I love it when people map out science in a visual manner, and this diagram is really neat and well-drawn. Makes it way easier to understand and is also nice to look at!
a thing to point out. Naive T Helper cells cannot be activated by a macrophage, they can only be activated by mainly dendritic cells or potentially, B cells
Also, Interferon-gamma increases the expression of HLA-DM. HLA-DO is a negative regulator of HLA-DM by binding to it and inhibiting the release of CLIP
Thank you for doing this, by far the best videos out there on this subject are yours. It is to late to help with my current class, but it would be nice if you could do similar vids while delving a little deeper, cause it seems like every professor out there picks and chooses what parts to teach and what to leave out from there lectures. Example: going into B7.1(2) binding to CD28, and even talking about the cytokines needed for T cell maturation.
Day after tommorrow is my immunology viva, well i had some concept, some doubts which you cleared!!!!!! Thank you soo much! YOU ARE A LIFE SAVER!!!!!!! Stay blessed!!!!!!
Same as my comment for MHCI video - I believe MHCII binding site is not so specific for particular peptide, rather a range of peptides. Only the T cells have the very specific selectivity for antigen due to the genetic rearrangements of the receptor.
I believe you're right. The anchoring part of the peptide actually contains just 1 or 2 anchoring residues. So obviously, a lot of peptides Will fit but most Will not be recognized by t cells.
Thank you so much for pointing this out, I was just starting to wonder this, MHC gene code is just translated without random mixing (all that V, D, C business), correct? So it has a versatile nonspecific binding site?
Great video but I noticed that you said that CD4 binds to the MHC II alpha portion. I know for a fact that this is incorrect and that they bind to the B2 portion specifically.
The D1 portion of CD4 actually binds to the hydrophobic crevice/interface formed between the alpha 2 and beta 2 chains (well away from the TCR binding site)
What will happen if phagolysosome will fuse with a wrong mhc2 molecule endosome .. I mean if peptides of bacteria are not specific for that mhc2 molecule ???? Please reply
At 4:34 he mentions only a specific peptide will bind MHC-II, can someone clarify if he means a given MHC-II molecule will only bind a single peptide -OR- only certain peptides are capable of being bound (if so what dictates this specificity) -OR- if a given APC will only present one epitope of an antigen
purpose of his videos are to give people big pictures of what is going on inside different cells. Those minute details, I am sure you can reinforce in your head when you actually read the book.
But is DC that activate naive CD4 T cells, right? not macrophage. so maybe don't use macrophage as an example of APC, because it can be quite confusing...
really nice teaching. but i think the CD4 binds to the B2 part of MHC or both of B2 and Alfa 2. and what about the role of calnexin and catepsin s here?
Lu beneran orang batak lu? Gw orang batak, bangga banget gw hahha jadi pengen liat muka lu, logat lu, asli internasionale ahhaha anyway, makasih videonya keren banget
Correct me if I'm wrong but, the part where the HLA removes the clip, then the peptides fuse with the endosome, The way that they taught me was, that in order for the HLA to remove the clip, it has to first fuse with the peptide-carrying vesicle. The video is amazing btw (:
I love it when people map out science in a visual manner, and this diagram is really neat and well-drawn. Makes it way easier to understand and is also nice to look at!
a thing to point out. Naive T Helper cells cannot be activated by a macrophage, they can only be activated by mainly dendritic cells or potentially, B cells
2019 and we are still here watching and LEARNING from your videos! Thank you so much for sharing your wealth of knowledge and it breaking down.
Also, Interferon-gamma increases the expression of HLA-DM. HLA-DO is a negative regulator of HLA-DM by binding to it and inhibiting the release of CLIP
Thank you for doing this, by far the best videos out there on this subject are yours.
It is to late to help with my current class, but it would be nice if you could do similar vids while delving a little deeper, cause it seems like every professor out there picks and chooses what parts to teach and what to leave out from there lectures. Example: going into B7.1(2) binding to CD28, and even talking about the cytokines needed for T cell maturation.
Thanks mate, I was searching for a material which could help me understand this problem and the only one was your video, keep doing. Great Job!
this is extremely well explained and mapped out
keep up the good work Armando!! you saved my life ^_^
Day after tommorrow is my immunology viva, well i had some concept, some doubts which you cleared!!!!!! Thank you soo much! YOU ARE A LIFE SAVER!!!!!!! Stay blessed!!!!!!
amazing! the level of detail is just right :)
wow, you've saved my life tonight, ¿thank you so much. Please keep doing this beautiful, excellent work
i know I'm quite randomly asking but does anybody know of a good website to watch newly released series online ?
@Danny Joe i watch on flixzone. You can find it by googling =)
you are honestly suchhhh aaa legend, all your videos have made immuno so easy for me
Really great video, thanks! I snore halfway through when reading these in books, so this was really helpful.
this is brilliant.. very well explained in a way that any student can easily understand.
Your videos help so much! Thank you!
was highkey stressed learning this topic but you made it sm easier on me, thank u
Amazing video... great job!
Thank you so much for your videos.
This was extremely helpful .Usage of drawing to explain the concept is the best way.
thankyou sir.
your art is wonderful!!
Sir, I think CD4 molecule binds with beta2 fragmant of HMC-ll molecule, according to our book.
you are makeing medisn subject more easyer ,, thannk you very much bro
Always great stuff thank you :)
Very detailed. Thank you.
This is so helpful. Thanks for this really easy to understand video.
Excellent video!
Awesome video.
