How to make Western Blot gel

[@tabbico]

Great trick the parafilm under the gel😍

[@yuanxu656]

The way that they did it would make the glass plates dislocate tho

[@mostirreverent]

I never had problems with the biorad system leaking, unless the glass had chips. Owl scientific let you cast on the running device using an agarose plug

[@Yhusi]

Thanks for the nice video. Lately sometime I fail to make perfect well, and I realize it when I load my sample it won’t go through coz there are this sheet of the gel between the well. Might anyone have a suggestion? Many thanks

[@NetworkNetwork-m4j]

Till where do you add the stacking gel? How much are you pouring in?

[@nathaliecedeno5251]

Thank you for sharing!

[@rajdipmisra4525]

Just one question, for a 40Kd protein which is ideal? 19:1 Acrylamide or 39:1 Acrylamide?

[@monday2471]

thank you!!