodoacetate labeling reactions
Просмотров: 622
Видео
Using ImageJ to measure polyacrylamide gel images
Просмотров 25 тыс.3 года назад
Download ImageJ from the NIH here: imagej.nih.gov/ij/download.html 3:30 - How to download ImageJ 5:10 - Installation Windows users 8:30 - Installation for Mac users 11:48 - How to open your TIFF file on ImageJ and set rotation & dimensions 19:10 - Recognize your ladder 28:15 - Using the ROI manager and begin drawing and adding lines 40:01 - Saving and accessing your work 43:13 - Extract measure...
Calculating number of amino acid residues from pH-stat experiment
Просмотров 3803 года назад
Calculating number of amino acid residues from pH-stat experiment
Calculating theoretical and experimental buffer capacity
Просмотров 1,8 тыс.3 года назад
Calculating theoretical and experimental buffer capacity
Correcting histidine buffer titrant values
Просмотров 2903 года назад
How to subtract your water titration values from your histidine buffer titration values to obtain a corrected histidine titration graph.
Graphing histidine buffer and water titration data
Просмотров 2523 года назад
How to transform your histidine buffer and water titration data to generate a single graph with both curves.
Creating a loading scheme for and SDS polyacrylamide gel
Просмотров 3704 года назад
Creating a loading scheme for and SDS polyacrylamide gel
Calculating number of -SH groups (cysteine residues) per GAPDH molecule
Просмотров 5024 года назад
Calculating number of -SH groups (cysteine residues) per GAPDH molecule
Calculating moles of GAPDH in DTNB reaction
Просмотров 8244 года назад
00:58 - Going through your TF data; Standard Curve and Experimental tube readings (Prompt B-4) (and 15 seconds starting at 5:40) 03:22 - Creating a standard curve (Prompt B-5) 05:18 - Converting absorbance to concentration with error in slope of standard curve (Prompt B-6) 22:12 - Converting concentration to moles with error (Prompt B-7) 34:13 - Averaging moles with error of GAPDH (Prompt B-4)
Calculating moles of -SH groups in your DTNB reaction
Просмотров 1,4 тыс.4 года назад
01:19 - Calculating your DTNB apparent extinction coefficient (Prompt B-1) 09:53 - Calculating the concentration of -SH groups (Prompt B-2) 15:24 - Calculating the moles of -SH groups in your DTNB reaction (Prompt B-3)
Making a table for your GAPDH reaction activity rates
Просмотров 3634 года назад
Making a table for your GAPDH reaction activity rates
Creating a table and graph of your DTNB reactions
Просмотров 7064 года назад
00:05 - Making a table (Prompt A-1) 11:38 - Making a graph (Prompt A-2)
Using PyMOL for protein mutagenesis
Просмотров 5 тыс.4 года назад
For Chapter 11C, you will be using the PyMOL protein modeling program to perform a point mutation in an effort to modify an already existing hydrogen bond interaction. 00:22 Installing PyMOL 01:38 Using the Tutorial 02:32 Your asssigned protein (Prompt 1 & 2) 05:07 Search for ligand (Prompt 3) 13:26 Loading and customizing your protein (Prompt 4) 20:02 Capturing Screenshots and Saving work (Pro...
Testing your Acrobat Reader compatibility with PDF exams
Просмотров 1944 года назад
Students in the Biochemistry II course (BI/CH 422/622) at Boston University will be completing the remainder of their exams online using the Adobe Acrobat Reader program with comments enabled for drawing-type questions.
Preparing scintillation samples
Просмотров 1,6 тыс.4 года назад
Watch a video on how to use the scintillation fluid dispenser, and how to prepare your samples for liquid scintillation using the LSC-6500 model made by Beckman-Coulter
great video thank you, but why would PDB website show asparate reside at site 31, and yet when we import the sequence into PyMOL its at site 32?
Thank you so much for this video!! Quick question: Is the LDH going to be present on the standard curve?
Thanks for your easily described training video.
so useful, thanks
long, complex and boring!
many thanks, very useful.
very nice video. Thanks a lot
bagaimana cara menentukan mutasi terbaik dari asam amino? salam dari indonesia pak. semoga sehat selalu
Nice job Sir
The explanation is wonderful, if u can brief how can I identify a band of particular size on the gel?
what is the reason for changing from inches to meters, please?
How to save the mutagenesis into PDB file to the computer from Pymol?
file > export molecule > choose pdb as a file format
Hello. Can you please give me your email because I need your help with image j. Thank you
Hello! You can e-mail us at biochemlabmanual@gmail.com
Your computer isn't the only one freaking out rn
Thanks Jose! Very Helpful!
Glad it was helpful!
SIR HOW TO MAKE NELSON SOMOGYI REAGENT ??AND HOW TO PREPARE SAMPLE SOLUTION ..?? SIR PLS REPLY...
Hello! You can e-mail us at biochemlabmanual@gmail.com
can i get this explanation too, please?@@thebubiochemistrylaborator2333