You're the man, Doc! Priceless info! Concise delivery! Great presentation, Doc! Thank you for doing this awesome video! Questions: 1. So, a haploid is always going to be a monokaryon? 2. 2 monokaryons meeting each other result in a dikaryotic state which could form a dikaryon or always forms a new dikaryon through their union (n + n = (n + n))? 3. 2 part question (about the following wiki article: "This The development of rhizomorphs begins with a submerged thallus that produces mycelium (hyphae biomass) that when deprived of nutrients and exposed to increasing oxygen, morphogenesis occurs giving rise to pseudo or microsclerotia (survival structures of some fungi), which precede rhizomorph development. Concentrations of oxygen play an important role in the production of rhizomorphs. When there is a high concentration of oxygen in the atmosphere, soil moisture, temperature and pH, rhizomorph production increases. Rhizomorphs contain four differentiated types of tissues: The outer layers are a compact growing point that make up the mucilage, The melanized wall that serves as protection against colonization by another microorganisms (bacteria or fungi), The medulla that serves for conduction of water and dissolved nutrients, The central line used as an air conducting channel.") 3a. would having a little more oxygen access by just a microflow allow for more rhizomorphic manifestations in your plate? 3b. Would presenting a source of vitamin A and C in the agar recipe (perhaps in the moisture or substrate also) help ensure the production of robust melanized walls to increase its probability to survive to fruition more effectively under wider limitation parameters instead of otherwise? 4. can we use a food dye in our agar to get the color we want? 5. How do you know when you have isolated a genome or have a monoculture? is it a question of dilution of the genomes to call a monoculture after the T11? If so, the smaller the size of the mycelium transferred the faster it will be "diluted" so you may achieve this by t5? Could we go microscopically to select our biopsies? if so, what would you look for when selecting your samples? 6. identification of mushrooms can be classified by phenotypes or genotypes, is that right? a lot of people argue for genotypes, including myself until now since, if a single plate of haploids coming from one fruit ends up with thousands of different genotypes, then how could you not revert back to identifying the mushroom by phenotypes for simplification, practicality, and feasibility's sake as it would be an endless list of genotypes for some fruits with the same phenotype? 7. what is the purpose of isolating a genome? what could you do with that? I know you are busy af, you don't have to answer any of it, it's totally cool. PS: Thank you for recommending Chiang Mai, it looks like a comfy city. It appears to offer yummy meals, and is very dog-friendly! My great dane's gonna have some Thai doggy friends! Thanks, boss! Though, I'm still studying the move... It seems like there are some changes on the horizon over there after what I hear in the news... we'll see. That's another subject. Peace, Doc!
Thankyou for clarifying all the naming conventions and explaining them properly. Im sure ive got it wrong many times with only 6 months experience..btw ive added tou on FB.
Thanks Ed...learning a ton thanks to your vids. Was wondering if you can describe (or do a vid on) visually identifying an monokaryon vs. a dikaryon on a plate? Thanks!
What if they didnt isolate the culture before making an LC, and they just added piece from the first plate ? Another question, we propagate the culture many times so we can get a monoculture, which , if visibly described, would look like a single propagation of hyphae ?
It would just be a mixture of cultures, most likely fighting with each other. A monoculture should have uniform, radial growth with no sectoring. Most of the time...
Hey Ed! Not sure how I missed this one until now, but I already learned all that from your numerous other vids :) One thing thought that I think might still be a little confusing for beginners, which I am, is that point you clarified on haploid/diploid nuclei... I know I was confused for a long time, think I figured it out and wanted to run it by you. Your note still kinda gives the feeling the term monokaryon is associated with haploid, and dikaryon with diploid.. apart from the (). So just to be clear, would it be fair to summarise this way? Two haploid monokaryons mate to form a haploid dikaryon (each nucleus still contains half the genetic material the final fruit will be made of). We only see diploid nuclei after karyogamy and before meiosis, in the basidium, during the process of spore creation. And that is the only stage at which cross-over occurs, as in the genetic roulette :) I'm sure this is a crude summarisation for a scientist like you, but you get what I mean.. Anything hurting your eyes in there?
Yes. That is correct. The only part is about calling the new dikaryon a 'haploid'. Haploid implies a cell that on had half of the normal genetic compliment. i.e. 'n', not n+n, 2n, 3n, etc. I have heard other scientists call it a 'polyploid', 'heterokaryon', 'multi-nucleate', etc. I would stick with calling it a dikaryon which is a special term we have in fungi. It eliminates any ambiguity. Sounds like you have been learning very well. This is precisely what I want to do with all these videos. You all will be making the videos soon. :) Thank you for watching!
