Surface and Intracellular Cytokine Staining for Flow Cytometry

This protocol video provides a list of the reagents required and the steps needed to perform flow cytometry staining. Within the video, we cover fixation, permeabilization, and both surface and intracellular staining with antibodies. Learn these essential steps so that you stain cells successfully with complex multicolor flow cytometry panels.

[@itssushmita687]

Can we incubate FITC conjugated antibody in the Co2 incubator