Continuous Diafiltration with Vivaspin®️ 20 Diafiltration Cups

Buffer exchange is an important technique in most macromolecule purification workflows. It can be used to remove unwanted substances such as salts, adjust sample conditions for optimal capture in a chromatography step, or introduce specific components for various assays or sample storage.
Classical methods for buffer exchange are time-consuming and generate high volumes of waste. In contrast, centrifugal ultrafilters offer easy handling and 90% time savings, while keeping buffer consumption to a minimum.
When the optimal MWCO is selected, Vivaspin®️ 20 will retain your target macromolecule inside a filter unit, while buffering low molecular weight solutes pass through the semipermeable membranes. This technique is commonly used for concentrating macromolecules and can be applied to buffer exchange as well, in a process called diafiltration.
This tutorial demonstrates how Vivaspin®️ 20 diafiltration cups can make continuous diafiltration a more accessible technique in research laboratories. With no additional equipment requirements and an easy-to-use consumable, buffer exchange can be performed in a rapid, single-step, without the need for multiple spins. Meanwhile, the sample is maintained at a constant volume, ready to use in your next process or to concentrate further by centrifuging the assembly just a little longer.
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