Question Mr. Ed if you happen to see this....if I was still green as hell and wet behind the ears but interested in dipping my feet into the mycology world a bit more with the purchase of new scope, what would you recommend as a good practice or use for said scope when first starting out. What should I be using the scope for if I'm just a backyard, homegrown, self taught hobbyist looking to expand his understanding of this new hobby. Thanks in advance good sir!
Amscope B120C. It is all you need for checking monokaryons, dikaryons, measuring spores, etc. If you get into identifying wild specimens, it is all you need. I think they are going for about $250 now. It will be the only scope you ever need to buy for your lifetime in Mycology. Hobby or semi-pro.
Ed, learning so so much. Thank you. Going to switch over to you bagging system. How do you sterilize: PC or just steam at ambient pressure ?? Your bags sound like cellophane, So Amazon has these: 12” X 18” 1.1 Mil LDPE Open Ended & Flat Clear Poly Bags, glossy, THIN & LIGHTWEIGHT: Manufactured from virgin LDPE (low-density) material. HEAT SEALABLE ( impulse sealer ), 100% FOOD SAFE If these are not suitable or the same please would you be kind enough to provide an accurate description of the bags you use. Thx again.
@@edwardgrand perfect and short concise. Love it. Thanks again for saving me time, bags, and grain. I asked about pc or steam bc I had purchased some bags that melted ( ish ) and fused into one large block. What a pain that was :(
![[Limbus Company - PV] Chapter.7 - Oblivion](http://i.ytimg.com/vi/VUpkXPkvoxU/mqdefault.jpg)
Yeees just put on the camera and start talking - that's it! 😍 Love the loads of videos you're bashing out 🔥🔥🔥
You're the man, Doc! Priceless info! Concise delivery! Great presentation, Doc! Thank you for doing this awesome video!
Questions:
1. So, a haploid is always going to be a monokaryon?
2. 2 monokaryons meeting each other result in a dikaryotic state which could form a dikaryon or always forms a new dikaryon through their union (n + n = (n + n))?
3. 2 part question (about the following wiki article: "This The development of rhizomorphs begins with a submerged thallus that produces mycelium (hyphae biomass) that when deprived of nutrients and exposed to increasing oxygen, morphogenesis occurs giving rise to pseudo or microsclerotia (survival structures of some fungi), which precede rhizomorph development. Concentrations of oxygen play an important role in the production of rhizomorphs. When there is a high concentration of oxygen in the atmosphere, soil moisture, temperature and pH, rhizomorph production increases.
Rhizomorphs contain four differentiated types of tissues:
The outer layers are a compact growing point that make up the mucilage,
The melanized wall that serves as protection against colonization by another microorganisms (bacteria or fungi),
The medulla that serves for conduction of water and dissolved nutrients,
The central line used as an air conducting channel.")
3a. would having a little more oxygen access by just a microflow allow for more rhizomorphic manifestations in your plate?
3b. Would presenting a source of vitamin A and C in the agar recipe (perhaps in the moisture or substrate also) help ensure the production of robust melanized walls to increase its probability to survive to fruition more effectively under wider limitation parameters instead of otherwise?
4. can we use a food dye in our agar to get the color we want?
5. How do you know when you have isolated a genome or have a monoculture? is it a question of dilution of the genomes to call a monoculture after the T11? If so, the smaller the size of the mycelium transferred the faster it will be "diluted" so you may achieve this by t5? Could we go microscopically to select our biopsies? if so, what would you look for when selecting your samples?
6. identification of mushrooms can be classified by phenotypes or genotypes, is that right? a lot of people argue for genotypes, including myself until now since, if a single plate of haploids coming from one fruit ends up with thousands of different genotypes, then how could you not revert back to identifying the mushroom by phenotypes for simplification, practicality, and feasibility's sake as it would be an endless list of genotypes for some fruits with the same phenotype?
7. what is the purpose of isolating a genome? what could you do with that?
I know you are busy af, you don't have to answer any of it, it's totally cool.
PS: Thank you for recommending Chiang Mai, it looks like a comfy city. It appears to offer yummy meals, and is very dog-friendly! My great dane's gonna have some Thai doggy friends! Thanks, boss! Though, I'm still studying the move... It seems like there are some changes on the horizon over there after what I hear in the news... we'll see. That's another subject. Peace, Doc!
bro i love your personality, its like adhd on crack but i understood everything!
Thank you!
You answered it. Danke
You’ve been killing it lately Ed! Absolutely love all you do!
Great great video Ed! I’m learning 😂
nice one again. can you maybe do a video on reviving dried fruits? keep it up brother. really appreciate your videos
Awesome info. Thank you 🌞🍄
Great info Ed Thanks for the explanation
The more you know!! Love it Ed!!
