Probe-based qPCR

Probe-based qPCR allows for specific hybridization. The targeted nature of probe-based qPCR leads to low background, or nonspecific fluorescence, and eliminates the presence of false positives. You can also label probes with different, distinguishable reporter dyes to allow for amplification of two distinct sequences in one reaction tube.
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[@shahirkhan6344]

Why Probes/Primers attach first? Why Dna polymerase starts their job after annealing?
Does that mean dna polymerase can't start attaching dntp's without a primer (sequence)?

[@nora930]

DNA polymerase can't start adding nucleotides if there isn't a primer first, otheriwe the polymerase doesn't know where it should start. So yes, primer anneals first on a strand.

[@jolie3246]

the primer is a specific sequence recognized by polymerase as a starting point

[@ppreshants]

@@jolie3246 That is not quite correct.There is no specific recognition of the primer sequence by the polymerase. All the polymerase needs is a double stranded region - specifically the 3' OH group where it could start adding the next dNTP (using a nucleophilic attack / SN2 reaction)

[@zhenzhang8685]

@@ppreshants Totally agreed

[@shahirkhan6344]

Please I need Answer