PureBlu™ Hoechst 33342 Nuclear Staining Dye for Live Cells - A Fast Approach to Staining Nuclei

For more information, visit www.bio-rad.com...
This brief tutorial demonstrates the use of the PureBlu Hoechst 33342 Dye with the ZOE™ Fluorescent Cell Imager for routine nuclear staining in fluorescence microscopy and cell imaging applications.
PureBlu Nuclear Staining Dyes are designed to specifically stain the nuclei of cells in fixed and unfixed samples for fluorescence microscopy and cell imaging applications.
Based on the well-recognized DAPI and Hoechst 33342 chemistries, PureBlu Dyes are offered in a ready-to-reconstitute, high-purity powder format. PureBlu DAPI and PureBlu Hoechst 33342 Dyes are both ready to use after a single dilution - no weighing required.
Features and Benefits of PureBlu Nuclear Staining Dyes
• High purity formulation for quality results in challenging experiments
• Pre-aliquoted powder format eliminates time-consuming weighing steps
• Convenient format allows you to generate working solution with only one dilution step after resuspension
• Compatible with multicolor experiments
PureBlu Nuclear Staining Dye One-Step Dilution
Resuspend one tube of PureBlu Dye Powder in 500 µl of deionized H2O and dilute to
50 ml in PBS/media to prepare the working solution (PureBlu DAPI, 1 µg/ml (0.03 mM);
PureBlu Hoechst 33342, 1.1 µg/ml (0.02 mM)).
www.bio-rad.com...
We Are Bio-Rad.
Our mission: To provide useful, high-quality products and services that advance scientific discovery and improve healthcare. At Bio-Rad, we are united behind this effort. These two objectives are the driving force behind every decision we make, from developing innovative ideas to building global solutions that help solve our customers' greatest challenges.
Connect with Bio-Rad Online:
Website: www.bio-rad.com/
LinkedIn: / 1613226
Facebook: / biorad
Twitter: / bioradcellbio
Instagram: @BioRadLabs
Snapchat: @BioRadLabs

[@pujapatel2271]

Please upload a video for any IC50 treated cell with hoechst stain. I want to know the exact difference between hoechst stained live and apoptotic cells.

[@kevinrobledo856]

It is posible to stain the DNA of e. coli with this technic?