PureBlu™ DAPI Nuclear Staining Dye for Fixed Cells - A Fast Approach to Staining Nuclei

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This brief tutorial demonstrates the use of PureBlu DAPI Dye with the ZOE™ Fluorescent Cell Imager for routine nuclear staining in fluorescence microscopy and cell imaging applications.
PureBlu Nuclear Staining Dyes are designed to specifically stain the nuclei of cells in fixed and unfixed samples for fluorescence microscopy and cell imaging applications.
Based on the well-recognized DAPI and Hoechst 33342 chemistries, PureBlu Dyes are offered in a ready-to-reconstitute, high-purity powder format. PureBlu DAPI and PureBlu Hoechst 33342 Dyes are both ready to use after a single dilution - no weighing required.
Features and Benefits of PureBlu Nuclear Staining Dyes
• High purity formulation for quality results in challenging experiments
• Pre-aliquoted powder format eliminates time-consuming weighing steps
• Convenient format allows you to generate working solution with only one dilution step after resuspension
• Compatible with multicolor experiments
PureBlu Nuclear Staining Dye One-Step Dilution
Resuspend one tube of PureBlu Dye Powder in 500 µl of deionized H2O and dilute to
50 ml in PBS/media to prepare the working solution (PureBlu DAPI, 1 µg/ml (0.03 mM);
PureBlu Hoechst 33342, 1.1 µg/ml (0.02 mM)).
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[@rrfarfan]

Very useful explanation. Thank you

[@rashmimohanty2847]

Why DAPI only binds to the minor groove AT rich region, And not GC regions? What brings such a great affinity that it's a specific property of DAPI dye. ?

[@michaelarson9616]

Woow cool learn info from student mit edu.
Thank you

[@anniem.k.9431]

how does a dead cell that underwent apoptosis appear under the microscope? How to differentiate them from the healthy cells?

[@thefenerbahcesk4156]

Why doesn't DAPI stain mitochondria?

[@novabiotis3993]

Dapi stains all DNA and for instance you can see blue little dots on cell culture dishes when they are contamined with mycoplama bacteria. Mitochondrions are also stained but the DNA is small and is not as easy to visualise than the large eucaryotic nuclei.

[@thefenerbahcesk4156]

@@novabiotis3993 I guess that explains why DAPI staining is so bad in yeast cells. The whole cell is blue and I can just barely see a darker blue where the nucleus is.

[@novabiotis3993]

@@thefenerbahcesk4156
Yeast are the smallest eucaryotic cells ! Only 5 micromètres.. so, il your staining is too strong, all thé cytosol will be fluorescent .