Invitro seed germination process, surface sterilization techniques & tips to avoid browning of media

Watch this video to know how to germinate seeds in plant tissue culture laboratory with special emphasis on seed dominance, methods to break seed dominance (scarification) and get some tips to avoid browning of media due to phenolic exudates released from the seeds.
Do like, share and subscribe.
Contact me at [e-mail]
Thank you

[@komalkhedkar6029]

Keep uploading more videos like this , sir👍

[@srinivasmohanty8859]

Thanks Bro for sharing the process and techniques of in-vitro seed germination . I want to give 100 likes....

[@sanket3782]

Nice video
👌👌👌

[@kartikpal3196]

Awesome stuff!! Keep it up bro

[@srinivasug6649]

Nice video bro..

[@AD-hs9su]

Thank you so much..

[@anshikagarg1644]

Sir. Please make one video for how seed is coated with microbial strain which protocol is used??

[@anshikagarg1644]

Sir for coated seeds which type of nutritional test are done???? Like protein.... ???

[@rajendarreddyreddy3774]

Sir. I want agrobacterium c carrot 🥕 hairy root.. video.

[@ourtamilnadu9011]

👍👍👍👍👍👍👍

[@srinivasmohanty8859]

Is every seed produces phenolic compounds? I have prepared the germination of seeds of mustard,gram etc but didn't seen the brown colour in media .
BTW in the lab u r alone working!! What r u doing bro?

[@malthehansen7915]

A question sir, with the addition of the activated carbon, what happens to the PGRs needed in media?
Do the Auxins, Gibberellins & Cytokinins not get absorbed?
Also can you please provide the papers for us Novices, thank you sir

[@Dr.Rakesh.B]

Yes, a little amount of hormones do get absorbed. Thats why we keep changing media every week. And we stop adding charcoal after few cycles.
You can contact me at [e-mail] for reference papers

[@jadavsagar1983]

Can tell me washing bottle techniques

[@Dr.Rakesh.B]

Just autoclave for decontamination and wash with slight detergent and rinse with distilled water and dry it in hot air oven at 50 - 60 degrees for a day. Watch my video on how to avoid contamination for more ideas

[@NoLandMandi]

what is that "plant tissue culture" is there any way to make that at home?

[@Dr.Rakesh.B]

Yes so many people do that at home for passion as hobby. In Facebook there's many group you can search and join to that

[@mariaboban3560]

Which hormone used

[@Dr.Rakesh.B]

Giberellic acid

[@dhivyaa_diya]

Sir can u please sujjest me the best composition of Ms media for tomato seed culture invitro ..

[@dhivyaa_diya]

@@Dr.Rakesh.B thank you 😊

[@pallavipanari3617]

Why we autoclave Hgcl2?

[@Dr.Rakesh.B]

The water we use to prepare the solution might have some contaminants. So its advised to autoclave

[@ZK292]

I want to read UR reasearch paper sir.....

[@Dr.Rakesh.B]

You can follow me in research Gate (Rakesh Bhaskar) or Google Scholar (Dr. Rakesh)