Discontinuous Diafiltration with Vivaspin®️ Centrifugal Ultrafilters

Buffer exchange is an important technique in most macromolecule purification workflows. It can be used to remove unwanted substances such as salts, adjust sample conditions for optimal capture in a chromatography step, or introduce specific components for various assays or sample storage.
Classical methods for buffer exchange are time-consuming and generate high volumes of waste. In contrast, centrifugal ultrafilters offer easy handling and 90% time savings, while keeping buffer consumption to a minimum.
When the optimal MWCO is selected, Vivaspin®️, Vivaspin®️ Turbo, and Vivacon®️ will retain your target macromolecule inside a filter unit, while buffer low molecular weight solutes pass through a semipermeable membrane. This technique is commonly used for concentrating macromolecules and can be applied to buffer exchange as well, in a process called diafiltration.
This tutorial demonstrates the simplicity of discontinuous diafiltration - an iterative process, where the original buffer is removed through multiple rounds of sample dilution and concentration.
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