Same as my comment for MHCI video - I believe MHCII binding site is not so specific for particular peptide, rather a range of peptides. Only the T cells have the very specific selectivity for antigen due to the genetic rearrangements of the receptor.
I believe you're right. The anchoring part of the peptide actually contains just 1 or 2 anchoring residues. So obviously, a lot of peptides Will fit but most Will not be recognized by t cells.
I think so too.
Thank you so much for pointing this out, I was just starting to wonder this, MHC gene code is just translated without random mixing (all that V, D, C business), correct? So it has a versatile nonspecific binding site?
Thank you Armando!
Really awesome video 👌👌👌
Thank you. God Bless you.
Thank you! This was very helpful!
Awsm video n great explanation!!!
very good .Yes you r right CD 4 binds only beta 2 chain at a constant domain of mhc class ll
Thank you so much this is very helpful 😍
What happens when the peptide isn't specific for the MHC 2 molecule in the endosome?
You sir, are amazing
so helpful...thanke you you made it so easy for me to understnd
Great video but I noticed that you said that CD4 binds to the MHC II alpha portion. I know for a fact that this is incorrect and that they bind to the B2 portion specifically.
+Andy Pham you're right it binds to B2 portion not alpha
The D1 portion of CD4 actually binds to the hydrophobic crevice/interface formed between the alpha 2 and beta 2 chains (well away from the TCR binding site)
This is exactly what I thought and I've been driving myself mad trying to look its up/confirm elsewhere. Thankyou!
great video. thanks a ton again
Thanks, cool video really helps! However, I think that CD4 actually binds to alpha 2 and beta 2 portion. what do you think?
Is there somewhere I can download your illustrations?
thank you very much
y re a lifesaver thank u!
Thank you!
Thanks Armando
thank you so much
thank you very much....awesome... very nicely explained
you are amazing
could you please describe what is the process after this ? like what's exactly the function of binding MHC-2 with the activated T Helper cell?
Thanks, really helpful
thank yoou for this nice demonstration , i'm having a hard time with this class
Wooooow You are genius thanks so much 😍😍😍😍
Sir ap great ho explain krne m it was very helpful to me
Thank you to much
Great job
Great video. Could you please upload the diagrams at some platform?
What about co-stimulation which is essential for the activation of naive CD4 T-cells
I thank you, man
thank you sir
So the Ii or CD74 protein is the invariant chain that becomes CLIP?
What will happen if phagolysosome will fuse with a wrong mhc2 molecule endosome .. I mean if peptides of bacteria are not specific for that mhc2 molecule ???? Please reply
thank you so much ^-^
Alhamdulillah... Very nice
Perfect thank you
와... Thanks sooooo much😭😭👏🏻👏🏻👏🏻👏🏻
amazing explain
At 4:34 he mentions only a specific peptide will bind MHC-II, can someone clarify if he means a given MHC-II molecule will only bind a single peptide -OR- only certain peptides are capable of being bound (if so what dictates this specificity) -OR- if a given APC will only present one epitope of an antigen
Please do a video on B cell development!!
I not sure about the Ii protein - i'm sure it was the 'Li' protein instead?
Is the Ii protein also known as the invariant chain??
I thought the CD4 binds to the beta domain of the MHC class II molecule?
yeah, i think so as well. I looked it up and it says it binds to the beta-2 domain of the MHC class II molecule.
purpose of his videos are to give people big pictures of what is going on inside different cells.
Those minute details, I am sure you can reinforce in your head when you actually read the book.
Why do you care? Who honestly cares where it binds? Sheesh. Open your mental space
can you please do video on transplant reactions - gvhd
Why is the groove closed in MHC class2🤔
Armando you are awesome! Are your complete drawings available online?
I think they are on facebook
Thank you neil7090
No problem :)
Hello what is the difference between HLA ( human leukocyte antigen ) and MHC ( major histocompaticbility complex ) ?
isn't is oposite MHC is protein HLA is gene
@@Sophie-ts2wq
Thankyouu!
Do dendritic cells use the MHC I processing pathway or MHC II? My textbook says I but my professor says II ._.
All nucleated cells use MHC I
Antigen Presenting Cells, such as Dendritic Cells, also have MHC II
Awesome
But is DC that activate naive CD4 T cells, right? not macrophage.
so maybe don't use macrophage as an example of APC, because it can be quite confusing...
shokran :) شكرا
really nice teaching. but i think the CD4 binds to the B2 part of MHC or both of B2 and Alfa 2. and what about the role of calnexin and catepsin s here?
Shiva Jafari I think that's only for MHC I? MHC II seems to have a different chaperone this time.
CD4 binds to the beta-2 of the MHC II molecule
Calnexin acts as chaperon
Thank uuuu
thanx allot
impressive!!!!!!!!!!!!thnx
wow thankyou:)
Gracias :-)
Good
keep up
10. The role of the HLA system in immune reactions
nice!
I fuck with this MHC II Processing animation. 😎
is this the exogenous process ?
Lu beneran orang batak lu?
Gw orang batak, bangga banget gw hahha jadi pengen liat muka lu, logat lu, asli internasionale ahhaha
anyway, makasih videonya keren banget
Alpha chain is of 29kD and beta is of 34kD. Kindly correct
Made my life easier :p
CD4 binds with beta not alpha
Correct me if I'm wrong but, the part where the HLA removes the clip, then the peptides fuse with the endosome, The way that they taught me was, that in order for the HLA to remove the clip, it has to first fuse with the peptide-carrying vesicle. The video is amazing btw (:
Correct 'Golgi' pronunciation