Very interesting. Thank you
Thanks for the info! I'm getting smarter by
Thankyou for clarifying all the naming conventions and explaining them properly. Im sure ive got it wrong many times with only 6 months experience..btw ive added tou on FB.
that clears things up a lot, thanks Ed!!!
Thanks for the video. I'm just getting into agar and this helps.
Thanks Ed your knowledge is always spot on top notch. Love your work always 🙏🍄👍
Thanks Ed
I love the topic, definitely needed
Thanks Ed...learning a ton thanks to your vids. Was wondering if you can describe (or do a vid on) visually identifying an monokaryon vs. a dikaryon on a plate? Thanks!
I have. It is very hard to do the videography with my setup.
Monokaryons don't have clamps at any septa. Dikaryons have clamps at some or many septa.
Thanks bro its hard to find all this information in one place. 🍄❤️
What is the benefit of a monoculture vs. any other.
What if they didnt isolate the culture before making an LC, and they just added piece from the first plate ?
Another question, we propagate the culture many times so we can get a monoculture, which , if visibly described, would look like a single propagation of hyphae ?
It would just be a mixture of cultures, most likely fighting with each other.
A monoculture should have uniform, radial growth with no sectoring. Most of the time...
🎉😊
Hey Ed! Not sure how I missed this one until now, but I already learned all that from your numerous other vids :)
One thing thought that I think might still be a little confusing for beginners, which I am, is that point you clarified on haploid/diploid nuclei... I know I was confused for a long time, think I figured it out and wanted to run it by you.
Your note still kinda gives the feeling the term monokaryon is associated with haploid, and dikaryon with diploid.. apart from the (). So just to be clear, would it be fair to summarise this way?
Two haploid monokaryons mate to form a haploid dikaryon (each nucleus still contains half the genetic material the final fruit will be made of). We only see diploid nuclei after karyogamy and before meiosis, in the basidium, during the process of spore creation. And that is the only stage at which cross-over occurs, as in the genetic roulette :)
I'm sure this is a crude summarisation for a scientist like you, but you get what I mean.. Anything hurting your eyes in there?
Yes. That is correct. The only part is about calling the new dikaryon a 'haploid'. Haploid implies a cell that on had half of the normal genetic compliment. i.e. 'n', not n+n, 2n, 3n, etc. I have heard other scientists call it a 'polyploid', 'heterokaryon', 'multi-nucleate', etc. I would stick with calling it a dikaryon which is a special term we have in fungi. It eliminates any ambiguity. Sounds like you have been learning very well. This is precisely what I want to do with all these videos. You all will be making the videos soon. :)
Thank you for watching!
@@edwardgrand Thank you for teaching us well!
Question Mr. Ed if you happen to see this....if I was still green as hell and wet behind the ears but interested in dipping my feet into the mycology world a bit more with the purchase of new scope, what would you recommend as a good practice or use for said scope when first starting out. What should I be using the scope for if I'm just a backyard, homegrown, self taught hobbyist looking to expand his understanding of this new hobby. Thanks in advance good sir!
Amscope B120C. It is all you need for checking monokaryons, dikaryons, measuring spores, etc. If you get into identifying wild specimens, it is all you need. I think they are going for about $250 now. It will be the only scope you ever need to buy for your lifetime in Mycology. Hobby or semi-pro.
Pinecones represent the pineal they ❤ so it would be like fucked magnets? I've had really shiityness with agar compared to lc. Is the mono/di. ness?
LC and agar both have their purposes. Some better than others.
Ed, learning so so much. Thank you. Going to switch over to you bagging system. How do you sterilize: PC or just steam at ambient pressure ?? Your bags sound like cellophane, So Amazon has these: 12” X 18” 1.1 Mil LDPE Open Ended & Flat Clear Poly Bags, glossy, THIN & LIGHTWEIGHT: Manufactured from virgin LDPE (low-density) material. HEAT SEALABLE ( impulse sealer ), 100% FOOD SAFE
If these are not suitable or the same please would you be kind enough to provide an accurate description of the bags you use. Thx again.
The bags have to be PP5 to hold up in the PC. Grain must be sterilized in PC or autoclave. Thanks for watching.
@@edwardgrand perfect and short concise. Love it. Thanks again for saving me time, bags, and grain. I asked about pc or steam bc I had purchased some bags that melted ( ish ) and fused into one large block. What a pain that was :(
Still very lost listening to this. Only because i don’t know enough. Piece by piece.
There's a lot to learn. Hope it helped. I've been doing this a while and learn something new every day. Always new stuff out there.
Youre aewsone ed
Sheding light on that terminology, makes me sound like I have a degree in mycology 😅