Flow Cytometry Protocol for Staining Membrane Associated Proteins

This is a step-by-step video protocol on how to perform flow cytometry using Bio-Techne's conjugated antibodies to stain membrane-associated proteins in suspended cells. From this video, you will be guided through: cell preparation, experimental setup, FC receptor blocking, conjugated antibody addition and incubation, wash steps, and cell resuspension.
View full written version of this protocol: www.rndsystems.com/resources/...
Other flow cytometry staining protocols: www.rndsystems.com/resources/...
Flow cytometry workflow solutions:
www.rndsystems.com/products/f...
Need conjugated antibodies?
R&D Systems: www.rndsystems.com/search?key...
Novus Biologicals: www.novusbio.com/product-type...

[@mamostamuhamad7326]

thanks for this video

[@MrBepis-qx5ye]

What is the purpose of phosphate buffered saline?

[@user-sm5ww2ne9x]

it mimics physiologic pH and ion concentration